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Rat Anti-Human IL-6-LE/AF (MQ2-13A5)
Cat. No.:
10120-14
Low Endotoxin/Azide Free Anti-Human IL-6 antibody for use in flow cytometry, immunoprecipitation, ELISA, ELISpot, multiplex, and neutralization assays.
$275.00
| Clone | MQ2-13A5 |
|---|---|
| Isotype | Rat IgG1κ |
| Isotype Control | Rat IgG1-LE/AF (KLH/G1-2-2) |
| Specificity | Human/Rhesus/Cynomolgus IL-6 |
| Alternative Names | Interleukin-6, hepatocyte stimulating factor, HSF, hepatocyte growth factor, HGF, B cell stimulating factor-2, BSF-2, BSF2, cytotoxic T cell differentiation factor, CDF, hybridoma/plasmacytoma growth factor, HPGF |
| Immunogen | COS-expressed human IL-6 |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications | ELISA-Capture – Quality tested 3,4 ELISA-Detection – Reported in literature 1,2 ELISpot-Capture – Reported in literature 3,5 Flow Cytometry – Reported in literature 6,7 Immunoprecipitation – Reported in literature 8 Neutralization – Reported in literature 1,9 Multiplex-Capture – Reported in literature 10 Note – May be paired with the biotinylated clone MQ2-39C3 (SB Cat. No. 10121-08) in a sandwich ELISA |
| RRID Number | AB_2794159 |
| Gene ID | 3569 (Human) 705819 (Rhesus) 102138971 (Cynomolgus) |
| Gene ID Symbol | IL6 (Human) IL6 (Rhesus) IL6 (Cynomolgus) |
| Gene ID Aliases | BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; EGK_13861 |
| UniProt ID | P05231 (Human P51494 (Rhesus P79341 (Cynomolgus |
| UniProt Name | IL6_HUMAN (Human) IL6_MACMU (Rhesus) IL6_MACFA (Cynomolgus) |
Documentation
Certificate of Analysis Lookup
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- 1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
- 2. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
- 3. Mäkitalo B, Andersson M, Areström I, Karlén K, Villinger F, Ansari A, et al. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods. 2002;270:85-97. (ELISA-Capture, ELISPOT-Capture, Rhesus & Cynomolgus Reactivity)
- 4. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
- 5. Hansson T, Dannæus A, Klareskog L. Cytokine-producing cells in peripheral blood of children with coeliac disease secrete cytokines with a type 1 profile. Clin Exp Immunol. 1999;116:246-50. (ELISPOT-Capture)
- 6. Keller ET, Zhang J, Yao Z, Qi Y. The impact of chronic estrogen deprivation on immunologic parameters in the ovariectomized rhesus monkey (Macaca mulatta) model of menopause. J Reprod Immunol. 2001;50:41-55. (FC, Rhesus Reactivity)
- 7. Sestak K, McNeal MM, Choi A, Cole MJ, Ramesh G, Alvarez X, et al. Defining T-cell-mediated immune responses in rotavirus-infected juvenile rhesus macaques. J Virol. 2004;78:10258-64. (FC, Rhesus Reactivity)
- 8. Zou J, Morford LA, Chougnet C, Dix AR, Brooks AG, Torres N, et al. Human glioma-induced immunosuppression involves soluble factor(s) that alters monocyte cytokine profile and surface markers. J Immunol. 1999;162:4882-92. (IP)
- 9. Sharma S, Nemeth E, Chen Y, Goodnough J, Huston A, Roodman GD, et al. Involvement of hepcidin in the anemia of multiple myeloma. Clin Cancer Res. 2008;14:3262-7. (Neut)
- 10. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Capture, Cynomolgus Reactivity)
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