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Rat Anti-Mouse CD49d-LE/AF (PS/2)
Cat. No.:
1520-14
Low Endotoxin/Azide Free Anti-Mouse CD49d antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, blocking, and adhesion assays.
$248.00
| Clone | PS/2 |
|---|---|
| Isotype | Rat (Fischer) IgG2bκ |
| Isotype Control | Rat IgG2b-LE/AF (KLH/G2b-1-2) |
| Specificity | Mouse/Human CD49d |
| Alternative Names | Integrin α4, VLA-4α, ITGA4, IA4 |
| Description | VLA-4 belongs to the β1-integrin family, which functions both as cell-matrix and cell-cell receptors. As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. In addition to CD44/Pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. CD49d is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. Antibody PS/2 also reacts with human VLA-4. |
| Immunogen | P815 cells |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications | Flow Cytometry – Quality tested 1,4,7,8 Immunohistochemistry-Frozen Sections – Reported in literature 2,4 Immunoprecipitation – Reported in literature 1 Adhesion – Reported in literature 1,3-6 Blocking – Reported in literature 1,4-6 |
| RRID Number | AB_2794790 |
| Gene ID | 16401 (Mouse) 3676 (Human) |
| Gene ID Symbol | Itga4 (Mouse) ITGA4 (Human) |
| Gene ID Aliases | CD49D; Itga4B; IA4 |
| UniProt ID | Q00651 (Mouse P13612 (Human |
| UniProt Name | ITA4_MOUSE (Mouse) ITA4_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Miyake K, Weissman IL, Greenberger JS, Kincade PW. Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. J Exp Med. 1991;173:599-607. (Immunogen, IP, FC, Adhesion, Block)
- 2. Kaplan RN, Riba RD, Zacharoulis S, Bramley AH, Vincent L, Costa C, et al. VEGFR1-positive haematopoietic bone marrow progenitors initiate the pre-metastatic niche. Nature. 2005;438:820-7. (IHC-FS)
- 3. Patrick AL, Rullo J, Beaudin S, Liaw P, Fox-Robichaud AE. Hepatic leukocyte recruitment in response to time-limited expression of TNF-α and IL-1β. Am J Physiol Gastrointest Liver Physiol. 2007;293:G663-72. (Adhesion)
- 4. Miyake K, Medina K, Ishihara K, Kimoto M, Auerbach R, Kincade PW. A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture. J Cell Biol. 1991;114:557-65. (IHC-FS, FC, Adhesion, Block)
- 5. Sasaki K, Pardee AD, Okada H, Storkus WJ. IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit. Eur J Immunol. 2008;38:2865-73. (Adhesion, Block)
- 6. Sasaki K, Zhu X, Vasquez C, Nishimura F, Dusak JE, Huang J, et al. Preferential expression of very late antigen-4 on type 1 CTL cells plays a critical role in trafficking into central nervous system tumors. Cancer Res. 2007;67:6451-58. (Adhesion, Block)
- 7. Qu Y, Taylor JL, Bose A, Storkus WJ. Therapeutic effectiveness of intratumorally-delivered dendritic cells engineered to express the pro-inflammatory cytokine, interleukin IL-32. Cancer Gene Ther. 2011;18:663-73. (FC)
- 8. Banerjee ER, Henderson WR. Defining the molecular role of gp91phox in the immune manifestation of acute allergic asthma using a preclinical murine model. Clin Mol Allergy. 2012;10:2. (FC)
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