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SBA Clonotyping System-HRP

Cat. No.:
5300-05
HRP Mouse Clonotyping System for use in the isotyping of mouse monoclonal antibodies and for quantitative studies of mouse immunoglobulins in samples such as serum, supernatant, and ascites.
$457.00
Size Price (USD) Quantity
1 kit $457.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Description The SBA Clonotyping System-HRP kit is designed for the isotyping of mouse monoclonal antibodies. It contains 2.5 mg of capture antibody and 1.0 mL of HRP conjugated anti-mouse Ig, mouse IgA, mouse IgG1, mouse IgG2a, mouse IgG2b, mouse IgG3, mouse IgM, mouse κ, mouse λ, and ABTS substrate. The kit may also be utilized for quantitative studies of mouse immunoglobulins in samples such as serum, supernatant, and ascites when used in conjunction with the Mouse Immunoglobulin Panel (SB Cat. No. 5300-01).
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation UNLB antibody - Borate buffered saline, pH 8.2 HRP conjugates - 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Recommended Storage 2-8°C
Kit Components Goat Anti-Mouse Ig, Human ads-UNLB - 2.5 mg in 1.0 mL
Goat Anti-Mouse Ig, Human ads-HRP - 1.0 mL
Goat Anti-Mouse IgA-HRP - 1.0 mL
Goat Anti-Mouse IgG1, Human ads-HRP - 1.0 mL
Goat Anti-Mouse IgG2a, Human ads-HRP - 1.0 mL
Goat Anti-Mouse IgG2b, Human ads-HRP - 1.0 mL
Goat Anti-Mouse IgG3, Human ads-HRP - 1.0 mL
Goat Anti-Mouse IgM, Human ads-HRP - 1.0 mL
Goat Anti-Mouse Kappa-HRP - 1.0 mL
Goat Anti-Mouse Lambda-HRP - 1.0 mL
ABTS Substrate Powder - 100 mg
Applications ELISA – Quality tested 1-42

RRID Number AB_2796080

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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Other Conjugates and Formulations

