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Rat Anti-Mouse IL-10-LE/AF (JES5-2A5)
Cat. No.:
10210-14
Low Endotoxin/Azide Free Anti-Mouse IL-10 antibody for use in flow cytometry, immunohistochemistry, western blot, immunoprecipitation, ELISA, ELISpot, multiplex, and neutralization assays.
$275.00
| Clone | JES5-2A5 |
|---|---|
| Isotype | Rat IgG1κ |
| Isotype Control | Rat IgG1-LE/AF (KLH/G1-2-2) |
| Specificity | Mouse IL-10 |
| Alternative Names | Interleukin-10, B cell derived T cell growth factor, B-TCGF, T cell growth inhibitory factor, TGIF, cytokine synthesis inhibitory factor, CSIF |
| Immunogen | E. coli-expressed mouse IL-10 |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications | ELISA-Capture – Quality tested 1-7 ELISA-Detection – Quality tested ELISpot-Capture – Reported in literature 5,6,8 Flow Cytometry – Reported in literature 9 Immunohistochemsitry-Frozen Sections – Reported in literature 10-13 Immunohistochemsitry-Paraffin Sections – Reported in literature 14,15 Western Blot – Reported in literature 9,16,17 Immunoprecipitation – Reported in literature 1 Neutralization – Reported in literature 1,2,18-24 Multiplex-Capture – Reported in literature 3 |
| RRID Number | AB_2794236 |
| Gene ID | 16153 (Mouse) |
| Gene ID Symbol | Il10 (Mouse) |
| Gene ID Aliases | CSIF; Il-10 |
| UniProt ID | P18893 (Mouse |
| UniProt Name | IL10_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Sander B, Höidén I, Andersson U, Möller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993;166:201-14. (Immunogen, ELISA-Capture, IP, Neut)
- 2. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
- 3. Carson RT, Vignali DA. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999;227:41-52. (ELISA-Capture, Multiplex-Capture)
- 4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
- 5. Pack CD, Cestra AE, Min B, Legge KL, Li L, Caprio-Young JC, et al. Neonatal exposure to antigen primes the immune system to develop responses in various lymphoid organs and promotes bystander regulation of diverse T cell specificities. J Immunol. 2001;167:4187-95. (ELISA-Capture, ELISPOT-Capture)
- 6. Frossard CP, Steidler L, Eigenmann PA. Oral administration of an IL-10-secreting Lactococcus lactis strain prevents food-induced IgE sensitization. J Allergy Clin Immunol. 2007;119:952-9. (ELISA-Capture, ELISPOT-Capture)
- 7. da Fonseca DM, Silva CL, Wowk PF, Paula MO, Ramos SG, Horn C, et al. Mycobacterium tuberculosis culture filtrate proteins plus CpG Oligodeoxynucleotides confer protection to Mycobacterium bovis BCG-primed mice by inhibiting interleukin-4 secretion. Infect Immun. 2009;77:5311-21. (ELISA-Capture)
- 8. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
- 9. Weber-Nordt RM, Meraz MA, Schreiber RD. Lipopolysaccharide-dependent induction of IL-10 receptor expression on murine fibroblasts. J Immunol. 1994;153:3734-44. (FC, WB)
- 10. Morris MM, Dyson H, Baker D, Harbige LS, Fazakerley JK, Amor S. Characterization of the cellular and cytokine response in the central nervous system following Semliki Forest virus infection. J Neuroimmunol. 1997;74:185-97. (IHC-FS)
- 11. Hersmann GH, Kriegsmann J, Simon J, Hüttich C, Bräuer R. Expression of cell adhesion molecules and cytokines in murine antigen-induced arthritis. Cell Adhes Commun. 1998;6:69-82. (IHC-FS)
- 12. Issazadeh S, Abdallah K, Chitnis T, Chandraker A, Wells AD, Turka LA, et al. Role of passive T-cell death in chronic experimental autoimmune encephalomyelitis. J Clin Invest. 2000;105:1109-16. (IHC-FS)
- 13. Saba K, Denda-Nagai K, Irimura T. A C-type lectin MGL1/CD301a plays an anti-inflammatory role in murine experimental colitis. Am J Pathol. 2009;174:144-52. (IHC-FS)
- 14. Whiteland JL, Shimeld C, Nicholls SM, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of cytokines in paraffin-embedded mouse tissues. J Immunol Methods. 1997;210:103-8. (IHC-PS)
- 15. Romero-Trevejo JL, Gómez-Villamandos JC, Pedrera M, Blanco A, Bautista MJ, Sánchez-Cordón PJ. Immunohistochemical study of macrophage and cytokine dynamics in the gut of scrapie-infected mice. Histol Histopathol. 2010;25:1025-38. (IHC-PS)
- 16. van der Poll T, Marchant A, Buurman WA, Berman L, Keogh CV, Lazarus DD, et al. Endogenous IL-10 protects mice from death during septic peritonitis. J Immunol. 1995;155:5397-401. (WB)
- 17. Avesani L, Marconi G, Morandini F, Albertini E, Bruschetta M, Bortesi L, et al. Stability of Potato virus X expression vectors is related to insert size: implications for replication models and risk assessment. Transgenic Res. 2007;16:587-97. (WB)
- 18. Gérard C, Bruyns C, Marchant A, Abramowicz D, Vandenabeele P, Delvaux A, et al. Interleukin 10 reduces the release of tumor necrosis factor and prevents lethality in experimental endotoxemia. J Exp Med. 1993;177:547-50. (Neut)
- 19. Reed SG, Brownell CE, Russo DM, Silva JS, Grabstein KH, Morrissey PJ. IL-10 mediates susceptibility to Trypanosoma cruzi infection. J Immunol. 1994;153:3135-40. (Neut)
- 20. Groux H, O'Garra A, Bigler M, Rouleau M, Antonenko S, de Vries JE, et al. A CD4+ T-cell subset inhibits antigen-specific T-cell responses and prevents colitis. Nature. 1997;389:737-42. (Neut)
- 21. Maguire HC Jr, Ketcha KA, Lattime EC. Neutralizing anti-IL-10 antibody upregulates the induction and elicitation of contact hypersensitivity. J Interferon Cytokine Res. 1997;17:763-8. (Neut)
- 22. Osborne J, Devaney E. Interleukin-10 and antigen-presenting cells actively suppress Th1 cells in BALB/c mice infected with the filarial parasite Brugia pahangi. Infect Immun. 1999;67:1599-605. (Neut)
- 23. Pedrosa J, Saunders BM, Appelberg R, Orme IM, Silva MT, Cooper AM. Neutrophils play a protective nonphagocytic role in systemic Mycobacterium tuberculosis infection of mice. Infect Immun. 2000;68:577-83. (Neut)
- 24. Duell BL, Carey AJ, Tan CK, Cui X, Webb RI, Totsika M, et al. Innate transcriptional networks activated in bladder in response to uropathogenic Escherichia coli drive diverse biological pathways and rapid synthesis of IL-10 for defense against bacterial urinary tract infection. J Immunol. 2012;188:781-92. (Neut)
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