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Rat Anti-Human GM-CSF-LE/AF (BVD2-23B6)
Cat. No.:
10111-14
Low Endotoxin/Azide Free Anti-Human GM-CSF antibody for use in ELISA, ELISpot, immunoprecipitation, multiplex, and neutralization assays.
$308.00
| Clone | BVD2-23B6 |
|---|---|
| Isotype | Rat IgG2aκ |
| Isotype Control | Rat IgG2a-LE/AF (KLH/G2a-1-1) |
| Specificity | Human/Rhesus/Cynomolgus GM-CSF |
| Alternative Names | Granulocyte/macrophage colony-stimulating factor, CSF-α, pluripoietin-α, eosinophil colony stimulating factor, Eo-CSF, burst promoting activity, BPA |
| Immunogen | E. coli-expressed human GM-CSF |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications |
ELISA-Capture – Quality tested 1-7 ELISpot-Capture – Reported in literature 4,6,8 Immunoprecipitation – Reported in literature 3 Neutralization – Reported in literature 1,3,9,10 Multiplex-Capture – Reported in literature 11 Note – May be paired with the biotinylated clone BVD2-21C11 (SB Cat. No. 10112-08) in a sandwich ELISA |
| RRID Number | AB_2794136 |
| Gene ID |
1437 (Human) 574371 (Rhesus) 102118747 (Cynomolgus) |
| Gene ID Symbol |
CSF2 (Human) CSF2 (Rhesus) CSF2 (Cynomolgus) |
| Gene ID Aliases | CSF; GMCSF; GM-CSF |
| UniProt ID |
P04141 (Human |
| UniProt Name |
CSF2_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Abrams JS, Silver JE, Van Dyke RE, Gleich GJ. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GM-CSF. In: Gleich GJ, Kay AB, editors. Eosinophils in allergy and inflammation. New York: Marcel Dekker; 1994. p. 133-57. (Immunogen, ELISA-Capture, Neut)
- 2. Bacchetta R, de Waal Malefijt R, Yssel H, Abrams J, de Vries JE, Spits H, et al. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-γ and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990;144:902-8. (ELISA-Capture)
- 3. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, IP, Neut)
- 4. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Capture, ELISPOT-Capture)
- 5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
- 6. Mäkitalo B, Andersson M, Areström I, Karlén K, Villinger F, Ansari A, et al. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods. 2002;270:85-97. (ELISA-Capture, ELISPOT-Capture, Rhesus & Cynomolgus Reactivity)
- 7. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
- 8. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
- 9. Denburg JA, Silver JE, Abrams JS. Interleukin-5 is a human basophilopoietin: induction of histamine content and basophilic differentiation of HL-60 cells and of peripheral blood basophil-eosinophil progenitors. Blood. 1991;77:1462-8. (Neut)
- 10. Kelly EA, Koziol-White CJ, Clay KJ, Liu LY, Bates ME, Bertics PJ, et al. Potential contribution of IL-7 to allergen-induced eosinophilic airway inflammation in asthma. J Immunol. 2009;182:1404-10. (Neut)
- 11. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Capture, Cynomolgus Reactivity)
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