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Goat Anti-Human IgG(H+L), Mouse/Bovine/Horse SP ads-BIOT

Cat. No.:
2016-08
Goat Anti-Human IgG(H+L)-Biotin antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in ELISA assays.
$138.00
Size Price (USD) Quantity
1.0 mg $138.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy and light chains of human IgG and the light chains of human IgM and IgA
Source Pooled antisera from goats hyperimmunized with human IgG
Cross Adsorption Mouse, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on human IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2795596

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat IgG-BIOT (SB Cat No. 0109-08; gray) and Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
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  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  3. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 2087-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  4. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Human IgG(H+L)-BIOT (SB Cat. No. 6140-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Rabbit Anti-Human IgG(H+L)-BIOT
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  5. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
    Human IgG-UNLB
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  6. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG(H+L)-BIOT (SB Cat. No. 2015-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  7. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  8. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  9. ELISA plate was coated with purified human IgG, IgM, and IgA and mouse, bovine, and horse serum. Immunoglobulins and sera were detected with Rabbit Anti-Human IgG(H+L), Mouse/Bovine/Horse SP ads-UNLB (SB Cat. No. 6146-01) followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05).
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  10. ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM-BIOT (SB Cat. No. 2020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  11. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
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  12. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Human IgG(H+L), Mouse ads-BIOT (SB Cat. No. 6145-08) followed by Strepatvidin-HRP (SB Cat. No. 7100-05).
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  13. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  14. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Lambda-UNLB (SB Cat. No. 0151L-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgG1 Lambda-UNLB
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  15. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  16. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).
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  17. ELISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Human IgA-BIOT (SB Cat. No. 2050-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  18. ELISA plate was coated with serially diluted Human IgG Fc-UNLB (SB Cat. No. 0160-01). Immunoglobulin was detected with Goat Anti-Human IgG Fc-BIOT (SB Cat. No. 2048-08), Goat Anti-Human Ig Fab-BIOT (SB Cat. No. 2085-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT and Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9130-08 and Cat. No. 9140-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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