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Goat Anti-Mouse IgG2b, Human ads-HRP

Goat Anti-Mouse IgG2b, Human ads-HRP

Name: Goat Anti-Mouse IgG2b, Human ads-HRP
SKU: 1090-05
Price: 146 USD
Availability: InStock

Cat. No.:

1090-05

Goat Anti-Mouse IgG_2b-HRP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blot, and ELISpot assays.

$146.00

Size	Price (USD)	Quantity
1.0 mL	$146.00

Qty

Technical Bulletin Safety Datasheet

ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-HRP (SB Cat. No. 1090-05).

ELISA plate was coated with purified mouse IgG₁, IgG_2a, IgG_2b, IgG₃, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG_2b, Human ads-HRP (SB Cat. No. 1090-05).

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01) followed by Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G<sup>&re

Lane 1 - Untreated Jurkat whole cell lysate
Lane 2 - TSA-treated Jurkat whole cell lysate (400 nM, 12 hr)
Cell lysates were resolved by electrophoresis, transferred to PVDF membrane, probed with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; IgG<sub>2a</sub>) and Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01; IgG<sub>2b</sub>) followed by Goat Anti-Mouse IgG<sub>2

Product Specifications

More Information
Isotype	Goat IgG
Isotype Control	0109-05
Specificity	Reacts with the heavy chain of mouse IgG_2b
Source	Pooled antisera from goats hyperimmunized with mouse IgG_2b
Cross Adsorption	Mouse IgG₁, IgG_2a, IgG₃, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method	Affinity chromatography on mouse IgG_2b covalently linked to agarose
Conjugate	HRP (Horseradish Peroxidase)
Buffer Formulation	50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality	Polyclonal
Concentration	Lot specific
Volume	1.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1-12 FLISA Flow Cytometry ^13-15 Other referenced applications for relevant formats include - ELISpot ^4,6,16 Immunohistochemistry-Frozen Sections ^17-19 Immunohistochemistry-Paraffin Sections ^17,20 Immunocytochemistry ^21,22 Western Blot ^23,24
RRID Number	AB_2794521
Gene ID	16016 (Mouse)
Gene ID Symbol	Ighg2b (Mouse)
Gene ID Aliases	IgG2b; Igh-3; gamma2b
UniProt ID	P01867 (Mouse
UniProt Name	IGG2B_MOUSE (Mouse)

Documentation

Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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Citations

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8. Aguilar JL, Varshney AK, Wang X, Stanford L, Scharff M, Fries BC. Detection and measurement of staphylococcal enterotoxin-like K (SEl-K) secretion by Staphylococcus aureus clinical isolates. J Clin Microbiol. 2014;52:2536-43. (ELISA)
9. Shen P, Roch T, Lampropoulou V, O'Connor RA, Stervbo U, Hilgenberg E, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases. Nature. 2014;507:366-70. (ELISA)
10. Quakkelaar ED, Fransen MF, van Maren WW, Vaneman J, Loof NM, van Heiningen SH, et al. IgG-mediated anaphylaxis to a synthetic long peptide vaccine containing a B cell epitope can be avoided by slow-release formulation. J Immunol. 2014;192:5813-20. (ELISA)
11. Patel JM, Vartabedian VF, Kim M, He S, Kang S, Selvaraj P. Influenza virus-like particles engineered by protein transfer with tumor-associated antigens induces protective antitumor immunity. Biotechnol Bioeng. 2015;112:1102-10. (ELISA)
12. Hartung E, Becker M, Bachem A, Reeg N, Jäkel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79. (ELISA)
13. Gliddon DR, Howard CJ. CD26 is expressed on a restricted subpopulation of dendritic cells in vivo. Eur J Immunol. 2002;32:1472-81. (FC)
14. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (FC)
15. Klausmann S, Sydler T, Summerfield A, Lewis FI, Weilenmann R, Sidler X, et al. T-cell reprogramming through targeted CD4-coreceptor and T-cell receptor expression on maturing thymocytes by latent Circoviridae family member porcine circovirus type 2 cell infections in the thymus. Emerg Microbes Infect. 2015;4:e15. (FC)
16. Hsu H, Wu Y, Yang P, Wu Q, Job G, Chen J, et al. Overexpression of activation-induced cytidine deaminase in B cells is associated with production of highly pathogenic autoantibodies. J Immunol. 2007;178:5357-65. (ELISPOT)
17. van der Loos C. User Protocol: Practical Guide to Multiple Staining. BioTechniques' Protocol Guide 2010. 2009 Nov. doi: doi10.2144/000113281. (IHC-FS, IHC-PS)
18. Popa ER, Harmsen MC, Tio RA, van der Strate BW, Brouwer LA, Schipper M, et al. Circulating CD34⁺ progenitor cells modulate host angiogenesis and inflammation in vivo. J Mol Cell Cardiol. 2006;41:86-96. (IHC-FS)
19. Jacob N, Guo S, Mathian A, Koss MN, Gindea S, Putterman C, et al. B cell and BAFF dependence of IFN-α-exaggerated disease in systemic lupus erythematosus-prone NZM 2328 mice. J Immunol. 2011;186:4984-93. (IHC-FS)
20. Huizinga R, van der Star BJ, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31. (IHC-PS)
21. Hanon E, Stinchcombe JC, Saito M, Asquith BE, Taylor GP, Tanaka Y, et al. Fratricide among CD8⁺ T lymphocytes naturally infected with human T cell lymphotropic virus type I. Immunity. 2000;13:657-64. (ICC)
22. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
23. Elkabetz Y, Argon Y, Bar-Nun S. Cysteines in C_H1 underlie retention of unassembled Ig heavy chains. J Biol Chem. 2005;280:14402-12. (WB)
24. Schott C, Graab U, Cuvelier N, Hahn H, Fulda S. Oncogenic RAS mutants confer resistance of RMS13 rhabdomyosarcoma cells to oxidative stress-induced ferroptotic cell death. Front Oncol. 2015;5:131. (WB)