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Rat Anti-Mouse CD5-AF647 (53-7.3)
Cat. No.:
1547-31
Alexa Fluor® 647 Anti-Mouse CD5 antibody for use in flow cytometry and immunohistochemistry assays.
$219.00
| Clone | 53-7.3 |
|---|---|
| Isotype | Rat (LOU/Ws1/M) IgG2aκ |
| Isotype Control | Rat IgG2a-AF647 (KLH/G2a-1-1) |
| Specificity | Mouse CD5 |
| Alternative Names | Lyt-1, Ly-1, Lymphocyte antigen 1, Ly-12, Ly-A, T1, Tp67 |
| Description | CD5, also known as Lyt-1, is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B-cell antigen CD72. The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. |
| Immunogen | Spleen cells or thymocyte membranes |
| Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Flow Cytometry – Quality tested 1,2 Immunohistochemistry-Frozen Sections – Reported in literature 2 Immunoprecipitation – Reported in literature 1 ELISA – Reported in literature 3 Cytoxicity – Reported in literature 2 Blocking – Reported in literature 4 |
| RRID Number | AB_2794869 |
| Gene ID | 12507 (Mouse) |
| Gene ID Symbol | Cd5 (Mouse) |
| Gene ID Aliases | Ly-1; Ly-12; Ly-A; Lyt-1 |
| UniProt ID | P13379 (Mouse |
| UniProt Name | CD5_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, IP, FC)
- 2. Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980;152:280-95. (FC, IHC-FS)
- 3. Luo W, van de Velde H, von Hoegen I, Parnes JR, Thielemans K. Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72). J Immunol. 1992;148:1630-4. (ELISA)
- 4. Bikah G, Lynd FM, Aruffo AA, Ledbetter JA, Bondada S. A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5. Int Immunol. 1998;10:1185-96. (Block)
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