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Rat Anti-Mouse Ly-6A/E-AF647 (D7)
Cat. No.:
1755-31
Alexa Fluor® 647 Anti-Mouse Ly-6A/E antibody for use in flow cytometry, immunohistochemistry, and western blot assays.
$219.00
| Clone | D7 |
|---|---|
| Isotype | Rat (Lewis) IgG2aκ |
| Isotype Control | Rat IgG2a-AF647 (KLH/G2a-1-1) |
| Specificity | Mouse Ly-6A/E (Ly-6A.2 and Ly-6E.1) |
| Alternative Names | Sca-1 |
| Description | Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with the monoclonal antibody D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. |
| Immunogen | IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L |
| Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Flow Cytometry – Quality tested 1,3-7 Immunohistochemistry-Frozen Sections – Reported in literature 3 Immunohistochemistry-Paraffin Sections – Reported in literature 2 Immunoprecipitation – Reported in literature 1,3 Western Blot – Reported in literature 1,3 Costimulation – Reported in literature 4 |
| RRID Number | AB_2795254 |
| Gene ID | 110454 (Mouse) 17069 (Mouse) |
| Gene ID Symbol | Ly6a (Mouse) Ly6e (Mouse) |
| Gene ID Aliases | TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1 Ly67; RIG-E; Sca-2; TSA-1; Tsa1 |
| UniProt ID | P05533 (Mouse Q64253 (Mouse |
| UniProt Name | LY6A_MOUSE (Mouse) LY6E_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Ortega G, Korty PE, Shevach EM, Malek TR. Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity. J Immunol. 1986;137:3240-6. (Immunogen, FC, IP, WB)
- 2. van Bragt MP, Ciliberti N, Stanford WL, de Rooij DG, van Pelt AM. LY6A/E (SCA-1) expression in the mouse testis. Biol Reprod. 2005;73:634-8. (IHC-PS)
- 3. English A, Kosoy R, Pawlinski R, Bamezai A. A monoclonal antibody against the 66-kDa protein expressed in mouse spleen and thymus inhibits Ly-6A.2-dependent cell-cell adhesion. J Immunol. 2000;165:3763-71. (FC, IP, WB, IHC-FS)
- 4. Codias EK, Malek TR. Regulation of B lymphocyte responses to IL-4 and IFN-γ by activation through Ly-6A/E molecules. J Immunol. 1990;144:2197-204. (Costim, FC)
- 5. George J, Afek A, Abashidze A, Shmilovich H, Deutsch V, Kopolovich J, et al. Transfer of endothelial progenitor and bone marrow cells influences atherosclerotic plaque size and composition in apolipoprotein E knockout mice. Arterioscler Thromb Vasc Biol. 2005;25:2636-41. (FC)
- 6. Pevsner-Fischer M, Morad V, Cohen-Sfady M, Rousso-Noori L, Zanin-Zhorov A, Cohen S, et al. Toll-like receptors and their ligands control mesenchymal stem cell functions. Blood. 2007;109:1422-32. (FC)
- 7. Filip S, Mokrý J, Vávrová J, Šinkorová Z, Mičuda S, Šponer P, et al. The peripheral chimerism of bone marrow-derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice. J Cell Mol Med. 2014;18:832-43. (FC)
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