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Rat Anti-Mouse IgD-LE/AF (11-26)
Cat. No.:
1120-14
Low Endotoxin/Azide Free Anti-Mouse IgD antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, separation, costimulation, and proximity ligation assays.
$325.00
| Clone | 11-26 |
|---|---|
| Isotype | Rat IgG2aκ |
| Isotype Control | Rat IgG2a-LE/AF (KLH/G2a-1-1) |
| Specificity | Mouse IgD |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications |
Flow Cytometry – Quality tested 1-11,14 Immunohistochemistry-Frozen Sections – Reported in literature 10-17 Immunohistochemistry-Paraffin Sections – Reported in literature 23 Immunoprecipitation – Reported in literature 18,19 Separation – Reported in literature 5,20 Costimulation – Reported in literature 21 Proximity Ligation Assay – Reported in literature 22 |
| RRID Number | AB_2794611 |
| Gene ID |
380797 (Mouse) |
| Gene ID Symbol |
Ighd (Mouse) |
| Gene ID Aliases | IgD; Igh-5 |
| UniProt ID |
P01881 (Mouse P01882 (Mouse |
| UniProt Name |
IGHD_MOUSE (Mouse) IGHDM_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Rahman ZS, Rao SP, Kalled SL, Manser T. Normal induction but attenuated progression of germinal center responses in BAFF and BAFF-R signaling-deficient mice. J Exp Med. 2003;198:1157-69. (FC)
- 2. Gorelik L, Cutler AH, Thill G, Miklasz SD, Shea DE, Ambrose C, et al. Cutting edge: BAFF regulates CD21/35 and CD23 expression independent of its B cell survival function. J Immunol. 2004;172:762-6. (FC)
- 3. Cariappa A, Shoham T, Liu H, Levy S, Boucheix C, Pillai S. The CD9 tetraspanin is not required for the development of peripheral B cells or for humoral immunity. J Immunol. 2005;175:2925-30. (FC)
- 4. Erlandsson L, Licence S, Gaspal F, Lane P, Corcoran AE, Mårtensson I. Both the pre-BCR and the IL-7Rα are essential for expansion at the pre-BII cell stage in vivo. Eur J Immunol. 2005;35:1969-76. (FC)
- 5. Mattsson N, Duzevik EG, Pelsue SC. Expansion of CD22lo B cells in the spleen of autoimmune-prone flaky skin mice. Cell Immunol. 2005;234:124-32. (FC)
- 6. Klein U, Casola S, Cattoretti G, Shen Q, Lia M, Mo T, et al. Transcription factor IRF4 controls plasma cell differentiation and class-switch recombination. Nat Immunol. 2006;7:773-82. (FC, Sep)
- 7. Cariappa A, Boboila C, Moran ST, Liu H, Shi HN, Pillai S. The recirculating B cell pool contains two functionally distinct, long-lived, posttransitional, follicular B cell populations. J Immunol. 2007;179:2270-81. (FC)
- 8. Xiang Y, Garrard WT. The downstream transcriptional enhancer, Ed, positively regulates mouse Ig κ gene expression and somatic hypermutation. J Immunol. 2008;180:6725-32. (FC)
- 9. Mayne CG, Amanna IJ, Nashold FE, Hayes CE. Systemic autoimmunity in BAFF-R-mutant A/WySnJ strain mice. Eur J Immunol. 2008;38:587-98. (FC)
- 10. Coffey F, Manser T. Expression of cellular FLIP by B cells is required for their participation in an immune response. J Immunol. 2010;184:4871-9. (FC, IHC-FS)
- 11. Seeley-Fallen MK, Liu LJ, Shapiro MR, Onabajo OO, Palaniyandi S, Zhu X, et al. Actin-binding protein 1 links B-cell antigen receptors to negative signaling pathways. Proc Natl Acad Sci USA. 2014;111:9881-6. (FC, IHC-FS)
- 12. Pasparakis M, Alexopoulou L, Episkopou V, Kollias G. Immune and inflammatory responses in TNFα-deficient mice: a critical requirement for TNFα in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response. J Exp Med. 1996;184:1397-411. (IHC-FS)
- 13. Wu X, Jiang N, Fang Y, Xu C, Mao D, Singh J, et al. Impaired affinity maturation in Cr2-/- mice is rescued by adjuvants without improvement in germinal center development. J Immunol. 2000;165:3119-27. (IHC-FS)
- 14. Rahman ZS, Niu H, Perry D, Wakeland E, Manser T, Morel L. Expression of the autoimmune Fcgr2b NZW allele fails to be upregulated in germinal center B cells and is associated with increased IgG production. Genes Immun. 2007;8:604-12. (FC, IHC-FS)
- 15. Gibb DR, Shikh ME, Kang D, Rowe WJ, El Sayed R, Cichy J, et al. ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo. J Exp Med. 2010;207:623-35. (IHC-FS)
- 16. Simonetti G, Carette A, Silva K, Wang H, De Silva NS, Heise N, et al. IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity. J Exp Med. 2013;210:2887-902. (IHC-FS)
- 17. Soni C, Wong EB, Domeier PP, Khan TN, Satoh T, Akira S, et al. B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. J Immunol. 2014;193:4400-14. (IHC-FS)
- 18. Campbell KS, Cambier JC. B lymphocyte antigen receptors (mIg) are non-covalently associated with a disulfide linked, inducibly phosphorylated glycoprotein complex. EMBO J. 1990;9:441-8. (IP)
- 19. Hutchcroft JE, Harrison ML, Geahlen RL. Association of the 72-kDa protein-tyrosine kinase PTK72 with the B cell antigen receptor. J Biol Chem. 1992;267:8613-9. (IP)
- 20. Bowman EP, Kuklin NA, Youngman KR, Lazarus NH, Kunkel EJ, Pan J, et al. The intestinal chemokine thymus-expressed chemokine (CCL25) attracts IgA antibody-secreting cells. J Exp Med. 2002;195:269-75. (Sep)
- 21. El Shikh ME, El Sayed RM, Szakal AK, Tew JG. T-independent antibody responses to T-dependent antigens: a novel follicular dendritic cell-dependent activity. J Immunol. 2009;182:3482-91. (Costim)
- 22. Kläsener K, Maity PC, Hobeika E, Yang J, Reth M. B cell activation involves nanoscale receptor reorganizations and inside-out signaling by Syk. Elife. 2014;3:e02069. (Proximity ligation assay)
- 23. SouthernBiotech published data (IHC-PS)
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