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  • Goat Anti-Mouse IgG1, Human/Bovine/Horse SP ads-AF488

Goat Anti-Mouse IgG1, Human/Bovine/Horse SP ads-AF488

Cat. No.:
1073-30
Goat Anti-Mouse IgG1-Alexa Fluor® 488 antibody with minimal reactivity to human, bovine, and horse serum proteins for use in fluorescent assays.
$391.00
Size Price (USD) Quantity
1.0 mg $391.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-AF488
Specificity Reacts with the heavy chain of mouse IgG1
Source Pooled antisera from goats hyperimmunized with mouse IgG1
Cross Adsorption Mouse IgG2a, IgG2b, IgG3, IgM, and IgA; human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG1 covalently linked to agarose
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA

RRID Number AB_2794445
Gene ID 16017 (Mouse)
Gene ID Symbol Ighg1 (Mouse)
Gene ID Aliases IgG1; Igh-4; VH7183
UniProt ID P01868 (Mouse
P01869 (Mouse
UniProt Name IGHG1_MOUSE (Mouse)
IGH1M_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Frozen newborn mouse cartilage section was stained with Mouse IgG<sub>1</sub>-UNLB isotype control (SB Cat. No. 0102-01; left) and Mouse Anti-Type II Collagen-UNLB (right) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-FITC (SB Cat. No. 1070-02) and DAPI.
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  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  3. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>-AF488 (SB Cat. No. 1071-30).
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  4. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Mouse IgG<sub>1</sub>, Human ads-AF488 (SB Cat. No. 1072-30).
    Goat F(ab')2 Anti-Mouse IgG1, Human ads-AF488
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  5. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  6. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-AF647 (SB Cat. No. 1090-31).
    Goat Anti-Mouse IgG2b, Human ads-AF647
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  7. FLISA plate was coated with Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-UNLB (SB Cat. No. 6300-01). Serially diluted Goat IgG-AF488 (SB Cat. No. 0109-30) was captured and fluorescence intensity quantified.
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  8. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>, Human ads-AF555 (SB Cat. No. 1100-32).
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  9. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-AF488 (SB Cat. No. 1070-30).
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  10. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Rat Anti-Mouse IgG<sub>1</sub>-AF488 (SB Cat. No. 1144-30).
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  11. Wdpks1Δ-1 yeast cells treated with liquid nitrogen were stained with anti-melanin followed by Goat Anti-Mouse IgM, Human ads-TRITC (SB Cat. No. 1020-03).<br/>Image from Paolo WF Jr, Dadachova E, Mandal P, Casadevall A, Szaniszlo PJ, Nosanchuk JD. Effects of disrupting the polyketide synthase gene <i>WdPKS1</i> in <i>Wangiella</i> [<i>Exophiala</i>] <i>dermatitidis</i> on melanin production and resistance to killing by antifungal compounds, enzymatic degradation, and extremes in temperature. BMC Microbiol. 2006;6:55. Figure 5(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
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  12. Frozen section of TEPC 1165 cell line pellet was stained with Goat Anti-Mouse Kappa-TXRD (SB Cat. No. 1050-07).<br/>Image from Wang H, Owens JD, Shih JH, Li M, Bonner RF, Mushinski JF. Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA. BMC Genomics. 2006;7:97. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
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  13. BALB/c mouse splenocytes were stained with Goat Anti-Mouse Lambda-PE (SB Cat. No. 1060-09) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
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  14. FLISA plate was coated with purified mouse IgE, IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgE-FITC (SB Cat. No. 1110-02).
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