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Mouse Anti-Phosphotyrosine-AP (PY20)
Cat. No.:
1400-04
AP Anti-Phosphotyrosine antibody for use in ELISA, immunohistochemistry / immunocytochemistry, and western blot assays.
$281.00
| Clone | PY20 |
|---|---|
| Isotype | Mouse (BALB/c) IgG2bκ |
| Isotype Control | Mouse IgG2b-AP (A-1) |
| Specificity | Tyrosine-phosphorylated proteins |
| Alternative Names | pTyr |
| Description | Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate. |
| Immunogen | Phosphotyrosine conjugated to carrier proteins |
| Conjugate | AP (Alkaline Phosphatase) |
| Buffer Formulation | 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0 |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications |
ELISA – Quality tested 1-4 FLISA – Quality tested Western Blot – Reported in literature 1,5-7 Immunoprecipitation – Reported in literature 5 Immunohistochemistry-Paraffin Sections – Reported in literature 8 Immunohistochemsitry-Whole Mount – Reported in literature 9 Immunocytochemistry – Reported in literature 10 Flow Cytometry – Reported in literature 11 Microarray – Reported in literature 12,13 Purification – Reported in literature 1,14 |
| RRID Number | AB_2794750 |
Documentation
Certificate of Analysis Lookup
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- 1. Glenney JR Jr, Zokas L, Kamps MP. Monoclonal antibodies to phosphotyrosine. J Immunol Methods. 1988;109:277-85. (Immunogen, ELISA, WB, Purification)
- 2. Ruff-Jamison S, Campos-González R, Glenney JR Jr. Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features. J Biol Chem. 1991;266:6607-13. (ELISA)
- 3. Lietha D, Eck MJ. Crystal structures of the FAK kinase in complex with TAE226 and related bis-anilino pyrimidine inhibitors reveal a helical DFG conformation. PLoS One. 2008;3(11):e3800. (ELISA)
- 4. Antila H, Autio H, Turunen L, Harju K, Tammela P, Wennerberg K, et al. Utilization of in situ ELISA method for examining Trk receptor phosphorylation in cultured cells. J Neurosci Methods. 2014;222:142-6. (ELISA)
- 5. Vouri K, Ruoslahti E. Tyrosine phosphorylation of p130Cas and cortactin accompanies integrin-mediated cell adhesion to extracellular matrix. J Biol Chem. 1995;270:22259-62. (WB, IP)
- 6. Elliott S, Egrie J, Browne J, Lorenzini T, Busse L, Rogers N, et al. Control of rHuEPO biological activity: the role of carbohydrate. Exp Hematol. 2004;32:1146-55. (WB)
- 7. Wang X, Cahill ME, Werner CT, Christoffel DJ, Golden SA, Xie Z, et al. Kalirin-7 mediates cocaine-induced AMPA receptor and spine plasticity, enabling incentive sensitization. J Neurosci. 2013;33:11012-22. (WB)
- 8. Haralambieva E, Jones M, Roncador GM, Cerroni L, Lamant L, Ott G, et al. Tyrosine phosphorylation in human lymphomas. Histochem J. 2002;34:545-52. (IHC-PS)
- 9. Gorczyca M, Augart C, Budnik V. Insulin-like receptor and insulin-like peptide are localized at neuromuscular junctions in Drosophila. J Neurosci. 1993;13:3692-704. (IHC-WM)
- 10. Takagi S, Daibata M, Last TJ, Humphreys RE, Parker DC, Sairenji T. Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells. J Exp Med. 1991;174:381-8. (ICC)
- 11. Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000;39:250-9. (FC)
- 12. Sikkema AH, de Bont ES, Molema G, Dimberg A, Zwiers PJ, Diks SH, et al. Vascular endothelial growth factor receptor 2 (VEGFR-2) signalling activity in paediatric pilocytic astrocytoma is restricted to tumour endothelial cells. Neuropathol Appl Neurobiol. 2011;37:538-48. (Microarray)
- 13. Rosenberger AF, Hilhorst R, Coart E, GarcÃa Barrado L, Naji F, Rozemuller AJ, et al. Protein kinase activity decreases with higher braak stages of alzheimer's disease pathology. J Alzheimers Dis. 2016;49:927-43. (Microarray)
- 14. Glenney JR Jr, Zokas L. Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton. J Cell Biol. 1989;108:2401-8. (Purification)
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