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  • Rat Anti-Mouse IgG2b-UNLB (SB74g)

Rat Anti-Mouse IgG2b-UNLB (SB74g)

Cat. No.:
1186-01
Purified Anti-Mouse IgG2b antibody for use in ELISA, flow cytometry, immunocytochemistry, and western blot assays.
$182.00
Size Price (USD) Quantity
0.5 mg $182.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone SB74g
Isotype Rat (Sprague Dawley) IgG2bλ
Isotype Control Rat IgG2b-UNLB (KLH/G2b-1-2)
Specificity Mouse IgG2b
Immunogen Mouse IgG2b hybridoma
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested
ELISA – Quality tested 1-3
FLISA – Quality tested
Immunocytochemistry 5
Western Blot 4

RRID Number AB_2794684
Gene ID 16016 (Mouse)
Gene ID Symbol Ighg2b (Mouse)
Gene ID Aliases IgG2b; Igh-3; gamma2b
UniProt ID P01867 (Mouse
UniProt Name IGG2B_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Chen M, Hong MJ, Sun H, Wang L, Shi X, Gilbert BE, et al. Essential role for autophagy in the maintenance of immunological memory against influenza infection. Nat Med. 2014;20:503-10. (ELISA)
  2. 2. de Gouw D, Serra DO, de Jonge MI, Hermans PW, Wessels HJ, Zomer A, et al. The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins. Emerg Microbes Infect. 2014;3:e58. (ELISA)
  3. 3. Zuest R, Valdes I, Skibinski D, Lin Y, Toh YX, Chan K, et al. Tetravalent dengue DIIIC protein together with alum and ODN elicits a Th1 response and neutralizing antibodies in mice. Vaccine. 2015;33:1474-82. (ELISA)
  4. 4. SouthernBiotech unpublished data (WB)
  5. 5. SouthernBiotech published data (ICC)
See All References

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  1. Paraffin embedded AIDS-associated Burkitt lymphoma tissue array was stained with anti-CXCR5 (right) and Rat IgG<sub>2b</sub>-UNLB (SB Cat. No. 0118-01; left) followed by a secondary antibody and Fast Red.<br/>Images from Widney DP, Gui D, Popoviciu LM, Said JW, Breen EC, Huang X, et al. Expression and function of the chemokine, CXCL13, and its receptor, CXCR5, in AIDS-associated non-Hodgkin's lymphoma. AIDS Research and Treatment. 2010;2010:164586. Figure 2(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/3.0/
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  2. ELISA plate was coated with serially diluted Mouse IgG<sub>2b</sub>-UNLB (SB Cat. No. 0104-01). Immunoglobulin was detected with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  3. FLISA plate was coated with purified rat IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG<sub>2b</sub>-CY5 (SB Cat. No. 3070-15).
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  4. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG<sub>1</sub>-UNLB (SB Cat. No. 1144-01) followed by Mouse Anti-Rat IgG<sub>2b</sub>-HRP (SB Cat. No. 3070-05).
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  7. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-UNLB (SB Cat. No. 1080-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  8. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Rat Anti-Mouse IgG<sub>2a</sub>-UNLB (SB Cat. No. 1155-01) followed by Mouse Anti-Rat IgG<sub>1</sub>-HRP (SB Cat. No. 3060-05).
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  9. Spleen, brain, and lung tissues from wild type (+/+) and homozygous Limk2 (-/-) mutant mice were resolved by electrophoresis, transfered to PVDF membrane, and probed with anti-LIMK2 followed by Goat Anti-Rat IgG(H+L), Mouse ads-HRP (SB Cat. No. 3050-05) and chemiluminescent detection.<br/>Image from Rice DS, Hansen GM, Liu F, Crist MJ, Newhouse MM, Potter D, et al. Keratinocyte migration in the developing eyelid requires LIMK2. PLoS One. 2012;7(10):e47168. Figure 1(c)<br/>Reproduced under the Creative Commons Attribution License
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