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Mouse Anti-Human CD14-FITC (UCHM-1)
Cat. No.:
9560-02
FITC Anti-Human CD14 antibody for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
$196.00
| Clone | UCHM-1 |
|---|---|
| Isotype | Mouse (BALB/c) IgG2aκ |
| Isotype Control | Mouse IgG2a-FITC (HOPC-1) |
| Specificity | Human/Cynomolgus CD14 |
| Alternative Names | LPS-R, LPS receptor, gp55 |
| Description | CD14 is a 55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells, and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes. |
| Immunogen | Thymocytes and peripheral blood lymphocytes from a remission AML patient |
| Conjugate | FITC (Fluorescein) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Applications | Flow Cytometry – Quality tested 6-11 Immunohistochemistry-Frozen Sections – Reported in literature 1,2 Immunocytochemistry – Reported in literature 1 Immunoprecipitation – Reported in literature 1 ELISA – Reported in literature 3 Blocking – Reported in literature 4 Depletion – Reported in literature 5 |
| RRID Number | AB_2796927 |
| Gene ID | 929 (Human) 102129342 (Cynomolgus) |
| Gene ID Symbol | CD14 (Human) CD14 (Cynomolgus) |
| UniProt ID | P08571 (Human |
| UniProt Name | CD14_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Hogg N, MacDonald S, Slusarenko M, Beverley PC. Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunology. 1984;53:753-67. (Immunogen, IHC-FS, ICC, IP)
- 2. Mahida YR, Patel S, Jewell DP. Mononuclear phagocyte system of human Peyer's patches: an immunohistochemical study using monoclonal antibodies. Clin Exp Immunol. 1989;75:82-6. (IHC-FS)
- 3. Mendis D, Ginon I, Louis H, McGregor JL, Poston RN. Cell biotinylation provides a sensitive and effective detection technique for cellular adhesion assays: comparison with existing methods. J Immunol Methods. 2001;253:57-68. (ELISA)
- 4. Chu AJ, Moore J, Sime R, Yu WH, Li Z. Antagonism by ethanol of endotoxin-induced tissue factor activation in relation to the depressed endotoxin binding to monocyte-like U937 cells. Cell Biochem Funct. 1997;15:271-81. (Block)
- 5. Lloyd-Jones KL, Kelly MM, Kubes P. Varying importance of soluble and membrane CD14 in endothelial detection of lipopolysaccharide. J Immunol. 2008;181:1446-53. (Depletion)
- 6. Lester EA, Babensee JE. Proinflammatory phenotype of endothelial cells after coculture with biomaterial-treated blood cells. J Biomed Mater Res A. 2003;64:397-410. (FC)
- 7. Yoshida M, Babensee JE. Poly(lactic-co-glycolic acid) enhances maturation of human monocyte-derived dendritic cells. J Biomed Mater Res A. 2004;71:45-54. (FC)
- 8. Babensee JE, Paranjpe A. Differential levels of dendritic cell maturation on different biomaterials used in combination products. J Biomed Mater Res A. 2005;74:503-10. (FC)
- 9. Shankar SP, Babensee JE. Comparative characterization of cultures of primary human macrophages or dendritic cells relevant to biomaterial studies. J Biomed Mater Res A. 2010;92:791-800. (FC)
- 10. Shankar SP, Petrie TA, García AJ, Babensee JE. Dendritic cell responses to self-assembled monolayers of defined chemistries. J Biomed Mater Res A. 2010;92:1487-99. (FC)
- 11. Yoshino N, Ami Y, Terao K, Tashiro F, Honda M. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000;49:97-110. (FC, Cynomolgus Reactivity)
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