My Cart
$0.00
0

Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

  • Home
  • Mouse Anti-Chicken MHC Class II-AF488 (2G11)

Mouse Anti-Chicken MHC Class II-AF488 (2G11)

Cat. No.:
8350-30
Alexa Fluor® 488 Anti-Chicken MHC Class II antibody for use in flow cytometry and immunohistochemistry assays.
$270.00
Size Price (USD) Quantity
0.1 mg $270.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone 2G11
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-AF488 (15H6)
Specificity Chicken/Pigeon/Caiman MHC class II β-chain
Alternative Names B-L
Description The chicken major histocompatibility complex (MHC), or B complex, consists of several clusters of highly polymorphic genes. Like their mammalian counterparts, the avian MHC exerts genetic influence over a variety of important biological functions such as immune response, disease resistance, growth and development, aging, and reproduction. Chicken MHC Class II genes, also known as the B-L subregion, of the chicken MHC encode cell surface glycoproteins that are homologous to mammalian Class II antigens. B-L antigens are structurally similar to mammalian Class II molecules in that they are noncovalently bound dimers of one heavy chain and one light chain. MHC Class II is primarily expressed on B cells and antigen presenting cells (APCs).
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 1,4,6-19
Immunohistochemistry-Frozen Sections – Reported in literature 1,4,20
Electron Microscopy – Reported in literature 5
Immunoprecipitation – Reported in literature 1,2
Purification – Reported in literature 3

RRID Number AB_2796516
Gene ID 693256 (Chicken)
101747454 (Chicken)
Gene ID Symbol BLB1 (Chicken)
BLB2 (Chicken)
Gene ID Aliases B-LB; B-LB21; GSP-BLB1; B-L; B-LB2; B-LBII; GSP-BLB2; S19

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

Citations

  1. 1. Salomonsen J, Dunon D, Skjødt K, Thorpe D, Vainio O, Kaufman J. Chicken major histocompatibility complex-encoded B-G antigens are found on many cell types that are important for the immune system. Proc Natl Acad Sci USA. 1991;88:1359-63. (IP, IHC-FS, FC)
  2. 2. Kaufman J, Skjoedt K, Salomonsen J, Simonsen M, Du Pasquier L, Parisot R, et al. MHC-like molecules in some nonmammalian vertebrates can be detected by some cross-reactive xenoantisera. J Immunol. 1990;144:2258-72. (IP, Caiman Reactivity)
  3. 3. Salomonsen J, Marston D, Avila D, Bumstead N, Johansson B, Juul-Madsen H, et al. The properties of the single chicken MHC classical class II α chain (B-LA) gene indicate an ancient origin for the DR/E-like isotype of class II molecules. Immunogenetics. 2003;55:605-14. (Purification)
  4. 4. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (IHC-FS, FC)
  5. 5. del Cacho E, Gallego M, Lee SH, Lillehoj HS, Quilez J, Lillehoj EP, et al. Induction of protective immunity against Eimeria tenella infection using antigen-loaded dendritic cells (DC) and DC-derived exosomes. Vaccine. 2011;29:3818-25. (EM)
  6. 6. Ekino S, Riwar B, Kroese FG, Schwander EH, Koch G, Nieuwenhuis P. Role of environmental antigen in the development of IgG+ cells in the bursa of fabricius. J Immunol. 1995;155:4551-8. (FC)
  7. 7. Katevuo K, Vainio O. Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery. Immunology. 1996;89:419-23. (FC)
  8. 8. Madruga J, Koritschoner NP, Diebold SS, Kurz SM, Zenke M. Polarised expression pattern of focal contact proteins in highly motile antigen presenting dendritic cells. J Cell Sci. 1999;112:1685-96. (FC)
  9. 9. Koritschoner NP, Madruga J, Knespel S, Blendinger G, Anzinger B, Otto A, et al. The nuclear orphan receptor TR4 promotes proliferation of myeloid progenitor cells. Cell Growth Differ. 2001;12:563-72. (FC)
  10. 10. Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)
  11. 11. Juul-Madsen HR, Dalgaard TS, Røntved CM, Jensen KH, Bumstead N. Immune response to a killed infectious bursal disease virus vaccine in inbred chicken lines with different major histocompatibility complex haplotypes. Poult Sci. 2006;85:986-98. (FC)
  12. 12. Del Cacho E, Gallego M, Lillehoj HS, López-Bernard F, Sánchez-Acedo C. Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009;129:66-75. (FC)
  13. 13. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)
  14. 14. Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek's disease virus. Viral Immunol. 2009;22:321-7. (FC)
  15. 15. Sunkara LT, Achanta M, Schreiber NB, Bommineni YR, Dai G, Jiang W, et al. Butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide gene expression. PLoS One. 2011;6(11):e27225. (FC)
  16. 16. Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. (FC)
  17. 17. Chrząstek K, Piasecki T, Wieliczko A. Impact of CpG oligodeoxynucleotide stimulation on percentage of T and B cells in chicken. Pol J Vet Sci. 2013;16:551-4. (FC)
  18. 18. Fernandes Filho T, Fávaro C Jr, Ingberman M, Beirão BC, Inoue A, Gomes L, et al. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis. 2013;57:671-6. (FC)
  19. 19. Vu Manh T, Marty H, Sibille P, Le Vern Y, Kaspers B, Dalod M, et al. Existence of conventional dendritic cells in Gallus gallus revealed by comparative gene expression profiling. J Immunol. 2014;192:4510-7. (FC)
  20. 20. Olias P, Meyer A, Klopfleisch R, Lierz M, Kaspers B, Gruber AD. Modulation of the host Th1 immune response in pigeon protozoal encephalitis caused by Sarcocystis calchasi. Vet Res. 2013;44:10. (IHC-FS, Pigeon Reactivity)
See All References

Related Products

Related Products

Related Products
Check items to add to the cart or
  1. FLISA plate was coated with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-UNLB (SB Cat. No. 1070-01), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-UNLB (SB Cat. No. 1080-01), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-UNLB (SB Cat. No. 1090-01), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-UNLB (SB Cat. No. 1100-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG<sub>1</sub>-AF488 (SB Cat. No. 0102-30) was captured and fluorescence intensity quantified.
    Mouse IgG1-AF488 (15H6)
    0102-30
    $196.00
  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
    0100-01
    $45.00
  3. Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken MHC Class I-PE (SB Cat. No. 8345-09).
    Mouse Anti-Chicken MHC Class I-PE (F21-2)
    8345-09
    $237.00
  4. Mouse Anti-Chicken MCAM-BIOT (C264)
    Mouse Anti-Chicken MCAM-BIOT (C264)
    8385-08
    $336.00
  5. Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD44-SPRD (SB Cat. No. 8400-13).
    Mouse Anti-Chicken CD44-SPRD (AV6)
    8400-13
    $286.00
  6. Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken Bu-1-AF647 (SB Cat. No. 8395-31) and Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02).
    Mouse Anti-Chicken Bu-1-AF647 (AV20)
    8395-31
    $289.00
  7. Adherent cells of chicken PBMC were stained with Mouse Anti-Chicken Monocyte/Macrophage-PE (SB Cat. No. 8420-09) and anti-Ch-7TM followed by a secondary antibody and DAPI.<br/>Image from Chen YS, Wu HC, Shien JH, Chiu HH, Lee LH. Cloning and characterization of a 7 transmembrane receptor from the adherent cells of chicken peripheral blood mononuclear cells. PLoS One. 2014;9(1):e86880. Figure 4(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Mouse Anti-Chicken Monocyte/Macrophage-PE (KUL01)
    8420-09
    $237.00