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Rat IgG2a-AF647 (KLH/G2a-1-1)
Cat. No.:
0117-31
Rat IgG2a-Alexa Fluor® 647 for use as a control in flow cytometry and immunohistochemistry assays.
$168.00
| Clone | KLH/G2a-1-1 |
|---|---|
| Isotype | Rat IgG2aκ |
| Specificity | KLH |
| Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications |
Flow Cytometry – Quality tested 1-7 ELISA – Quality tested FLISA – Quality tested Immunohistochemistry-Frozen Sections – Reported in literature 8 Immunohistochemistry-Paraffin Sections 14 Immunoprecipitation – Reported in literature 9 Blocking – Reported in literature 10 In vitro Control – Reported in literature 11 In vivo Control – Reported in literature 12,13 |
| RRID Number | AB_2794011 |
Documentation
Certificate of Analysis Lookup
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- 1. Welner R, Swett DJ, Pelsue SC. Age-related loss of bone marrow pre-B- and immature B-lymphocytes in the autoimmune-prone flaky skin mutant mice. Autoimmunity. 2005;38:399-408. (FC)
- 2. Gillan V, Devaney E. Regulatory T cells modulate Th2 responses induced by Brugia pahangi third-stage larvae. Infect Immun. 2005;73:4034-42. (FC)
- 3. Friesen C, Glatting G, Koop B, Schwarz K, Morgenstern A, Apostolidis C, et al. Breaking chemoresistance and radioresistance with [213Bi]anti-CD45 antibodies in leukemia cells. Cancer Res. 2007;67:1950-8. (FC)
- 4. Gillan V, Lawrence RA, Devaney E. B cells play a regulatory role in mice infected with the L3 of Brugia pahangi. Int Immunol. 2005;17:373-82. (FC)
- 5. Chu VT, Fröhlich A, Steinhauser G, Scheel T, Roch T, Fillatreau S, et al. Eosinophils are required for the maintenance of plasma cells in the bone marrow. Nat Immunol. 2011;12:151-60. (FC)
- 6. Benhamron S, Reiner I, Zcharia E, Atallah M, Grau A, Vlodavsky I, et al. Dissociation between mature phenotype and impaired transmigration in dendritic cells from heparanase-deficient mice. PLoS One. 2012;7(5):e35602. (FC)
- 7. Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. (FC)
- 8. Løvås T, Bruusgaard JC, Øynebråten I, Gundersen K, Bogen B. DNA vaccines: MHC II-targeted vaccine protein produced by transfected muscle fibres induces a local inflammatory cell infiltrate in mice. PLoS One. 2014;9(10):e108069. (IHC-FS)
- 9. Rosenbaum M, Andreani V, Kapoor T, Herp S, Flach H, Duchniewicz M, et al. MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress. Genes Dev. 2014;28:1165-78. (IP)
- 10. Talkington J, Nickell SP. Role of Fc gamma receptors in triggering host cell activation and cytokine release by Borrelia burgdorferi. Infect Immun. 2001;69:413-9. (Block)
- 11. Mayo L, Jacob-Hirsch J, Amariglio N, Rechavi G, Moutin M, Lund FE, et al. Dual role of CD38 in microglial activation and activation-induced cell death. J Immunol. 2008;181:92-103. (In vitro control)
- 12. Kaplan RN, Riba RD, Zacharoulis S, Bramley AH, Vincent L, Costa C, et al. VEGFR1-positive haematopoietic bone marrow progenitors initiate the pre-metastatic niche. Nature. 2005;438:820-7. (In vivo control)
- 13. Etich J, Bergmeier V, Frie C, Kreft S, Bengestrate L, Eming S, et al. PECAM1+/Sca1+/CD38+ vascular cells transform into myofibroblast-like cells in skin wound repair. PloS One. 2013;8(1):e53262. (In vivo control)
- 14. SouthernBiotech unpublished data (IHC-PS)
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