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  • Goat F(ab')2 Anti-Mouse IgG2a, Human ads-AF647

Goat F(ab')2 Anti-Mouse IgG2a, Human ads-AF647

Cat. No.:
1082-31
Goat F(ab')2 Anti-Mouse IgG2a-Alexa Fluor® 647 antibody with minimal reactivity to human proteins for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
$286.00
Size Price (USD) Quantity
0.5 mg $286.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Isotype Control 0110-31
Specificity Reacts with the heavy chain of mouse IgG2a
Source Pepsin digest of Goat Anti-Mouse IgG2a, Human ads (SB Cat. No. 1080)
Cross Adsorption Mouse IgG1, IgG2b, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1
FLISA
Flow Cytometry 2-18

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 19,20
Immunohistochemistry-Paraffin Sections 21
Immunocytochemistry 22
RRID Number AB_2794504
Gene ID 380793 (Mouse)
Gene ID Symbol Ighg2a (Mouse)
Gene ID Aliases 1810060O09Rik; Igh-1; Igh-1a
UniProt ID P01863 (Mouse
P01864 (Mouse
P01865 (Mouse
UniProt Name GCAA_MOUSE (Mouse)
GCAB_MOUSE (Mouse)
GCAM_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Kobayashi N, Karisola P, Peña-Cruz V, Dorfman DM, Jinushi M, Umetsu SE, et al. TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells. Immunity. 2007;27:927-40. (ELISA)
  2. 2. Lovchik JA, Wilder JA, Huffnagle GB, Riblet R, Lyons CR, Lipscomb MF. Ig heavy chain complex-linked genes influence the immune response in a murine cryptococcal infection. J Immunol. 1999;163:3907-13. (FC)
  3. 3. Stabel TJ, Bolin SR, Pesch BA, Rahner TE. A simple and rapid flow cytometric method for detection of porcine cell surface markers. J Immunol Methods. 2000;245:147-52. (FC)
  4. 4. Husson H, Carideo EG, Cardoso AA, Lugli SM, Neuberg D, Munoz O, et al. MCP-1 modulates chemotaxis by follicular lymphoma cells. Br J Haematol. 2001;115:554-62. (FC)
  5. 5. Gelderman KA, Blok VT, Fleuren GJ, Gorter A. The inhibitory effect of CD46, CD55, and CD59 on complement activation after immunotherapeutic treatment of cervical carcinoma cells with monoclonal antibodies or bispecific monoclonal antibodies. Lab Invest. 2002;82:483-93. (FC)
  6. 6. Mitre E, Thompson RW, Carvalho EM, Nutman TB, Neva FA. Majority of interferon-γ-producing CD4+ cells in patients infected with human T cell lymphotrophic virus do not express tax protein. J Infect Dis. 2003;181:428-32. (FC)
  7. 7. Viglianti GA, Lau CM, Hanley TM, Miko BA, Shlomchik MJ, Marshak-Rothstein A. Activation of autoreactive B cells by CpG dsDNA. Immunity. 2003;19:837-47. (FC)
  8. 8. Boulé MW, Broughton C, Mackay F, Akira S, Marshak-Rothstein A, Rifkin IR. Toll-like receptor 9-dependent and -independent dendritic cell activation by chromatin-immunoglobulin G complexes. J Exp Med. 2004;199:1631-40. (FC)
  9. 9. Fulton BE Jr, Portella M, Radke K. Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node. J Virol. 2006;80:7873-84. (FC)
  10. 10. Meurens F, Whale J, Brownlie R, Dybvig T, Thompson DR, Gerdts V. Expression of mucosal chemokines TECK/CCL25 and MEC/CCL28 during fetal development of the ovine mucosal immune system. Immunology. 2007;120:544-55. (FC)
  11. 11. Guikema JE, Schrader CE, Brodsky MH, Linehan EK, Richards A, El Falaky N, et al. p53 represses class switch recombination to IgG2a through its antioxidant function. J Immunol. 2010;184:6177-87. (FC)
  12. 12. Stabel JR, Robbe-Austerman S. Early immune markers associated with Mycobacterium avium subsp. paratuberculosis infection in a neonatal calf model. Clin Vaccine Immunol. 2011;18:393-405. (FC)
  13. 13. Verloes A, Van de Velde H, LeMaoult J, Mateizel I, Cauffman G, Horn PA, et al. HLA-G expression in human embryonic stem cells and preimplantation embryos. J Immunol. 2011;186:2663-71. (FC)
  14. 14. Stabel JR, Waters WR, Bannantine JP, Palmer MV. Disparate host immunity to Mycobacterium avium subsp. paratuberculosis antigens in calves inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii, and M. bovis. Clin Vaccine Immunol. 2013;20:848-57. (FC)
  15. 15. Schrader CE, Linehan EK, Ucher AJ, Bertocci B, Stavnezer J. DNA polymerases β and λ do not directly affect Ig variable region somatic hypermutation although their absence reduces the frequency of mutations. DNA Repair. 2013;12:1087-93. (FC)
  16. 16. Mackenzie-Dyck S, Kovacs-Nolan J, Snider M, Babiuk LA, van Drunen Littel-van den Hurk S. Inclusion of the bovine neutrophil Beta-defensin 3 with glycoprotein D of bovine herpesvirus 1 in a DNA vaccine modulates immune responses of mice and cattle. Clin Vaccine Immunol. 2014;21:463-77. (FC)
  17. 17. Haas E, Rütgen BC, Gerner W, Richter B, Tichy A, Galler A, et al. Phenotypic characterization of canine intestinal intraepithelial lymphocytes in dogs with inflammatory bowel disease. J Vet Intern Med. 2014;28:1708-15. (FC)
  18. 18. Pasternak JA, Ng SH, Käser T, Meurens F, Wilson HL. Grouping pig-specific responses to mitogen with similar responder animals may facilitate the interpretation of results obtained in an out-bred animal model. J Vaccines Vaccin. 2014;5:5. (FC)
  19. 19. Uyemura K, Demer LL, Castle SC, Jullien D, Berliner JA, Gately MK, et al. Cross-regulatory roles of interleukin (IL)-12 and IL-10 in atherosclerosis. J Clin Invest. 1996;97:2130-8. (IHC-FS)
  20. 20. Castan J, Tenner-Racz K, Racz P, Fleischer B, Bröker BM. Accumulation of CTLA-4 expressing T lymphocytes in the germinal centres of human lymphoid tissues. Immunology. 1997;90:265-71. (IHC-FS)
  21. 21. Kanaya T, Aso H, Miyazawa K, Kido T, Minashima T, Watanabe K, et al. Staining patterns for actin and villin distinguish M cells in bovine follicle-associated epithelium. Res Vet Sci. 2007;82:141-9. (IHC-PS)
  22. 22. Peng SL, Moslehi J, Craft J. Roles of interferon-γ and interleukin-4 in murine lupus. J Clin Invest. 1997;99:1936-46. (ICC)
See All References

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