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  • Goat Anti-Type VI Collagen-UNLB

Goat Anti-Type VI Collagen-UNLB

Cat. No.:
1360-01
Purified Anti-Type VI Collagen antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, and western blot assays.
$435.00
Size Price (USD) Quantity
0.2 mg $435.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Specificity Reacts with conformational determinants on type VI collagen
Source Pooled antisera from goats hyperimmunized with type VI collagen
Cross Adsorption Collagen types I, II, III, IV, and V
Purification Method Affinity chromatography on type VI collagen covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 0.4 mg/mL
Volume 0.5 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1

Other referenced applications for relevant formats include -
Immunohistochemistry-Paraffin Sections 2-15
Immunohistochemistry-Frozen Sections 7,16,17
Immunocytochemistry 7,18
Electron Microscopy 19
Western Blot 20
RRID Number AB_2721908
Gene ID 1291 (Human)
1292 (Human)
1293 (Human)
Gene ID Symbol COL6A1 (Human)
COL6A2 (Human)
COL6A3 (Human)
Gene ID Aliases OPLL; BTHLM1; UCHMD1; UCMD1; PP3610; DYT27
UniProt ID P12109 (Human
P12110 (Human
P12111 (Human
UniProt Name CO6A1_HUMAN (Human)
CO6A2_HUMAN (Human)
CO6A3_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Rasmussen LM, Hansen PR, Ledet T. Homocysteine and the production of collagens, proliferation and apoptosis in human arterial smooth muscle cells. APMIS. 2004;112:598-604. (ELISA, Human Reactivity)
  2. 2. Horie K, Miyata T, Maeda K, Miyata S, Sugiyama S, Sakai H, et al. Immunohistochemical colocalization of glycoxidation products and lipid peroxidation products in diabetic renal glomerular lesions. Implication for glycoxidative stress in the pathogenesis of diabetic nephropathy. J Clin Invest. 1997;100:2995-3004. (IHC-PS, Human Reactivity)
  3. 3. Tsutsumi Y, Tazawa K, Shibuya M. Type VI collagen immunoreactivity in skeinoid fibers in small intestinal stromal tumors. Pathol Int. 1999;49:836-9. (IHC-PS, Human Reactivity)
  4. 4. White SA, Hughes DP, Contractor HH, London NJ. An investigation into the distribution of different collagen types within adult and juvenile porcine pancreata. J Mol Med. 1999;77:79-82. (IHC-PS, Porcine Reactivity)
  5. 5. Shigemitsu T, Ishiguro K, Shimizu Y, Horiguchi M, Kasahara M, Arakaki S. Immunocytochemical features of lens after cataract tissue – signalling molecules (growth factors, cytokines, other signalling molecules), cytoskeleton proteins, cellular and extracellular matrix proteins. Int Ophthalmol. 2001;23:137-44. (IHC-PS, Human Reactivity)
  6. 6. Saika S, Yamanaka O, Baba Y, Kawashima Y, Shirai K, Miyamoto T, et al. Accumulation of latent transforming growth factor-β binding protein-1 and TGFβ1 in extracellular matrix of filtering bleb and of cultured human subconjunctival fibroblasts. Graefes Arch Clin Exp Ophthalmol. 2001;239:234-41. (IHC-PS, Human Reactivity)
  7. 7. Ishida I, Saika S, Ohnishi Y. Effect of minoxidil on rabbit lens epithelial cell behavior in vitro and in situ. Graefes Arch Clin Exp Ophthalmol. 2001;239:770-7. (IHC-PS, IHC-FS, ICC, Rabbit Reactivity)
  8. 8. Palmans E, Pauwels RA, Kips JC. Repeated allergen exposure changes collagen composition in airways of sensitised Brown Norway rats. Eur Respir J. 2002;20:280-5. (IHC-PS, Rat Reactivity)
  9. 9. Saika S, Miyamoto T, Tanaka T, Ohnishi Y, Ooshima A, Kimura W. Histopathology of anterior lens capsules in vitrectomized eyes with tamponade by silicone oil. J Cataract Refract Surg. 2002;28:376-8. (IHC-PS, Human Reactivity)
