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Rat Anti-Mouse CD45-LE/AF (I3/2.3)
Cat. No.:
1660-14
Low Endotoxin/Azide Free Anti-Mouse CD45 antibody for use in flow cytometry, immunohistochemistry, and immunoprecipitation assays.
$275.00
| Clone | I3/2.3 |
|---|---|
| Isotype | Rat (Lewis) IgG2bλ |
| Isotype Control | Rat IgG2b-LE/AF (KLH/G2b-1-2) |
| Specificity | Mouse CD45 |
| Alternative Names | LCA, leukocyte common antigen, T200, Ly-5, Receptor-type tyrosine-protein phosphatase C, Lyt-4, Ptprc |
| Description | CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes. CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The I3/2.3 monoclonal antibody recognizes a framework epitope present on all CD45 isoforms. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells. |
| Immunogen | T1M1 (Thy-1─c) cells |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications | Flow Cytometry – Quality tested 2,5-7 Immunohistochemistry-Frozen Sections – Reported in literature 3,4 Immunoprecipitation – Reported in literature 1,2 |
| RRID Number | AB_2795104 |
| Gene ID | 19264 (Mouse) |
| Gene ID Symbol | Ptprc (Mouse) |
| Gene ID Aliases | loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4 |
| UniProt ID | P06800 (Mouse |
| UniProt Name | PTPRC_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Trowbridge IS. Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200. J Exp Med. 1978;148:313-23. (Immunogen, IP)
- 2. Haidl ID, Ng DH, Rothenberger S, Johnson P, Jefferies WA. Detection of restricted isoform expression and tyrosine phosphatase activity of CD45 in murine dendritic cells. Eur J Immunol. 1995;25:3370-4. (FC, IP)
- 3. Craner MJ, Damarjian TG, Liu S, Hains BC, Lo AC, Black JA, et al. Sodium channels contribute to microglia/macrophage activation and function in EAE and MS. Glia. 2005;49:220-9. (IHC-FS)
- 4. Villalta SA, Lang J, Kubeck S, Kabre B, Szot GL, Calderon B, et al. Inhibition of VEGFR-2 reverses type 1 diabetes in NOD mice by abrogating insulitis and restoring islet function. Diabetes. 2013;62:2870-8. (IHC-FS)
- 5. Takedachi M, Qu D, Ebisuno Y, Oohara H, Joachims ML, McGee ST, et al. CD73-generated adenosine restricts lymphocyte migration into draining lymph nodes. J Immunol. 2008;180:6288-96. (FC)
- 6. Matsunaga H, Hokari R, Kurihara C, Okada Y, Takebayashi K, Okudaira K, et al. Omega-3 fatty acids exacerbate DSS-induced colitis through decreased adiponectin in colonic subepithelial myofibroblasts. Inflamm Bowel Dis. 2008;14:1348-57. (FC)
- 7. Domingos-Pereira S, Decrausaz L, Derré L, Bobst M, Romero P, Schiller JT, et al. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice. Mucosal Immunol. 2013;6:393-404. (FC)
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