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Mouse Anti-Bcl-2-UNLB (10C4)
Cat. No.:
10065-01
Purified Anti-Bcl-2 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blot, and immunoprecipitation assays.
$198.00
| Clone | 10C4 |
|---|---|
| Isotype | Mouse IgG1κ |
| Isotype Control | Mouse IgG1-UNLB (15H6) |
| Specificity | Mouse/Rat Bcl-2 |
| Alternative Names | B cell leukemia 2 |
| Description | Bcl-2 is a 26 kDa member of the family of proteins involved in regulation of programmed cell death, or apoptosis. It is expressed in a variety of both normal and neoplastic tissues and appears to be membrane-bound since it is detectable in crude membrane and nuclear fractions, but not in the soluble fraction of cell lysates. Overexpression of Bcl-2 can prevent apoptosis, while formation of heterodimers with Bax, another Bcl-2-related protein, inhibits Bcl-2’s ability to promote cell survival. |
| Immunogen | Murine Bcl-2 peptide (a.a. 61-76) |
| Conjugate | UNLB (Unconjugated) |
| Buffer Formulation | Borate buffered saline, pH 8.2 |
| Clonality | Monoclonal |
| Concentration | 0.1 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications |
Western Blot – Quality tested 1-4 Immunoprecipitation – Reported in literature 3,4 Flow Cytometry – Reported in literature 5,6 Immunohistochemistry-Frozen Sections – Reported in literature 7 Immunohistochemistry-Paraffin Sections – Reported in literature 8 Immunocytochemistry – Reported in literature 3 |
| RRID Number | AB_2794109 |
| Gene ID |
12043 (Mouse) 24224 (Rat) |
| Gene ID Symbol |
Bcl2 (Mouse) Bcl2 (Rat) |
| Gene ID Aliases | AW986256; Bcl-2; C430015F12Rik; D630044D05Rik; D830018M01Rik |
| UniProt ID |
P10417 (Mouse P49950 (Rat |
| UniProt Name |
BCL2_MOUSE (Mouse) BCL2_RAT (Rat) |
Documentation
Certificate of Analysis Lookup
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- 1. Hsu Y, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997;272:13829-34. (Immunogen, WB)
- 2. Hsu Y, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-XL during apoptosis. Proc Natl Acad Sci USA. 1997;94:3668-72. (WB)
- 3. Conus S, Kaufmann T, Fellay I, Otter I, Rossé T, Borner C. Bcl-2 is a monomeric protein: prevention of homodimerization by structural constraints. EMBO J. 2000;19:1534-44. (WB, IP, ICC)
- 4. Gaudette BT, Iwakoshi NN, Boise LH. Bcl-xL protein protects from C/EBP homologous protein (CHOP)-dependent apoptosis during plasma cell differentiation. J Biol Chem. 2014;289:23629-40. (WB, IP)
- 5. McFarland HI, Puig M, Grajkowska LT, Tsuji K, Lee JP, Mason KP, et al. Regulatory T cells in γ irradiation-induced immune suppression. PLoS One. 2012;7(6):e39092. (FC)
- 6. Wang X, Szymczak-Workman AL, Gravano DM, Workman CJ, Green DR, Vignali DA. Preferential control of induced regulatory T cell homeostasis via a Bim/Bcl-2 axis. Cell Death Dis. 2012;3:e270. (FC)
- 7. Yamanaka T, Helgeland L, Farstad IN, Fukushima H, Midtvedt T, Brandtzaeg P. Microbial colonization drives lymphocyte accumulation and differentiation in the follicle-associated epithelium of Peyer's patches. J Immunol. 2003;170:816-22. (IHC-FS)
- 8. Saatli B, Kizildag S, Posaci C, Dogan E, Koyuncuoglu M, Ulukus EC, et al. Long-term effects of GnRH agonist, GnRH antagonist, and estrogen plus progesterone treatment on apoptosis related genes in rat ovary. Fertil Steril. 2009;91:2006-11. (IHC-PS)
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