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Rat Anti-Mouse CD11b-AF647 (M1/70)
Cat. No.:
1561-31
Alexa Fluor® 647 Anti-Mouse CD11b antibody for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
$219.00
| Clone | M1/70 |
|---|---|
| Isotype | Rat (Dark Agouti) IgG2bκ |
| Isotype Control | Rat IgG2b-AF647 (KLH/G2b-1-2) |
| Specificity | Mouse/Human/Rhesus/Baboon/Rabbit CD11b |
| Alternative Names | Integrin αM, Mac-1α, Mac-1, Mo1, CR3, C3biR |
| Description | CD11b, the integrin αM subunit, combines with CD18, the integrin β2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells. |
| Immunogen | C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes |
| Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Flow Cytometry – Quality tested 2,5,7-10 Immunohistochemistry-Frozen Sections – Reported in literature 3,4 Immunohistochemistry-Paraffin Sections – Reported in literature 12,13 Immunocytochemistry – Reported in literature 14 Immunoprecipitation – Reported in literature 1,2,7 Blocking – Reported in literature 6 |
| RRID Number | AB_2794925 |
| Gene ID | 16409 (Mouse) 3684 (Human) 714832 (Rhesus) 101004087 (Baboon) 100351865 (Rabbit) |
| Gene ID Symbol | Itgam (Mouse) ITGAM (Human) ITGAM (Rhesus) ITGAM (Baboon) ITGAM (Rabbit) |
| Gene ID Aliases | CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik; MO1A; CD11B; MAC-1; MAC1A; SLEB6 |
| UniProt ID | P05555 (Mouse P11215 (Human |
| UniProt Name | ITAM_MOUSE (Mouse) ITAM_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Springer T, Galfré G, Secher DS, Milstein C. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978;8:539-51. (Immunogen, IP)
- 2. Springer T, Galfré G, Secher DS, Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979;9:301-6. (IP, FC)
- 3. Igarashi T, Miyake K, Hayakawa J, Kawabata K, Ishizaki M, Takahashi H, et al. Apoptotic cell death and regeneration in the newborn retina after irradiation prior to bone marrow transplantation. Current Eye Res. 2007;32:543-53. (IHC-FS)
- 4. Kosuge H, Ishihara T, Haraguchi G, Maejima Y, Okada H, Saiki H, et al. Treatment with telmisartan attenuates graft arteriosclerosis in murine cardiac allografts. J Heart Lung Transplant. 2010;29:562-7. (IHC-FS)
- 5. Ault KA, Springer TA. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981;126:359-64. (FC, Human Reactivity)
- 6. Beller DI, Springer TA, Schreiber RD. Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor. J Exp Med. 1982;156:1000-9. (Block, Human Reactivity)
- 7. Martin A, Le Corre R, Pellen P, Bourel D, Merdrignac G, Genetet B, et al. A monoclonal antibody (3A33) that reacts with a mouse-specific epitope of Mac-1 antigen. Tissue Antigens. 1986;28:15-23. (IP, FC)
- 8. Pevsner-Fischer M, Morad V, Cohen-Sfady M, Rousso-Noori L, Zanin-Zhorov A, Cohen S, et al. Toll-like receptors and their ligands control mesenchymal stem cell functions. Blood. 2007;109:1422-32. (FC)
- 9. Barton RW, Rothlein R, Ksiazek J, Kennedy C. The effect of anti-intercellular adhesion molecule-1 on phorbol-ester-induced rabbit lung inflammation. J Immunol. 1989;143:1278-82. (FC, Rabbit Reactivity)
- 10. Marcondes MC, Flynn C, Huitron-Rezendiz S, Watry DD, Zandonatti M, Fox HS. Early antiretroviral treatment prevents the development of central nervous system abnormalities in simian immunodeficiency virus-infected rhesus monkeys. AIDS. 2009;23:1187-95. (FC, Rhesus Reactivity)
- 11. del Zappo GJ, Frankowski H, Gu Y, Osada T, Kanazawa M, Milner R, et al. Microglial cell activation is a source of metalloproteinase generation during hemorrhagic transformation. J Cereb Blood Flow Metab. 2012;32:919-32. (Baboon Reactivity)
- 12. Sata M, Sugiura S, Yoshizumi M, Ouchi Y, Hirata Y, Nagai R. Acute and chronic smooth muscle cell apoptosis after mechanical vascular injury can occur independently of the Fas-death pathway. Arterioscler Thromb Vasc Biol. 2001;21:1733-7. (IHC-PS)
- 13. Dineen SP, Lynn KD, Holloway SE, Miller AF, Sullivan JP, Shames DS, et al. Vascular endothelial growth factor receptor 2 mediates macrophage infiltration into orthotopic pancreatic tumors in mice. Cancer Res. 2008;68:4340-6. (IHC-PS)
- 14. Mizgerd JP, Horwitz BH, Quillen HC, Scott ML, Doerschuk CM. Effects of CD18 deficiency on the emigration of murine neutrophils during pneumonia. J Immunol. 1999;163:995-9. (ICC)
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