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  • Goat Anti-Mouse Kappa-AP

Goat Anti-Mouse Kappa-AP

Cat. No.:
1050-04
Goat Anti-Mouse Kappa-AP antibody for use in ELISA, immunohistochemistry / immunocytochemistry, western blot, and ELISpot assays.
$231.00
Size Price (USD) Quantity
1.0 mL $231.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Specificity Reacts with mouse κ light chains
Source Pooled antisera from goats hyperimmunized with mouse κ light chains
Cross Adsorption Mouse λ light chains; may react with κ light chains from other species
Purification Method Affinity chromatography on mouse κ light chains covalently linked to agarose
Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-11
FLISA
Flow Cytometry 3,8,12,15,16,26

Other referenced applications for relevant formats include -
ELISpot 2,7,12-14
Immunohistochemistry-Frozen Sections 17
Immunohistochemistry-Paraffin Sections 18,19
Immunocytochemistry 5,20-22
Western Blot 1,4,6,8,9,15,26
Immunoprecipitation 23,24
Stimulation 25,26
Surface Plasmon Resonance 27
RRID Number AB_2794381
Gene ID 243469 (Mouse)
Gene ID Symbol Igk (Mouse)
Gene ID Aliases kappa
UniProt ID P01837 (Mouse
UniProt Name IGKC_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
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Other Conjugates and Formulations

