My Cart
$0.00
0

Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

  • Home
  • Rat Anti-Mouse IgE-UNLB (23G3)

Rat Anti-Mouse IgE-UNLB (23G3)

Cat. No.:
1130-01
Purified Anti-Mouse IgE antibody for use in ELISA, flow cytometry, immunohistochemistry, western blot, and ELISpot assays.
$204.00
Size Price (USD) Quantity
0.5 mg $204.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone 23G3
Isotype Rat IgG1κ
Isotype Control Rat IgG1-UNLB (KLH/G1-2-2)
Specificity Mouse IgE
Immunogen Monoclonal anti-DNP mouse IgE
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested 1-11
FLISA – Quality tested
Flow Cytometry – Reported in literature 12-16
Immunohistochemistry-Frozen Sections – Reported in literature 17,18
Immunohistochemistry-Paraffin Sections – Reported in literature 23
Immunohistochemistry-Whole Mount – Reported in literature 19
Western Blot – Reported in literature 5,21
ELISpot – Reported in literature 1,20
Neutralization – Reported in literature 22

RRID Number AB_2794615
Gene ID 380792 (Mouse)
Gene ID Symbol Ighe (Mouse)
Gene ID Aliases Gm900
UniProt ID P06336 (Mouse
UniProt Name IGHE_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

Citations

  1. 1. Petit-Frere C, Dugas B, Braquet P, Mencia-Huerta JM. Interleukin-9 potentiates the interleukin-4-induced IgE and IgG1 release from murine B lymphocytes. Immunology. 1993;79:146-51. (ELISA, ELISPOT)
  2. 2. Takahashi I, Marinaro M, Kiyono H, Jackson RJ, Nakagawa I, Fujihashi K, et al. Mechanisms for mucosal immunogenicity and adjuvancy of Escherichia coli labile enterotoxin. J Infect Dis. 1996;173:627-35. (ELISA)
  3. 3. Kasprowicz DJ, Kohm AP, Berton MT, Chruscinski AJ, Sharpe A, Sanders VM, et al. Stimulation of the B cell receptor, CD86 (B7-2), and the β2-adrenergic receptor intrinsically modulates the level of IgG1 and IgE produced per B cell. J Immunol. 2000;165:680-90. (ELISA)
  4. 4. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ELISA)
  5. 5. Lehrer SB, Reish R, Fernandes J, Gaudry P, Dai G, Reese G. Enhancement of murine IgE antibody detection by IgG removal. J Immunol Methods. 2004;284:1-6. (ELISA, WB)
  6. 6. Ramalingam TR, Pesce JT, Sheikh F, Cheever AW, Mentink-Kane MM, Wilson MS, et al. Unique functions of the type II interleukin 4 receptor identified in mice lacking the interleukin 13 receptor α1 chain. Nat Immunol. 2008;9:25-33. (ELISA)
  7. 7. Li F, Wang L, Jin X, Yan C, Jiang S, Shen X. The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice. Immunobiology. 2009;214:87-99. (ELISA)
  8. 8. Kamphuis T, Meijerhof T, Stegmann T, Lederhofer J, Wilschut J, de Haan A. Immunogenicity and protective capacity of a virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice. PLoS One. 2012;7(5):e36812. (ELISA)
  9. 9. Futatsugi-Yumikura S, Matsushita K, Fukuoka A, Takahashi S, Yamamoto N, Yonehara S, et al. Pathogenic Th2-type follicular helper T cells contribute to the development of lupus in Fas-deficient mice. Int Immunol. 2014;26:221-31. (ELISA)
  10. 10. Stefka AT, Feehley T, Tripathi P, Qiu J, McCoy K, Mazmanian SK, et al. Commensal bacteria protect against food allergen sensitization. Proc Natl Acad Sci USA. 2014;111:13145-50. (ELISA)
  11. 11. Pucella JN, Yen W, Kim MV, van der Veeken J, Socci ND, Naito Y, et al. miR-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function. J Immunol. 2015;194:2635-42. (ELISA)
  12. 12. Kuzin II, Snyder JE, Ugine GD, Wu D, Lee S, Bushnell T Jr, et al. Tetracyclines inhibit activated B cell function. Int Immunol. 2001;12:921-31. (FC)
  13. 13. Huber M, Kalis C, Keck S, Jiang Z, Georgel P, Du X, et al. R-form LPS, the master key to the activation of TLR4/MD-2-positive cells. Eur J Immunol. 2006;36:701-11. (FC)
  14. 14. Seidl A, Panzer M, Voehringer D. Protective immunity against the gastrointestinal nematode Nippostrongylus brasiliensis requires a broad T-cell receptor repertoire. Immunology. 2011;134:214-23. (FC)
  15. 15. Kamijo S, Takeda H, Tokura T, Suzuki M, Inui K, Hara M, et al. IL-33-mediated innate response and adaptive immune cells contribute to maximum responses of protease allergen-induced allergic airway inflammation. J Immunol. 2013;190:4489-99. (FC)
  16. 16. Muto T, Fukuoka A, Kabashima K, Ziegler SF, Nakanishi K, Matsushita K, et al. The role of basophils and proallergic cytokines, TSLP and IL-33, in cutaneously sensitized food allergy. Int Immunol. 2014;26:539-49. (FC)
  17. 17. Brown JK, Knight PA, Pemberton AD, Wright SH, Pate JA, Thronton EM, et al. Expression of integrin-αE by mucosal mast cells in the intestinal epithelium and its absence in nematode-infected mice lacking the transforming growth factor-β1-activating integrin αvβ6. Am J Pathol. 2004;165:95-106. (IHC-FS)
  18. 18. Inman CF, Rees LE, Barker E, Haverson K, Stokes CR, Bailey M. Validation of computer-assisted, pixel-based analysis of multiple-colour immunofluorescence histology. J Immunol Methods. 2005;302:156-67. (IHC-FS)
  19. 19. Pochanke V, Koller S, Dayer R, Hatak S, Ludewig B, Zinkernagel RM, et al. Identification and characterization of a novel antigen from the nematode Nippostrongylus brasiliensis recognized by specific IgE. Eur J Immunol. 2007;37:1275-84. (IHC-WM)
  20. 20. Padro CJ, Shawler TM, Gormley MG, Sanders VM. Adrenergic regulation of IgE involves modulation of CD23 and ADAM10 expression on exosomes. J Immunol. 2013;191:5383-97. (ELISPOT)
  21. 21. Ambrosi G, Ghezzi C, Sepe S, Milanese C, Payan-Gomez C, Bombardieri CR, et al. Bioenergetic and proteolytic defects in fibroblasts from patients with sporadic Parkinson's disease. Biochim Biophys Acta. 2014;1842:1385-94. (WB)
  22. 22. Dakhama A, Lee Y, Ohnishi H, Jing X, Balhorn A, Takeda K, et al. Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice. J Allergy Clin Immunol. 2009;123:138-45. (Neut)
  23. 23. SouthernBiotech published data (IHC-PS)
See All References

