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Rat Anti-Mouse IL-10-LE/AF (JES5-16E3)
Cat. No.:
10211-14
Low Endotoxin/Azide Free Anti-Mouse IL-10 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, ELISA, ELISpot, neutralization assays.
$275.00
| Clone | JES5-16E3 |
|---|---|
| Isotype | Rat IgG2bκ |
| Isotype Control | Rat IgG2b-LE/AF (KLH/G2b-1-2) |
| Specificity | Mouse IL-10 |
| Alternative Names | Interleukin-10, B cell derived T cell growth factor, B-TCGF, T cell growth inhibitory factor, TGIF, cytokine synthesis inhibitory factor, CSIF |
| Immunogen | E. coli-expressed mouse IL-10 |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications |
ELISA-Detection – Quality tested 2 ELISA-Capture – Quality tested Flow Cytometry – Quality tested 3-6 ELISpot-Detection – Reported in literature 2 Immunohistochemsitry-Frozen Sections – Reported in literature 4,7-9 Immunocytochemistry – Reported in literature 1 Immunoprecipitation – Reported in literature 1 Neutralization – Reported in literature 1 |
| RRID Number | AB_2794248 |
| Gene ID |
16153 (Mouse) |
| Gene ID Symbol |
Il10 (Mouse) |
| Gene ID Aliases | CSIF; Il-10 |
| UniProt ID |
P18893 (Mouse |
| UniProt Name |
IL10_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Sander B, Höidén I, Andersson U, Möller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993;166:201-14. (Immunogen, ICC, IP, Neut)
- 2. Pack CD, Cestra AE, Min B, Legge KL, Li L, Caprio-Young JC, et al. Neonatal exposure to antigen primes the immune system to develop responses in various lymphoid organs and promotes bystander regulation of diverse T cell specificities. J Immunol. 2001;167:4187-95. (ELISA-Detection, ELISPOT-Detection)
- 3. Groux H, O'Garra A, Bigler M, Rouleau M, Antonenko S, de Vries JE, et al. A CD4+ T-cell subset inhibits antigen-specific T-cell responses and prevents colitis. Nature. 1997;389:737-42. (FC)
- 4. Khanna A, Morelli AE, Zhong C, Takayama T, Lu L, Thomson AW. Effects of liver-derived dendritic cell progenitors on Th1- and Th2-like cytokine responses in vitro and in vivo. J Immunol. 2000;164:1346-54. (FC, IHC-FS)
- 5. Hamada H, Garcia-Hernandez Mde L, Reome JB, Misra SK, Strutt TM, McKinstry KK, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009;182:3469-81. (FC)
- 6. Le Huu D, Matsushita T, Jin G, Hamaguchi Y, Hasegawa M, Takehara K, et al. Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease. Blood. 2013;121:3274-83. (FC)
- 7. Hunter CA, Litton MJ, Remington JS, Abrams JS. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 1994;170:939-45. (IHC-FS)
- 8. Martinelli TM, Van Driel IR, Alderuccio F, Gleeson PA, Toh B. Analysis of mononuclear cell infiltrate and cytokine production in murine autoimmune gastritis. Gastroenterology. 1996;110:1791-802. (IHC-FS)
- 9. Sallinen K, Veräjänkorva E, Pöllänen P. Expression of antigens involved in the presentation of lipid antigens and induction of clonal anergy in the female reproductive tract. J Reprod Immunol. 1999;46:91-101. (IHC-FS)
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