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Donkey Anti-Goat IgG Fc-HRP

Cat. No.:
6460-05
Donkey Anti-Goat IgG Fc-HRP antibody for use in ELISA assays.
$105.00
Size Price (USD) Quantity
1.0 mL $105.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey IgG
Isotype Control 0175-05
Specificity Reacts with the Fc region of goat IgG
Source Pooled antisera from donkeys hyperimmunized with goat IgG Fc
Cross Adsorption Goat IgG Fab; may react with immunoglobulins from other species
Purification Method Affinity chromatography on goat IgG Fc covalently linked to agarose
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796394

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with purified mouse IgG Fc and IgG Fab and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads-UNLB (SB Cat. No. 1013-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Mouse Anti-Goat IgG Fc-HRP (SB115d)
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  2. Normal Donkey Serum
    Normal Donkey Serum
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  3. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Human SP ads-HRP (SB Cat. No. 6026-05).
    Rabbit F(ab')2 Anti-Goat IgG(H+L), Human SP ads-HRP
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  4. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6157-05).
    Mouse Anti-Goat IgG Fc-HRP (SB115h)
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  5. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit Anti-Goat IgG(H+L)-HRP (SB Cat. No. 6160-05).
    Rabbit Anti-Goat IgG(H+L)-HRP
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  6. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit Anti-Goat IgG(H+L), Human SP ads-HRP (SB Cat. No. 6164-05).
    Rabbit Anti-Goat IgG(H+L), Human SP ads-HRP
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  7. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit F(ab')<sub>2</sub> Anti-Goat IgG(H+L)-HRP (SB Cat. No. 6020-05).
    Rabbit F(ab')2 Anti-Goat IgG(H+L)-HRP
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    $121.00
  8. ELISA plate was coated with serially diluted Goat IgG Fc-UNLB (SB Cat. No. 0130-01). Immunoglobulin was detected with Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Goat IgG Fc-UNLB
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  9. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
    Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP
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  10. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
    Goat IgG-UNLB
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  11. Paraffin embedded mouse spleen section was stained with Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP (SB Cat. No. 6420-05), DAB, and hematoxylin.
    Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP
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  12. ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Goat F(ab')2 IgG-UNLB
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  13. Purified Human Type I Collagen (SB Cat. No. 1200-01S) was resolved by electrophoresis, transferred to PVDF membrane, probed with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), and visualized using Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05) secondary antibody and chemiluminescent detection.
    Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP
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  14. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  15. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP (SB Cat. No. 6421-05).
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