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Rat Anti-Mouse Kappa-AP (H139-52.1)
Cat. No.:
1180-04
AP Anti-Mouse Kappa antibody for use in ELISA, immunocytochemistry, and western blot assays.
$253.00
| Clone | H139-52.1 |
|---|---|
| Isotype | Rat (LOU/Ws1) IgG1κ |
| Isotype Control | Rat IgG1-AP (KLH/G1-2-2) |
| Specificity | Mouse Kappa |
| Conjugate | AP (Alkaline Phosphatase) |
| Buffer Formulation | 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0 |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications |
Flow Cytometry – Quality tested 2,5-8 ELISA – Quality tested 2-4 FLISA – Quality tested Immunocytochemistry – Reported in literature 8 Western Blot 9 |
| RRID Number | AB_2794679 |
| Gene ID |
243469 (Mouse) |
| Gene ID Symbol |
Igk (Mouse) |
| Gene ID Aliases | kappa |
| UniProt ID |
P01837 (Mouse |
| UniProt Name |
IGKC_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Labit C, Pierres M. Rat monoclonal antibodies to mouse IgG1, IgG2a, IgG2b, and IgG3 subclasses, and kappa chain isotypic determinants. Hybridoma. 1984;3:163-9. (Immunogen)
- 2. Mitchell DA, Pickering MC, Warren J, Fossati-Jimack L, Cortes-Hernandez J, Cook HT, et al. C1q deficiency and autoimmunity: the effects of genetic background on disease expression. J Immunol. 2002;168:2538-43. (ELISA, FC)
- 3. Kamala T. Hock immunization: A humane alternative to mouse footpad injections. J Immunol Methods. 2007;328:204-14. (ELISA)
- 4. Heidari Y, Fossati-Jimack L, Carlucci F, Walport MJ, Cook HT, Botto M. A lupus-susceptibility C57BL/6 locus on chromosome 3 (Sle18) contributes to autoantibody production in 129 mice. Genes Immun. 2009;10:47-55. (ELISA)
- 5. Pathak S, Ma S, Trinh L, Lu R. A role for interferon regulatory factor 4 in receptor editing. Mol Cell Biol. 2008;28:2815-24. (FC)
- 6. Ma S, Pathak S, Trinh L, Lu R. Interferon regulatory factors 4 and 8 induce the expression of Ikaros and Aiolos to down-regulate pre-B-cell receptor and promote cell-cycle withdrawal in pre-B-cell development. Blood. 2008;111:1396-403. (FC)
- 7. Ma S, Pathak S, Mandal M, Trinh L, Clark MR, Lu R. Ikaros and Aiolos inhibit pre-B-cell proliferation by directly suppressing c-Myc expression. Mol Cell Biol. 2010;30:4149-58. (FC)
- 8. Soni C, Wong EB, Domeier PP, Khan TN, Satoh T, Akira S, et al. B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. J Immunol. 2014;193:4400-14. (ICC)
- 9. SouthernBiotech unpublished data (WB)
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