My Cart
$0.00
0

Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

  • Home
  • Rat Anti-Mouse Lambda-AP (JC5-1)

Rat Anti-Mouse Lambda-AP (JC5-1)

Cat. No.:
1175-04
AP Anti-Mouse Lambda antibody for use in ELISA, immunohistochemistry, and western blot assays.
$253.00
Size Price (USD) Quantity
1.0 mL $253.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone JC5-1
Isotype Rat IgG2bκ
Isotype Control Rat IgG2b-AP (KLH/G2b-1-2)
Specificity Mouse Lambda
Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 1-14
ELISA – Quality tested
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 15,16
Western Blot-Non-Reducing 18
Separation – Reported in literature 17

RRID Number AB_2794672
Gene ID 111519 (Mouse)
Gene ID Symbol Igl (Mouse)
Gene ID Aliases Igl-x

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

Citations

  1. 1. Heltemes-Harris L, Liu X, Manser T. Progressive surface B cell antigen receptor down-regulation accompanies efficient development of antinuclear antigen B cells to mature, follicular phenotype. J Immunol. 2004;172:823-33. (FC)
  2. 2. Wang Y, Carter RH. CD19 regulates B cell maturation, proliferation, and positive selection in the FDC zone of murine splenic germinal centers. Immunity. 2005;22:749-61. (FC)
  3. 3. O'Neill SK, Shlomchik MJ, Glant TT, Cao Y, Doodes PD, Finnegan A. Antigen-specific B cells are required as APCs and autoantibody-producing cells for induction of severe autoimmune arthritis. J Immunol. 2005;174:3781-8. (FC)
  4. 4. Bai L, Chen Y, He Y, Dai X, Lin X, Wen R, et al. Phospholipase Cγ2 contributes to light-chain gene activation and receptor editing. Mol Cell Biol. 2007;27:5957-67. (FC)
  5. 5. Lindsley RC, Thomas M, Srivastava B, Allman D. Generation of peripheral B cells occurs via two spatially and temporally distinct pathways. Blood. 2007;109:2521-8. (FC)
  6. 6. Pathak S, Ma S, Trinh L, Lu R. A role for interferon regulatory factor 4 in receptor editing. Mol Cell Biol. 2008;28:2815-24. (FC)
  7. 7. Gross AJ, Lyandres JR, Panigrahi AK, Prak ET, DeFranco AL. Developmental acquisition of the Lyn-CD22-SHP-1 inhibitory pathway promotes B cell tolerance. J Immunol. 2009;182:5382-92. (FC)
  8. 8. Stumhofer JS, Tait ED, Quinn WJ 3rd, Hosken N, Spudy B, Goenka R, et al. A role for IL-27p28 as an antagonist of gp130-mediated signaling. Nat Immunol. 2010;11:1119-26. (FC)
  9. 9. Rayamajhi M, Delgado C, Condon TV, Riches DW, Lenz LL. Lung B cells promote early pathogen dissemination and hasten death from inhalation anthrax. Mucosal Immunol. 2012;5:444-54. (FC)
  10. 10. Andrews SF, Zhang Q, Lim S, Li L, Lee J, Zheng N, et al. Global analysis of B cell selection using an immunoglobulin light chain-mediated model of autoreactivity. J Exp Med. 2013;210:125-42. (FC)
  11. 11. Peng C, Eckhardt LA. Role of the Igh intronic enhancer Eμ in clonal selection at the pre-B to immature B cell transition. J Immunol. 2013;191:4399-411. (FC)
  12. 12. Nechanitzky R, Akbas D, Scherer S, Györy I, Hoyler T, Ramamoorthy S, et al. Transcription factor EBF1 is essential for the maintenance of B cell identity and prevention of alternative fates in committed cells. Nat Immunol. 2013;14:867-75. (FC)
  13. 13. Lee E, Liang Q, Ali H, Bayliss L, Beasley A, Bloomfield-Gerdes T, et al. Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery. Nat Biotechnol. 2014;32:356-63. (FC)
  14. 14. Hu J, Oda SK, Shotts K, Donovan EE, Strauch P, Pujanauski LM, et al. Lysophosphatidic acid receptor 5 inhibits B cell antigen receptor signaling and antibody response. J Immunol. 2014;193:85-95. (FC)
  15. 15. Källberg E, Leanderson T. A subset of dendritic cells express joining chain (J-chain) protein. Immunology. 2008;123:590-9. (IHC-FS)
  16. 16. Swanson CL, Wilson TJ, Strauch P, Colonna M, Pelanda R, Torres RM. Type I IFN enhances follicular B cell contribution to the T cell-independent antibody response. J Exp Med. 2010;207:1485-1500. (IHC-FS)
  17. 17. Xiang Y, Garrard WT. The downstream transcriptional enhancer, Ed, positively regulates mouse Ig κ gene expression and somatic hypermutation. J Immunol. 2008;180:6725-32. (Sep)
  18. 18. SouthernBiotech unpublished data (WB-NR)
See All References

Related Products

Related Products

Related Products
Check items to add to the cart or
  1. ELISA plate was coated with Mouse Anti-Rat IgG<sub>1</sub>-UNLB (SB Cat. No. 3060-01), Mouse Anti-Rat IgG<sub>2a</sub>-UNLB (SB Cat. No. 3065-01), Mouse Anti-Rat IgG<sub>2b</sub>-UNLB (SB Cat. No. 3070-01), Mouse Anti-Rat IgG<sub>2c</sub>-UNLB (SB Cat. No. 3075-01), and Mouse Anti-Rat IgM-UNLB (SB Cat. No. 3080-01). Serially diluted Rat IgG<sub>2b</sub>-AP (SB Cat. No. 0118-04) was captured and quantified.
    Rat IgG2b-AP (KLH/G2b-1-2)
    0118-04
    $253.00
  2. ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Lambda-AP (SB Cat. No. 1060-04).
    Goat Anti-Mouse Lambda-AP
    1060-04
    $231.00
  3. ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Rat Anti-Mouse Kappa-AP (SB Cat. No. 1170-04).
    Rat Anti-Mouse Kappa-AP (187.1)
    1170-04
    $253.00
  4. ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Rat Anti-Mouse Kappa-AP (SB Cat. No. 1180-04).
    Rat Anti-Mouse Kappa-AP (H139-52.1)
    1180-04
    $253.00
  5. ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-AP (SB Cat. No. 1030-04).
    Goat Anti-Mouse IgG, Human ads-AP
    1030-04
    $160.00
  6. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
    0100-01
    $45.00
  7. ELISA plate was coated with purified mouse IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgA-AP (SB Cat. No. 1040-04).
    Goat Anti-Mouse IgA-AP
    1040-04
    $159.00
  8. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-AP (SB Cat. No. 1090-04).
    Goat Anti-Mouse IgG2b, Human ads-AP
    1090-04
    $164.00
  9. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-AP (SB Cat. No. 1070-04).
    Goat Anti-Mouse IgG1, Human ads-AP
    1070-04
    $164.00
  10. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>, Human ads-AP (SB Cat. No. 1100-04).
    Goat Anti-Mouse IgG3, Human ads-AP
    1100-04
    $164.00
  11. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-AP (SB Cat. No. 1080-04).
    Goat Anti-Mouse IgG2a, Human ads-AP
    1080-04
    $164.00
  12. ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-AP (SB Cat. No. 1020-04).
    Goat Anti-Mouse IgM, Human ads-AP
    1020-04
    $185.00