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Rabbit Anti-Human IgG(H+L)-AP
Cat. No.:
6140-04
Rabbit Anti-Human IgG(H+L)-AP antibody for use in ELISA, immunocytochemistry, and western blot assays.
$116.00
| Isotype | Rabbit IgG |
|---|---|
| Isotype Control | 0111-04 |
| Specificity | Reacts with the heavy and light chains of human IgG and the light chains of human IgM and IgA |
| Source | Pooled antisera from rabbits hyperimmunized with human IgG |
| Cross Adsorption | None; may react with immunoglobulins from other species |
| Purification Method | Affinity chromatography on human IgG covalently linked to agarose |
| Conjugate | AP (Alkaline Phosphatase) |
| Buffer Formulation | 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0 |
| Clonality | Polyclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications | Quality tested applications for relevant formats include - ELISA 1-3 FLISA Other referenced applications for relevant formats include - Flow Cytometry 4 Immunocytochemistry 5 Immunoprecipitation 5 Western Blot 6 |
| RRID Number | AB_2796196 |
Documentation
Certificate of Analysis Lookup
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- 1. Yang W, Lam P, Kitching R, Kahn H, Yee A, Aubin JE, et al. Breast cancer metastasis in a human bone NOD/SCID mouse model. Cancer Biol Ther. 2007;6:1295-300. (ELISA)
- 2. Ahmad G, Zhang W, Torben W, Ahrorov A, Damian RT, Wolf RF, et al. Preclinical prophylactic efficacy testing of Sm-p80-based vaccine in a nonhuman primate model of Schistosoma mansoni infection and immunoglobulin G and E responses to Sm-p80 in human serum samples from an area where schistosomiasis is endemic. J Infect Dis. 2011;204:1437-49. (ELISA)
- 3. Luo W, Lin S, Lo K, Lu C, Hung C, Chen C, et al. Adaptive immune responses elicited by baculovirus and impacts on subsequent transgene expression in vivo. J Virol. 2013;87:4965-73. (ELISA)
- 4. Sackstein R. Directing stem cell trafficking via GPS. Methods Enzymol. 2010;479:93-105. (FC)
- 5. Knight AM, Lucocq JM, Prescott AR, Ponnambalam S, Watts C. Antigen endocytosis and presentation mediated by human membrane IgG1 in the absence of the Igα/Igβ dimer. EMBO J. 1997;16:3842-50. (ICC, IP)
- 6. Di Zenzo G, Calabresi V, Grosso F, Caproni M, Ruffelli M, Zambruno G. The intracellular and extracellular domains of BP180 antigen comprise novel epitopes targeted by pemphigoid gestationis autoantibodies. J Invest Dermatol. 2007;127:864-73. (WB)
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