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  • Goat F(ab')2 Anti-Mouse Ig, Human ads-AF647

Goat F(ab')2 Anti-Mouse Ig, Human ads-AF647

Cat. No.:
1012-31
Goat F(ab')2 Anti-Mouse Ig-Alexa Fluor® 647 antibody with minimal reactivity to human proteins for use in flow cytometry, immunocytochemistry, and western blot assays.
$185.00
Size Price (USD) Quantity
0.5 mg $185.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat F(ab')2 IgG
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, and IgA
Source Pepsin digest of Goat Anti-Mouse Ig, Human ads (SB Cat. No. 1010)
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1-6
FLISA
Flow Cytometry 8-21

Other referenced applications for relevant formats include -
ELISpot 5,7
Immunocytochemistry 22,23
Western Blot 24
Stimulation 25-27
RRID Number AB_2794177

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Gerth AJ, Lin L, Peng SL. T-bet regulates T-independent IgG2a class switching. Int Immunol. 2003;15:937-44. (ELISA)
  2. 2. Oble DA, Collett E, Hsieh M, Ambjørn M, Law J, Dutz J, et al. A novel T cell receptor transgenic animal model of seborrheic dermatitis-like skin disease. J Invest Dermatol. 2005;124:151-9. (ELISA)
  3. 3. Kang T, Oh G, Scandella E, Bolinger B, Ludewig B, Kovalenko A, et al. Mutation of a self-processing site in caspase-8 compromises its apoptotic but not its nonapoptotic functions in bacterial artificial chromosome-transgenic mice. J Immunol. 2008;181:2522-32. (ELISA)
  4. 4. Seo KY, Han SJ, Cha H, Seo S, Song J, Chung S, et al. Eye mucosa: an efficient vaccine delivery route for inducing protective immunity. J Immunol. 2010;185:3610-9. (ELISA)
  5. 5. Chang J, Cha H, Chang S, Ko H, Seo S, Kweon M. IFN-γ secreted by CD103+ dendritic cells leads to IgG generation in the mesenteric lymph node in the absence of vitamin A. J Immunol. 2011;186:6999-7005. (ELISA, ELISPOT)
  6. 6. Sun Y, Peng I, Senger K, Hamidzadeh K, Reichelt M, Baca M, et al. Critical role of activation induced cytidine deaminase in experimental autoimmune encephalomyelitis. Autoimmunity. 2013;46:157-67. (ELISA)
  7. 7. Cha H, Ko H, Kim E, Chang S, Seo S, Cuburu N, et al. Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control. J Immunol. 2011;187:3044-52. (ELISPOT)
  8. 8. Coccia MA, Brams P. High titer, prostate specific antigen-specific human IgG production by hu-PBL-SCID mice immunized with antigen-mouse IgG2a complex-pulsed autologous dendritic cells. J Immunol. 1998;161:5772-80. (FC)
  9. 9. Lund FE, Muller-Steffner HM, Yu N, Stout CD, Schuber F, Howard MC. CD38 signaling in B lymphocytes is controlled by its ectodomain but occurs independently of enzymatically generated ADP-ribose or cyclic ADP-ribose. J Immunol. 1999;162:2693-702. (FC)
  10. 10. Reeb-Whitaker CK, Paigen B, Beamer WG, Bronson RT, Churchill GA, Schweitzer IB, et al. The impact of reduced frequency of cage changes on the health of mice housed in ventilated cages. Lab Anim. 2001;35:58-73. (FC)
  11. 11. Musso T, Deaglio S, Franco L, Calosso L, Badolato R, Garbarino G, et al. CD38 expression and functional activities are up-regulated by IFN-γ on human monocytes and monocytic cell lines. J Leukoc Biol. 2001;69:605-12. (FC)
  12. 12. Gliddon DR, Howard CJ. CD26 is expressed on a restricted subpopulation of dendritic cells in vivo. Eur J Immunol. 2002;32:1472-81. (FC)
  13. 13. Bian H, Fournier P, Moormann R, Peeters B, Schirrmacher V. Selective gene transfer in vitro to tumor cells via recombinant Newcastle disease virus. Cancer Gene Ther. 2005;12:295-303. (FC)
  14. 14. Richman SA, Healan SJ, Weber KS, Donermeyer DL, Dossett ML, Greenberg PD, et al. Development of a novel strategy for engineering high-affinity proteins by yeast display. Protein Eng Des Sel. 2006;19:255-64. (FC)
  15. 15. Janke M, Peeters B, de Leeuw O, Moorman R, Arnold A, Fournier P, et al. Recombinant Newcastle disease virus (NDV) with inserted gene coding for GM-CSF as a new vector for cancer immunogene therapy. Gene Ther. 2007;14:1639-49. (FC)
  16. 16. Apostolidis L, Schirrmacher V, Fournier P. Host mediated anti-tumor effect of oncolytic Newcastle disease virus after locoregional application. Int J Oncol. 2007;31:1009-19. (FC)
  17. 17. Sopp P, Werling D, Baldwin C. Cross-reactivity of mAbs to human CD antigens with cells from cattle. Vet Immunol Immunopathol. 2007;119:106-14. (FC)
  18. 18. Yu KO, Im JS, Illarianov PA, Ndonye RM, Howell AR, Besra GS, et al. Production and characterization of monoclonal antibodies against complexes of the NKT cell ligand α-galactosylceramide bound to mouse CD1d. J Immunol Methods. 2007;323:11-23. (FC)
  19. 19. Janke M, Peeters B, Zhao H, de Leeuw O, Moorman R, Arnold A, et al. Activation of human T cells by a tumor vaccine infected with recombinant Newcastle disease virus producing IL-2. Int J Oncol. 2008;33:823-32. (FC)
  20. 20. Pfirschke C, Schirrmacher V. Cross-infection of tumor cells by contact with T lymphocytes loaded with Newcastle disease virus. Int J Oncol. 2009;34:951-62. (FC)
  21. 21. Fournier P, Aigner M, Schirrmacher V. Transcriptome analysis and cytokine profiling of naive T cells stimulated by a tumor vaccine via CD3 and CD25. Int J Oncol. 2010;37:1439-52. (FC)
  22. 22. Saito E, Fujimoto M, Hasegawa M, Komura K, Hamaguchi Y, Kaburagi Y, et al. CD19-dependent B lymphocyte signaling thresholds influence skin fibrosis and autoimmunity in the tight-skin mouse. J Clin Invest. 2002;109:1453-62. (ICC)
  23. 23. Pilling D, Buckley CD, Salmon M, Gomer RH. Inhibition of fibrocyte differentiation by serum amyloid P. J Immunol. 2003;171:5537-46. (ICC)
  24. 24. Gan M, Moussaud S, Jiang P, McLean PJ. Extracellular ATP induces intracellular alpha-synuclein accumulation via P2X1 receptor-mediated lysosomal dysfunction. Neurobiol Aging. 2015;36:1209-20. (WB)
  25. 25. Do Y, Rafi-Janajreh AQ, Mckallip RJ, Nagarkatti PS, Nagarkatti M. Combined deficiency in CD44 and Fas leads to exacerbation of lymphoproliferative and autoimmune disease. Int Immunol. 2003;15:1327-40. (Stim)
  26. 26. Liubchenko GA, Appleberry HC, Holers VM, Banda NK, Willis VC, Lyubchenko T. Potentially autoreactive naturally occurring transitional T3 B lymphocytes exhibit a unique signaling profile. J Autoimmun. 2012;38:293-303. (Stim)
See All References

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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  5. FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-TXRD (SB Cat. No. 3052-07).
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