Citations

  1. 1. Tseng T, Chung C. A monoclonal antibody recognizing the specific epitope of aflatoxin analogs. Bot Bull Acad Sin. 1990;31:279-84. (ELISA - Isotyping)
  2. 2. Yu L, Lee KK, Ens K, Doig PC, Carpenter MR, Staddon W, et al. Partial characterization of a Candida albicans fimbrial adhesin. Infect Immun. 1994;62:2834-42. (ELISA - Isoyping)
  3. 3. Esteban MR, Giovinazzo G, de la Hera A, Goday C. PUMA1: a novel protein that associates with the centrosomes, spindle and centromeres in the nematode Parascaris. J Cell Sci. 1998;111:723-35. (ELISA - Isotyping)
  4. 4. Gil OD, Zhang L, Chen S, Ren YQ, Pimenta A, Zanazzi G, et al. Complementary expression and heterophilic interactions between IgLON family members neurotrimin and LAMP. J Neurobiol. 2002;51:190-204. (ELISA - Isotyping)
  5. 5. Sánchez MD, Pierson TC, McAllister D, Hanna SL, Puffer BA, Valentine LE, et al. Characterization of neutralizing antibodies to West Nile virus. Virology. 2005;336:70-82. (ELISA - Isotyping)
  6. 6. Mazzarotto GA, Raboni SM, Stella V, Carstensen S, de Noronha L, Levis S, et al. Production and characterization of monoclonal antibodies against the recombinant nucleoprotein of Araucaria hantavirus. J Virol Methods. 2009;162:96-100. (ELISA - Isotyping)
  7. 7. Wang C, deVries S, Camboni M, Glass M, Martin PT. Immunization with the SDPM1 peptide lowers amyloid plaque burden and improves cognitive function in the APPswePSEN1(A246E) transgenic mouse model of Alzheimer's disease. Neurobiol Dis. 2010;39:409-22. (ELISA - Isotyping & Serum)
  8. 8. Vassiliadis E, Veidal SS, Simonsen H, Larsen DV, Vainer B, Chen X, et al. Immunological detection of the type V collagen propeptide fragment, PVCP-1230, in connective tissue remodeling associated with liver fibrosis. Biomarkers. 2011;16:426-33. (ELISA - Isotyping)
  9. 9. Liu Z, Song C, Li Y, Liu F, Zhang K, Sun Y, et al. Development of highly sensitive chemiluminescence enzyme immunoassay based on the anti-recombinant HC subunit of botulinum neurotoxin type A monoclonal antibodies. Anal Chim Acta. 2012;735:23-30. (ELISA - Isotyping)
  10. 10. Han Z, Zhao F, Shao Y, Liu X, Kong X, Song Y, et al. Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein. Virus Res. 2013;171:54-64. (ELISA - Isotyping)
  11. 11. Wongwairot S, Kueakhai P, Changklungmoa N, Jaikua W, Sansri V, Meemon K, et al. Monoclonal antibody against recombinant Fasciola gigantica cathepsin L1H could detect juvenile and adult cathepsin Ls of Fasciola gigantica. Parasitol Res. 2015;114:133-40. (ELISA - Isotyping)
  12. 12. Ren J, Zhu Y, Zhou Y, Lv C, Yan H, Ma L, et al. Identification of three antigen epitopes on the nucleocapsid protein of the genotype C of bovine parainfluenza virus type 3. Vet Microbiol. 2015;178:61-9. (ELISA - Isotyping)
  13. 13. Guo C, Tang Y, Qi Z, Liu Y, Zhao X, Huo X, et al. Development and characterization of a panel of cross-reactive monoclonal antibodies generated using H1N1 influenza virus. Immunobiology. 2015;220:941-6. (ELISA - Isotyping)
  14. 14. Li C, Liang W, Liu W, Yang D, Wang H, Ma W, et al. Identification of a conserved linear epitope using a monoclonal antibody against non-structural protein 3B of foot-and-mouth disease virus. Arch Virol. 2016;161:365-75. (ELISA - Isotyping)
  15. 15. Gu J, Wang L, Jin Y, Lin C, Wang H, Zhou N, et al. Characterization of specific antigenic epitopes and the nuclear export signal of the Porcine circovirus 2 ORF3 protein. Vet Microbiol. 2016;184:40-50. (ELISA - Isotyping)
  16. 16. Hansen NU, Willumsen N, Sand JM, Larsen L, Karsdal MA, Lemming DJ. Type VIII collagen is elevated in diseases associated with angiogenesis and vascular remodeling. Clin Biochem. 2016 May 24. doi: 10.1016/j.clinbiochem.2016.05.023. [Epub ahead of print]. (ELISA - Isotyping)
  17. 17. Cascalho M, Wong J, Wabl M. VH gene replacement in hyperselected B cells of the quasimonoclonal mouse. J Immunol. 1997;159:5795-801. (ELISA - Serum)
  18. 18. Kikuchi T, Hackett NR, Crystal RG. Cross-strain protection against clinical and laboratory strains of Pseudomonas aeruginosa mediated by dendritic cells genetically modified to express CD40 ligand and pulsed with specific strains of Pseudomonas aeruginosa. Hum Gene Ther. 2001;12:1251-63. (ELISA - Serum)
  19. 19. Wu X, Jiang N, Deppong C, Singh J, Dolecki G, Mao D, et al. A role for the Cr2 gene in modifying autoantibody production in systemic lupus erythematosus. J Immunol. 2002;169:1587-92. (ELISA - Serum)
  20. 20. Ruzek MC, Hawes M, Pratt B, McPherson J, Ledbetter S, Richards SM, et al. Minimal effects on immune parameters following chronic anti-TGF-β monoclonal antibody administration to normal mice. Immunopharmacol Immunotoxicol. 2003;25:235-57. (ELISA - Serum)
