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Rat Anti-Mouse CD49e-AF488 (5H10.27)
Cat. No.:
1695-30
Alexa Fluor® 488 Anti-Mouse CD49e antibody for use in flow cytometry and immunohistochemistry assays.
$219.00
| Clone | 5H10.27 |
|---|---|
| Isotype | Rat (Lewis) IgG2aκ |
| Isotype Control | Rat IgG2a-AF488 (KLH/G2a-1-1) |
| Specificity | Mouse CD49e |
| Alternative Names | Integrin α5, VLA-5α |
| Description | The monoclonal antibody 5H10.27 reacts with the α5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines. Soluble 5H10.27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10.27 has also been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody. |
| Immunogen | C57BL/6 x A/J F1 mouse mast cell line MC/9 |
| Conjugate | AF488 (Alexa Fluor® 488) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Flow Cytometry – Quality tested 1,2 Immunohistochemistry-Frozen Sections – Reported in literature 3 Blocking – Reported in literature 1,2 Costimulation – Reported in literature 2 Adhesion – Reported in literature 1,2 |
| RRID Number | AB_2795149 |
| Gene ID | 16402 (Mouse) |
| Gene ID Symbol | Itga5 (Mouse) |
| Gene ID Aliases | Fnra; VLA5; Cd49e |
| UniProt ID | P11688 (Mouse |
| UniProt Name | ITA5_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994;20:25-44. (Immunogen, FC, Block, Adhesion)
- 2. Rich S, Van Nood N, Lee H. Role of α5β1 integrin in TGF-β1-costimulated CD8+ T cell growth and apoptosis. J Immunol. 1996;157:2916-23. (Costim, Block, Adhesion, FC)
- 3. Schmieder AH, Caruthers SD, Zhang H, Williams TA, Robertson JD, Wickline SA, et al. Three-dimensional MR mapping of angiogenesis with α5β1(αVβ3)-targeted theranostic nanoparticles in the MDA-MB-435 xenograft mouse model. FASEB J. 2008;22:4179-89. (IHC-FS)
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