Citations

  1. 1. Hemminki A, Niemi S, Hoffrén A, Hakalahti L, Söderlund H, Takkinen K. Specificity improvement of a recombinant anti-testosterone Fab fragment by CDRIII mutagenesis and phage display selection. Protein Eng. 1998;11:311-9. (ELISA, WB)
  2. 2. Sangster MY, Topham DJ, D'Costa S, Cardin RD, Marion TN, Myers LK, et al. Analysis of the virus-specific and nonspecific B cell response to a persistent B-lymphotropic gammaherpesvirus. J Immunol. 2000;164:1820-8. (ELISA, ELISPOT)
  3. 3. D'Agnillo P, Levine JS, Subang R, Rauch J. Prothrombin binds to the surface of apoptotic, but not viable, cells and serves as a target of lupus anticoagulant autoantibodies. J Immunol. 2003;170:3408-22. (ELISA, FC)
  4. 4. Wiens GD, Owen J. Mapping of neutralizing epitopes on Renibacterium salmoninarum p57 by use of transposon mutagenesis and synthetic peptides. Appl Environ Microbiol. 2005;71:2894-901. (ELISA, WB)
  5. 5. Chen C, Li H, Tian Q, Beardall M, Xu Y, Casanova N, et al. Selection of anti-double-stranded DNA B cells in autoimmune MRL-lpr/lpr mice. J Immunol. 2006;176:5183-90. (ELISA, ICC)
  6. 6. Subhadra B, Hurwitz I, Fieck A, Rao DV, Rao GS, Durvasula R. Development of paratransgenic Artemia as a platform for control of infectious diseases in shrimp mariculture. J Appl Microbiol. 2010;108:831-40. (ELISA, WB)
  7. 7. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Iga serine/threonines fine-tune Ig a tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA, ELISPOT)
  8. 8. Xia Y, Pawar RD, Nakouzi AS, Herlitz L, Broder A, Liu K, et al. The constant region contributes to the antigenic specificity and renal pathogenicity of murine anti-DNA antibodies. J Autoimmun. 2012;39:398-411. (ELISA, FC, WB)
  9. 9. Nakanishi K, Narimatsu S, Ichikawa S, Tobisawa Y, Kurohane K, Niwa Y, et al. Production of hybrid-IgG/IgA plantibodies with neutralizing activity against Shiga toxin 1. PLoS One. 2013;8(11):e80712. (ELISA, WB)
  10. 10. DiLillo DJ, Tan GS, Palese P, Ravetch JV. Broadly neutralizing hemagglutinin stalk-specific antibodies require FcγR interactions for protection against influenza virus in vivo. Nat Med. 2014;20:143-51. (ELISA)
  11. 11. Sang A, Niu H, Cullen J, Choi SC, Zheng YY, Wang H, et al. Activation of rheumatoid factor-specific B cells is antigen dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model. J Immunol. 2014;193:1609-21. (ELISA)
  12. 12. Seagal J, Edry E, Keren Z, Leider N, Benny O, Machluf M, et al. A fail-safe mechanism for negative selection of isotype-switched B cell precursors is regulated by the Fas/FasL pathway. J Exp Med. 2003;198:1609-19. (ELISPOT, FC)
  13. 13. Sharma S, Campbell AM, Chan J, Schattgen SA, Orlowski GM, Nayar R, et al. Suppression of systemic autoimmunity by the innate immune adaptor STING. Proc Natl Acad Sci USA. 2015;112:E710-7. (ELISPOT)
  14. 14. Ratliff M, Alter S, McAvoy K, Frasca D, Wright JA, Zinkel SS, et al. In aged mice, low surrogate light chain promotes pro-B-cell apoptotic resistance, compromises the PreBCR checkpoint, and favors generation of autoreactive, phosphorylcholine-specific B cells. Aging Cell. 2015;14:382-90. (ELISPOT)
  15. 15. Rakhmanov M. Analysis of B lymphocyte activation and differentiation by expression profiling [dissertation]. Freiburg (Germany); University of Freiburg: 2005. (FC, WB)
  16. 16. Campbell KS, Yusa S, Kikuchi-Maki A, Catina TL. NKp44 triggers NK cell activation through DAP12 association that is not influenced by a putative cytoplasmic inhibitory sequence. J Immunol. 2004;172:899-906. (FC)
  17. 17. Harris DP, Vogel P, Wims M, Moberg K, Humphries J, Jhaver KG, et al. Requirement for class II phosphoinositide 3-kinase C2α in maintenance of glomerular structure and function. Mol Cell Biol. 2011;31:63-80. (IHC-FS)
  18. 18. Verdonk RC, Haagsma EB, Jonker MR, Bok LI, Zandvoort JH, Kleibeuker JH, et al. Effects of different immunosuppressive regimens on regulatory T-cells in noninflamed colon of liver transplant recipients. Inflamm Bowel Dis. 2007;13:703-9. (IHC-PS)
  19. 19. Weinstein JS, Nacionales DC, Lee PY, Kelly-Scumpia KM, Yan X, Scumpia PO, et al. Colocalization of antigen-specific B and T cells within ectopic lymphoid tissue following immunization with exogenous antigen. J Immunmol. 2008;181:3259-67. (IHC-PS)
  20. 20. Gollahon KA, Hagman J, Brinster RL, Storb U. Ig λ-producing B cells do not show feedback inhibition of gene rearrangement. J Immunol. 1988;141:2771-80. (ICC)
  21. 21. Duchez S, Amin R, Cogné N, Delpy L, Sirac C, Pascal V, et al. Premature replacement of μ with α immunoglobulin chains impairs lymphopoiesis and mucosal homing but promotes plasma cell maturation. Proc Natl Acad Sci USA. 2010;107:3064-9. (ICC)
  22. 22. Wang N, Yuan A, Ma J, Deng Z, Xue L. Sexing murine embryos with an indirect immunofluorescence assay using phage antibody B9-Fab against SDM antigen. J Vet Med Sci. 2015;77:711-4. (ICC)
  23. 23. Skowronek MH, Hendershot LM, Haas IG. The variable domain of nonassembled Ig light chains determines both their half-life and binding to the chaperone BiP. Proc Natl Acad Sci USA. 1998;95:1574-8. (IP)
  24. 24. Otero JH, Lizák B, Feige MJ, Hendershot LM. Dissection of structural and functional requirements that underlie the interaction of ERdj3 protein with substrates in the endoplasmic reticulum. J Biol Chem. 2014;289:27504-12. (IP)
  25. 25. Ku P, You M, Bose HR Jr. Role and regulation of Rel/NF-κB activity in anti-immunoglobulin-induced apoptosis in WEHI-231 B lymphoma cells. Cell Signal. 2000;12:245-53. (Stim)
  26. 26. Übelhart R, Hug E, Bach MP, Wossning T, Dühren-von Minden M, Horn AH, et al. Responsiveness of B cells is regulated by the hinge region of IgD. Nat Immunol. 2015;16:534-43. (FC, WB, Stim)
  27. 27. Swearingen CA, Carpenter JW, Siegel R, Brittain IJ, Dotzlaf J, Durham TB, et al. Development of a novel clinical biomarker assay to detect and quantify aggrecanase-generated aggrecan fragments in human synovial fluid, serum and urine. Osteoarthritis Cartilage. 2010;18:1150-8. (Surface Plasmon Resonance)
See All References

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