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Rat Anti-Mouse IgM-AF647 (1B4B1)
Cat. No.:
1140-31
Alexa Fluor® 647 Anti-Mouse IgM antibody for use in flow cytometry, immunohistochemistry, and western blotting assays.
$215.00
| Clone | 1B4B1 |
|---|---|
| Isotype | Rat IgG2bκ |
| Isotype Control | Rat IgG2b-AF647 (KLH/G2b-1-2) |
| Specificity | Mouse IgM |
| Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications |
Flow Cytometry – Quality tested 1-6 ELISA – Quality tested 7-10 FLISA – Quality tested Immunohistochemistry-Frozen Sections – Reported in literature 11-15 Western Blot 19 ELISpot – Reported in literature 16 Proximity Ligation Assay – Reported in literature 17 Surface Plasmon Resonance – Reported in literature 18 |
| RRID Number | AB_2895725 |
| Gene ID |
16019 (Mouse) |
| Gene ID Symbol |
Ighm (Mouse) |
| Gene ID Aliases | AI326478; Igh-6; Igh-M; Igh6; Igm; muH |
| UniProt ID |
P01872 (Mouse |
| UniProt Name |
IGHM_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Arens R, Tesselaar K, Baars PA, van Schijndel GM, Hendriks J, Pals ST, et al. Constitutive CD27/CD70 interaction induces expansion of effector-type T cells and results in IFNγ-mediated B cell depletion. Immunity. 2001;15:801-12. (FC)
- 2. Cook AJ, Oganesian L, Harumal P, Basten A, Brink R, Jolly CJ. Reduced switching in SCID B cells is associated with altered somatic mutation of recombined S regions. J Immunol. 2003;171:6556-64. (FC)
- 3. Cariappa A, Boboila C, Moran ST, Liu H, Shi HN, Pillai S. The recirculating B cell pool contains two functionally distinct, long-lived, posttransitional, follicular B cell populations. J Immunol. 2007;179:2270-81. (FC)
- 4. Szyszko EA, Skarstein K, Jonsson R, Brokstad KA. Distinct phenotypes of plasma cells in spleen and bone marrow of autoimmune NOD.B10.H2b mice. Autoimmunity. 2011;44:415-26. (FC)
- 5. Andrews SF, Zhang Q, Lim S, Li L, Lee J, Zheng N, et al. Global analysis of B cell selection using an immunoglobulin light chain-mediated model of autoreactivity. J Exp Med. 2013;210:125-42. (FC)
- 6. Gallagher MP, Shrestha A, Magee JM, Wesemann DR. Detection of true IgE-expressing mouse B lineage cells. J Vis Exp. 2014;94:e52264. (FC)
- 7. Qin L, Greenland JR, Moriya C, Cayabyab MJ, Letvin NL. Effects of type I interferons on the adjuvant properties of plasmid granulocyte-macrophage colony-stimulating factor in vivo. J Virol. 2007;81:10606-13. (ELISA)
- 8. Parker MJ, Xue S, Alexander JJ, Wasserfall CH, Campbell-Thompson ML, Battaglia M, et al. Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice. Diabetes. 2009;58:2277-84. (ELISA)
- 9. Huston MW, van Til NP, Visser TP, Arshad S, Brugman MH, Cattoglio C, et al. Correction of murine SCID-X1 by lentiviral gene therapy using a codon-optimized IL2RG gene and minimal pretransplant conditioning. Mol Ther. 2011;19:1867-77. (ELISA)
- 10. Chen M, Hong MJ, Sun H, Wang L, Shi X, Gilbert BE, et al. Essential role for autophagy in the maintenance of immunological memory against influenza infection. Nat Med. 2014;20:503-10. (ELISA)
- 11. Amano H, Amano E, Moll T, Marinkovic D, Ibnou-Zekri N, Martinez-SorÃa E, et al. The Yaa mutation promoting murine lupus causes defective development of marginal zone B cells. J Immunol. 2003;170:2293-301. (IHC-FS)
- 12. Rempel SA, Hawley RC, Gutiérrez JA, Mouzon E, Bobbitt KR, Lemke N, et al. Splenic and immune alterations of the Sparc-null mouse accompany a lack of immune response. Genes Immun. 2007;8:262-74. (IHC-FS)
- 13. Berretta F, St-Pierre J, Piccirillo CA, Stevenson MM. IL-2 contributes to maintaining a balance between CD4+Foxp3+ regulatory T cells and effector CD4+ T cells required for immune control of blood-stage malaria infection. J Immunol. 2011;186:4862-71. (IHC-FS)
- 14. Khoo LH, Thiam CH, Soh SY, Angeli V. Splenic extrafollicular reactions and BM plasma cells sustain IgM response associated with hypercholesterolemia. Eur J Immunol. 2015;45:1300-12. (IHC-FS)
- 15. A-Gonzalez N, Guillen JA, Gallardo G, Diaz M, de la Rosa JV, Hernandez IH, et al. The nuclear receptor LXRα controls the functional specialization of splenic macrophages. Nat Immunol. 2013;14:831-9. (IHC-FS)
- 16. Mayne CG, Amanna IJ, Nashold FE, Hayes CE. Systemic autoimmunity in BAFF-R-mutant A/WySnJ strain mice. Eur J Immunol. 2008;38:587-98. (ELISPOT)
- 17. Kläsener K, Maity PC, Hobeika E, Yang J, Reth M. B cell activation involves nanoscale receptor reorganizations and inside-out signaling by Syk. Elife. 2014;3:e02069. (Proximity ligation assay)
- 18. Sun Y, Peng I, Senger K, Hamidzadeh K, Reichelt M, Baca M, et al. Critical role of activation induced cytidine deaminase in experimental autoimmune encephalomyelitis. Autoimmunity. 2013;46:157-67. (Surface plasmon resonance)
- 19. SouthernBiotech unpublished data (WB)
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