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  • Neutralite Avidin-AP

Neutralite Avidin-AP

Cat. No.:
7200-04
Neutralite Avidin-AP for use in ELISA, immunohistochemistry / immunocytochemistry, ELISpot, and western blot assays.
$190.00
Size Price (USD) Quantity
1.0 mL $190.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Specificity Biotin
Source Neutralized (pI = 6.3 ± 0.3), deglycosylated avidin purified from egg white
Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested 1-9
FLISA – Quality tested
Flow Cytometry – Quality tested 2,10,12-14
ELISpot – Reported in literature 3,10,11
Immunohistochemistry-Frozen Sections – Reported in literature 10,15-18
Immunohistochemistry-Paraffin Sections – Reported in literature 19
Immunocytochemistry – Reported in literature 20,21
Western Blot – Reported in literature 4,22-24
Microarray – Reported in literature 25

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Brown MP, Topham DJ, Sangster MY, Zhao J, Flynn KJ, Surman SL, et al. Thymic lymphoproliferative disease after successful correction of CD40 ligand deficiency by gene transfer in mice. Nat Med. 1998;4:1253-60. (ELISA)
  2. 2. Reinagel ML, Taylor RP. Transfer of immune complexes from erythrocyte CR1 to mouse macrophages. J Immunol. 2000;164:1977-85. (ELISA, FC)
  3. 3. Hodge LM, Marinaro M, Jones HP, McGhee JR, Kiyono H, Simecka JW. Immunoglobulin A (IgA) responses and IgE-associated inflammation along the respiratory tract after mucosal but not systemic immunization. Infect Immun. 2001;69:2328-38. (ELISA, ELISPOT)
  4. 4. Wang S, Hirschberg R. BMP7 antagonizes TGF-β-dependent fibrogenesis in mesangial cells. Am J Physiol Renal Physiol. 2003;284:1006-13. (ELISA, WB)
  5. 5. Darniot M, Pitoiset C, Petrella T, Aho S, Pothier P, Manoha C. Age-associated aggravation of clinical disease after primary metapneumovirus infection of BALB/c mice. J Virol. 2009;83:3323-32. (ELISA)
  6. 6. Prager G, Stefanski V, Hudson R, Rödel HG. Family matters: maternal and litter-size effects on immune parameters in young laboratory rats. Brain Behav Immun. 2010;24:1371-8. (ELISA)
  7. 7. Weinstein JS, Delano MJ, Xu Y, Kelly-Scumpia KM, Nacionales DC, Li Y, et al. Maintenance of anti-Sm/RNP autoantibody production by plasma cells residing in ectopic lymphoid tissue and bone marrow memory B cells. J Immunol. 2013;190:3916-27. (ELISA)
  8. 8. Buckner LR, Amedee AM, Albritton HL, Kozlowski PA, Lacour N, McGowin CL, et al. Chlamydia trachomatis infection of endocervical epithelial cells enhances early HIV transmission events. PLoS One. 2016;11(1):e0146663. (ELISA)
  9. 9. Odeh AN, Simecka JW. Regulatory CD4+CD25+ T cells dampen inflammatory disease in murine mycoplasma pneumonia and promote IL-17 and IFN-γ responses. PLoS One. 2016;11(5):e0155648. (ELISA)
  10. 10. van Ginkel FW, Gulley SL, Lammers A, Hoerr FJ, Gurjar R, Toro H. Conjunctiva-associated lymphoid tissue in avian mucosal immunity. Dev Comp Immunol. 2012;36:289-97. (ELISPOT, FC, IHC-FS)
  11. 11. Latham LB, Lee MJ, Lincoln JA, Ji N, Forsthuber TG, Lindsey JW. Antivirus immune activity in multiple sclerosis correlates with MRI activity. Acta Neurol Scand. 2016;133:17-24. (ELISPOT)
  12. 12. Bowman L, Grossmann M, Rill D, Brown M, Zhong W, Alexander B, et al. IL-2 adenovector-transduced autologous tumor cells induce antitumor immune responses in patients with neuroblastoma. Blood. 1998;92:1941-9. (FC)
  13. 13. Flynn S, Toellner K, Raykundalia C, Goodall M, Lane P. CD4 T cell cytokine differentiation: the B cell activation molecule, OX40 ligand, instructs CD4 T cells to express interleukin 4 and upregulates expression of the chemokine receptor, Blr-1. J Exp Med. 1998;188:297-304. (FC)
  14. 14. Bridle BW, Julian R, Shewen PE, Vaillancourt J, Kaushik AK. T lymphocyte subpopulations diverge in commercially raised chickens. Can J Vet Res. 2006;70:183-90. (FC)
  15. 15. Ettinger R, Munson SH, Chao C, Vadeboncoeur M, Toma J, McDevitt HO. A critical role for lymphotoxin-β receptor in the development of diabetes in nonobese diabetic mice. J Exp Med. 2001;193:1333-9. (IHC-FS)
  16. 16. Bénard A, Ceredig R, Rolink AG. Regulatory T cells control autoimmunity following syngeneic bone marrow transplantation. Eur J Immunol. 2006;36:2324-35. (IHC-FS)
  17. 17. van Ginkel FW, Tang DC, Gulley SL, Toro H. Induction of mucosal immunity in the avian Harderian gland with a replication-deficient Ad5 vector expressing avian influenza H5 hemagglutinin. Dev Comp Immunol. 2009;33:28-34. (IHC-FS)
  18. 18. Pérez CV, Pellizzari EH, Cigorraga SB, Galardo MN, Naito M, Lustig L, et al. IL17A impairs blood-testis barrier integrity and induces testicular inflammation. Cell Tissue Res. 2014;358:885-98. (IHC-FS)
  19. 19. Kivisäkk P, Mahad DJ, Callahan MK, Sikora K, Trebst C, Tucky B, et al. Expression of CCR7 in multiple sclerosis: implications for CNS immunity. Ann Neurol. 2004;55:627-38. (IHC-PS)
  20. 20. Wong CW, Wiedle G, Ballestrem C, Wehrle-Haller B, Etteldorf S, Bruckner M, et al. PECAM-1/CD31 trans-homophilic binding at the intercellular junctions is independent of its cytoplasmic domain; evidence for heterophilic interaction with integrin αvβ3 in Cis. Mol Biol Cell. 2000;11:3109-21. (ICC)
  21. 21. Sorbarzo CM, Rosana Nde M, Livia L, Berta D, Schteingart HF. Mono-(2-ethylhexyl) phthalate (MEHP) affects intercellular junctions of Sertoli cell: A potential role of oxidative stress. Reprod Toxicol. 2015;58:203-12. (ICC)
  22. 22. Clancy JP, Ruiz FE, Sorscher EJ. Adenosine and its nucleotides activate wild-type and R117H CFTR through an A2B receptor-coupled pathway. Am J Physiol. 1999;276:C361-9. (WB)
  23. 23. Bresalier RS, Byrd JC, Tessler D, Lebel J, Koomen J, Hawke D, et al. A circulating ligand for galectin-3 is a haptoglobin-related glycoprotein elevated in individuals with colon cancer. Gastroenterology. 2004;127:741-8. (WB)
  24. 24. Polyak MJ, Li H, Shariat N, Deans JP. CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins. J Biol Chem. 2008;283:18545-52. (WB)
  25. 25. Jeon M, Kang M, Park KH. 7-Triazolylcoumarin-based fluorescent tag system for stepwise, comparative assessment of small molecule microarrays. Tetrahedron. 2012;68:6038-53. (Microarray)
See All References

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