  21. 21. Olson TS, Bamias G, Naganuma M, Rivera-Nieves J, Burcin TL, Ross W, et al. Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease. J Clin Invest. 2004;114:389-98. (ELISA - Serum & Supernatant)
  22. 22. Coughlin JJ, Stang SL, Dower NA, Stone JC. RasGRP1 and RasGRP3 regulate B cell proliferation by facilitating B cell receptor-Ras signaling. J Immunol. 2005;175:7179-84. (ELISA - Serum)
  23. 23. Perchonock CE, Fernando MC, Quinn WJ 3rd, Nguyen CT, Sun J, Shapiro MJ, et al. Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006;26:6005-15. (ELISA - Serum)
  24. 24. Travis MA, Reizis B, Melton AC, Masteller E, Tang Q, Proctor JM, et al. Loss of integrin αvβ8 on dendritic cells causes autoimmunity and colitis in mice. Nature. 2007;449:361-5. (ELISA - Serum)
  25. 25. Bell JJ, Divekar RD, Ellis JS, Cascio JA, Haymakker CL, Jain R, et al. In trans T cell tolerance diminishes autoantibody responses and exacerbates experimental allergic encephalomyelitis. J Immunol. 2008;180:1508-16. (ELISA - Serum)
  26. 26. Bolduc A, Long E, Stapler D, Cascalho M, Tsubata T, Koni PA, et al. Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J Immunol. 2010;185:220-30. (ELISA - Serum)
  27. 27. Bohgaki T, Bohgaki M, Cardoso R, Panier S, Zeegers D, Li L, et al. Genomic instability, defective spermatogenesis, immunodeficiency, and cancer in a mouse model of the RIDDLE syndrome. PLoS Genet. 2011;7(4):e1001381. (ELISA - Serum & Supernatant)
  28. 28. Huang C, Hatzi K, Melnick A. Lineage-specific functions of Bcl-6 in immunity and inflammation are mediated by distinct biochemical mechanisms. Nat Immunol. 2013;14:380-8. (ELISA - Serum)
  29. 29. Huang Q, Perlman H, Birkett R, Doyle R, Fang D, Haines GK, et al. CD11c-mediated deletion of Flip promotes autoreactivity and inflammatory arthritis. Nat Commun. 2015;6:7086. (ELISA - Serum)
  30. 30. Ying Z, Mei M, Zhang P, Liu C, He H, Gao F, et al. Histone arginine methylation by PRMT7 controls germinal center formation via regulating Bcl6 transcription. J Immunol. 2015;195:1538-47. (ELISA - Serum)
  31. 31. Benhamron S, Pattanayak SP, Berger M, Tirosh B. mTOR activation promotes plasma cell differentiation and bypasses XBP-1 for immunoglobulin secretion. Mol Cell Biol. 2015;35:153-66. (ELISA - Serum & Supernatant)
  32. 32. Zanotti KJ, Maul RW, Castiblanco DP, Yang W, Choi YJ, Fox JT, et al. ATAD5 deficiency decreases B cell division and Igh recombination. J Immunol. 2015;194:35-42. (ELISA - Serum)
  33. 33. Chen J, Li Z, Petersen E, Liu W, Zhu X. Co-administration of interleukins 7 and 15 with DNA vaccine improves protective immunity against Toxoplasma gondii. Exp Parasitol. 2016;162:18-23. (ELISA - Serum)
  34. 34. Zhao Y, Li Z, Chen J, Sun X, Liu S, Zhu X, ey al. Protective efficacy of pVAX-RON5p against acute and chronic infections of Toxoplasma gondii in BALB/c mice. Exp Parasitol. 2016;163:24-30. (ELISA - Serum)
  35. 35. Corcos D, Iglesias A, Dunda O, Bucchini D, Jami J. Allelic exclusion in transgenic mice expressing a heavy chain disease-like human μ protein. Eur J Immunol. 1991;21:2711-6. (ELISA - Supernatant)
  36. 36. Ishida K, Yoshimura K. Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis. Parasite Immunol. 1992;14:633-44. (ELISA - Supernatant)
  37. 37. Yamada T, Mitani T, Yorita K, Uchida D, Matsushima A, Iwamasa K, et al. Abnormal immune function of hemopoietic cells from alymphoplasia (aly) mice, a natural strain with mutant NF-κB-inducing kinase. J Immunol. 2000;165:804-12. (ELISA - Supernatant)
  38. 38. Wittman VP, Woodburn D, Nguyen T, Neethling FA, Wright S, Weidanz JA. Antibody targeting to a class I MHC-peptide epitope promotes tumor cell death. J Immunol. 2006;177:4187-95. (ELISA - Supernatant)
  39. 39. Verma B, Hawkins OE, Neethling FA, Caseltine SL, Largo SR, Hildebrand WH, et al. Direct discovery and validation of a peptide/MHC epitope expressed in primary human breast cancer cells using a TCRm monoclonal antibody with profound antitumor properties. Cancer Immunol Immunother. 2010;59:563-73. (ELISA - Supernatant)
  40. 40. Zhang L, Zhang H, Fan Z, Zhou X, Yu L, Sun H, et al. Identification of a conserved linear B-cell epitope of Streptococcus dysgalactiae GapC protein by screening phage-displayed random peptide library. PLoS One. 2015;10(6):e0131221. (ELISA - Supernatant & Ascites)
  41. 41. Zhang J, Li H, Huo R, Zhai T, Li H, Sun Y, et al. Paeoniflorin selectively inhibits LPS-provoked B-cell function. J Pharmacol Sci. 2015;128:8-16. (ELISA - Supernatant)
  42. 42. Zhang L, Zhou X, Fan Z, Tang W, Chen L, Dai J, et al. Identification of a conserved B-cell epitope on the GapC protein of Streptococcus dysgalactiae. Microb Pathog. 2015;83-84:23-8. (ELISA - Supernatant & Ascites)
See All References

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