  10. 10. Gittenberger-de Groot AC, Bartram U, Oosthoek PW, Bartelings MM, Hogers B, Poelmann RE, et al. Collagen type VI expression during cardiac development and in human fetuses with trisomy 21. Anat Rec A Discov Mol Cell Evol Biol. 2003;275:1109-16. (IHC-PS, Human Reactivity)
  11. 11. Saika S, Miyamoto T, Ishida I, Barbour WK, Ohnishi Y, Ooshima A. Accumulation of thrombospondin-1 in post-operative capsular fibrosis and its down-regulation in lens cells during lens fiber formation. Exp Eye Res. 2004;79:147-56. (IHC-PS, Human Reactivity)
  12. 12. Schaefer L, Mihalik D, Babelova A, Krzyzankova M, Gröne H, Iozzo RV, et al. Regulation of fibrillin-1 by biglycan and decorin is important for tissue preservation in the kidney during pressure-induced injury. Am J Pathol. 2004;165:383-96. (IHC-PS, Mouse Reactivity)
  13. 13. Ishida I, Saika S, Okada Y, Ohnishi Y. Growth factor deposition in anterior subcapsular cataract. J Cataract Refract Surg. 2005;31:1219-25. (IHC-PS, Human Reactivity)
  14. 14. Pecorella I, Taloni M, Ciardi A, Alexander RA, Falcinelli G. Osteo-odonto-keratoprosthesis: a human model of autotransplant. Curr Eye Res. 2006;31:835-43. (IHC-PS, Human Reactivity)
  15. 15. Kruithof BP, Krawitz SA, Gaussin V. Atrioventricular valve development during late embryonic and postnatal stages involves condensation and extracellular matrix remodeling. Dev Biol. 2007;302:208-17. (IHC-PS, Mouse Reactivity)
  16. 16. Jeffery G, Evans A, Albon J, Duance V, Neal J, Dawidek G. The human optic nerve: fascicular organisation and connective tissue types along the extra-fascicular matrix. Anat Embryol. 1995;191:491-502. (IHC-FS, Human Reactivity)
  17. 17. Fraser SA, Crawford A, Frazer A, Dickinson S, Hollander AP, Brook IM, et al. Localization of type VI collagen in tissue-engineered cartilage on polymer scaffolds. Tissue Eng. 2006;12:569-77. (IHC-FS, Bovine & Human Reactivity)
  18. 18. Hsieh-Bonassera ND, Wu I, Lin JK, Schumacher BL, Chen AC, Masuda K, et al. Expansion and redifferentiation of chondrocytes from osteoarthritic cartilage: cells for human cartilage tissue engineering. Tissue Eng Part A. 2009;15:3513-23. (ICC, Human Reactivity)
  19. 19. Dingemans KP, Teeling P, Lagendijk JH, Becker AE. Extracellular matrix of the human aortic media: an ultrastructural histochemical and immunohistochemical study of the adult aortic media. Anat Rec. 2000;258:1-14. (EM, Human Reactivity)
  20. 20. Bashey RI, Martinez-Hernandez A, Jimenez SA. Isolation, characterization, and localization of cardiac collagen type VI. Associations with other extracellular matrix components. Circ Res. 1992;70:1006-17. (WB, Canine, Cynomolgus, & Rat Reactivity)
See All References

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  2. Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF488 (SB Cat. No. 6420-30) and DAPI.
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  3. ELISA plate was coated with serially diluted Bovine Type VI Collagen (SB Cat. No. 1300-02). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  4. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6157-05).
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  5. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
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  6. ELISA plate was coated with serially diluted Bovine Type VI Collagen (SB Cat. No. 1300-02S). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  7. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF647 (SB Cat. No. 6425-31).
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