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  • Rat Anti-Rabbit Ig-BIOT (SB87a)

Rat Anti-Rabbit Ig-BIOT (SB87a)

Cat. No.:
4065-08
Biotin Anti-Rabbit Ig antibody for use in ELISA and flow cytometry assays.
$192.00
Size Price (USD) Quantity
0.5 mg $192.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone SB87a
Isotype Rat (Sprague Dawley) IgG2aκ
Isotype Control Rat IgG2a-BIOT (KLH/G2a-1-1)
Specificity Rabbit IgG, IgM, and IgA
Minimal reactivity to human, mouse, rat, hamster, goat, sheep, porcine, canine, and rhesus IgG and guinea pig serum
Immunogen Rabbit IgG and IgM
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested 1
FLISA – Quality tested
Flow Cytometry – Quality tested 2

RRID Number AB_2795988

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Sang Y, Zhou T, Li H, Ran Y, Jiang D, Zheng F, et al. A new long-acting GLP-1 derivative KTP ameliorates hyperglycemia and dyslipidemia and improves pancreas and fatty liver in db/db mice. Chin Sci Bull. 2013;58:2447-53. (ELISA)
  2. 2. Fernando J, Faber TW, Pullen NA, Falanga YT, Kolawole EM, Oskeritzian CA, et al. Genotype-dependent effects of TGF-β1 on mast cell function: targeting the Stat5 pathway. J Immunol. 2013;191:4505-13. (FC)

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  1. Rabbit peripheral blood lymphocytes were stained with Rat IgG<sub>2a</sub>-BIOT (SB Cat. No. 0117-08; gray) and Rat Anti-Rabbit Ig-BIOT (SB Cat. No. 4065-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
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  2. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  3. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-BIOT (SB Cat. No. 4054-08) followd by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-BIOT
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  4. ELISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  5. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
    Rabbit IgG-UNLB
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  6. ELISA plate was coated with purified rabbit IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit Ig, Human ads-BIOT (SB Cat. No. 4010-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit Ig, Human ads-BIOT
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  7. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  8. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG(H+L), Mouse/Human/Goat/Sheep SP ads-BIOT (SB Cat. No. 4091-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  9. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
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  10. ELISA plate was coated with purified rabbit IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  11. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgG-BIOT
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  12. Chicken sperm was stained with anti-CaMKIV followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Streptavidin-CY2 (SB Cat. No. 7100-21), and DAPI.<br/>Image from Nguyen TM, Combarnous Y, Praud C, Duittoz A, Blesbois E. Ca<sup>2+</sup>/calmodulin-dependent protein kinase kinases (CaMKKs) effects on AMP-activated protein kinase (AMPK) regulation of chicken sperm functions. PLoS One. 2016;11(1):e0147559. Figure 4(f)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  13. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
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  14. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  15. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG-BIOT (SB Cat. No. 4090-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Rabbit IgG-BIOT (2A9)
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  16. ELISA plate was coated with purified rabbit IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fab-BIOT (SB Cat. No. 4040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  17. ELISA plate was coated with serially diluted Rabbit F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0112-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG F(ab')<sub>2</sub>-BIOT and Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  18. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4052-08) followed Streptavidin-HRP (SB Cat. No. 7100-05).
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