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Goat Anti-Rabbit IgM-AP

Goat Anti-Rabbit IgM-AP

Name: Goat Anti-Rabbit IgM-AP
SKU: 4020-04
Price: 132 USD
Availability: InStock

Cat. No.:

4020-04

Goat Anti-Rabbit IgM-AP antibody for use in ELISA, immunohistochemistry, western blot, and ELISpot assays.

$132.00

Size	Price (USD)	Quantity
1.0 mL	$132.00

Qty

Technical Bulletin Safety Datasheet

ELISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-AP (SB Cat. No. 4020-04).

Product Specifications

More Information
Isotype	Goat IgG
Isotype Control	0109-04
Specificity	Reacts with the heavy chain of rabbit IgM
Source	Pooled antisera from goats hyperimmunized with rabbit IgM
Cross Adsorption	Rabbit IgG; may react with immunoglobulins from other species
Purification Method	Affinity chromatography on pooled rabbit IgM covalently linked to agarose
Conjugate	AP (Alkaline Phosphatase)
Buffer Formulation	50 mM Tris/1 mM MgCl₂/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Clonality	Polyclonal
Concentration	Lot specific
Volume	1.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1-5 FLISA Other referenced applications for relevant formats include - Flow Cytometry ^6-13 ELISPOT ^3,13 Immunohistochemistry-Frozen Sections ^14,15 Immunohistochemistry-Paraffin Sections ¹⁶ Western Blot ^17-20 Separation ^10,21
RRID Number	AB_2795927

Documentation

Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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Other Conjugates and Formulations

Citations

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8. Yang G, Obiakor H, Sinha RK, Newman BA, Hood BL, Conrads TP, et al. Activation-induced deaminase cloning, localization, and protein extraction from young V_H-mutant rabbit appendix. Proc Natl Acad Sci USA. 2005;102:17083-8. (FC)
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10. Yang J, Pospisil R, Mage RG. Expression and localization of rabbit B-cell activating factor (BAFF) and its specific receptor BR3 in cells and tissues of the rabbit immune system. Dev Comp Immunol. 2009;33:697-708. (FC, Sep)
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14. Schiaffella E, Sehgal D, Anderson AO, Mage RG. Gene conversion and hypermutation during diversification of V_H sequences in developing splenic germinal centers of immunized rabbits. J Immunol. 1999;162:3984-95. (IHC-FS)
15. Sehgal D, Obiakor H, Mage RG. Distinct clonal Ig diversification patterns in young appendix compared to antigen-specific splenic clones. J Immunol. 2002;168:5424-33. (IHC-FS)
16. Rosner A, Moiseeva E, Rabinowitz C, Rinkevich B. Germ lineage properties in the urochordate Botryllus schlosseri – From markers to temporal niches. Dev Biol. 2013;384:356-74. (IHC-PS)
17. Gibson JR, Chart H, Owen RJ. Intra-strain variation in expression of lipopolysaccharide by Helicobacter pylori. Lett Appl Microbiol. 1998;26:399-403. (WB)
18. Chart H, Cheasty T, Willshaw GA. Production of serum antibodies that recognise epitopes located on the R3 region of Escherichia coli core lipopolysaccharides by patients infected with strains of enterohaemorrhagic E. coli. J Med Microbiol. 2002;51:1050-4. (WB)
19. Chart H, Cheasty T, de Pinna E, Siovanes L, Wain J, Alam D, et al. Serodiagnosis of Salmonella enterica serovar Typhi and S. enterica serovars Paratyphi A, B and C human infections. J Med Microbiol. 2007;56:1161-6. (WB)
20. Furuya K, Omura M, Kudo S, Sugiura W, Azuma H. Recognition profiles of microsporidian Encephalitozoon cuniculi polar tube protein 1 with human immunoglobulin M antibodies. Parasite Immunol. 2008;30:13-21. (WB)
21. Pospisil R, Fitts MG, Mage RG. CD5 is a potential selecting ligand for B cell surface immunoglobulin framework region sequences. J Exp Med. 1996;184:1279-84. (Sep)