Related Products

Related Products

Related Products
Check items to add to the cart or
  1. ELISA plate was coated with serially diluted Rat IgG<sub>1</sub>-UNLB (SB Cat. No. 0116-01). Immunoglobulin was detected with Mouse Anti-Rat IgG<sub>1</sub>-BIOT (SB Cat. No. 3061-08), Mouse Anti-Rat IgG<sub>2a</sub>-BIOT (SB Cat. No. 3065-08), Mouse Anti-Rat IgG<sub>2b</sub>-BIMA (SB Cat. No. 3070-28), Mouse Anti-Rat IgG<sub>2c</sub>-BIOT (SB Cat. No. 3075-08), and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Rat IgG1-UNLB (KLH/G1-2-2)
    0116-01
    $130.00
  2. Paraffin embedded mouse small intestine section was stained with Rat Anti-Mouse IgD-UNLB (SB Cat. No. 1120-01; right) followed by an HRP conjugated secondary antibody, DAB, and hematoxylin.
    Rat Anti-Mouse IgD-UNLB (11-26)
    1120-01
    $182.00
  3. ELISA plate was coated with serially diluted Mouse IgE-UNLB (SB Cat. No. 0114-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08), and Goat Anti-Mouse IgE-BIOT (SB Cat. No. 1110-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Mouse IgE-UNLB (15.3)
    0114-01
    $165.00
  4. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD22-UNLB (SB Cat. No. 1580-01) followed by Mouse Anti-Rat IgG<sub>1</sub>-AF488 (SB Cat. No. 3061-30).
    Mouse Anti-Rat IgG1-AF488 (G11C5)
    3061-30
    $289.00
  5. ELISA plate was coated with purified mouse IgM, IgG, and IgA and human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Mouse IgM, Human ads-UNLB
    1020-01
    $127.00
  6. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
    0100-01
    $45.00
  7. FLISA plate was coated with purified rat IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG<sub>1</sub>-CY5 (SB Cat. No. 3060-15).
    Mouse Anti-Rat IgG1-CY5 (G17E7)
    3060-15
    $330.00
  8. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-UNLB (SB Cat. No. 1070-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat Anti-Mouse IgG1, Human ads-UNLB
    1070-01
    $198.00
  9. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-UNLB (SB Cat. No. 1080-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Mouse IgG2a, Human ads-UNLB
    1080-01
    $198.00
  10. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD4-UNLB (SB Cat. 1540-01) followed by Goat Anti-Rat IgG(H+L), Mouse ads-FITC (SB Cat. No. 3050-02).
    Goat Anti-Rat IgG(H+L), Mouse ads-FITC
    3050-02
    $182.00
  11. ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgM-UNLB (SB Cat. No. 1140-01) followed by Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05).
    Rat Anti-Mouse IgM-UNLB (1B4B1)
    1140-01
    $176.00
  12. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-SPRD (SB Cat. No. 3052-13).
    Goat F(ab')2 Anti-Rat IgG(H+L), Mouse ads-SPRD
    3052-13
    $297.00
  13. Paraffin embedded BALB/c mouse colon tissue section was stained with Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-FITC (SB Cat. No. 6420-02) and DAPI.
    Goat Anti-Mouse IgA-UNLB
    1040-01
    $116.00