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Rat Anti-Human IL-5-BIOT (JES1-5A10)
Cat. No.:
10118-08
Biotin Anti-Human IL-5 antibody for use in flow cytometry, ELISA, and ELISpot assays.
$253.00
| Clone | JES1-5A10 |
|---|---|
| Isotype | Rat IgG2aκ |
| Isotype Control | Rat IgG2a-BIOT (KLH/G2a-1-1) |
| Specificity | Human/Rhesus/Cynomolgus/Baboon/Sooty Mangabey IL-5 |
| Alternative Names | Interleukin-5, eosinophil differentiation factor, EDF, T cell replacing factor-1, TRF-1, eosinophil colony stimulating factor, Eo-CSF, B cell growth factor-2, BCGF-2, B cell differentiation factor for IgM, BCDF-m, IgA enhancing factor, IgA-EF |
| Immunogen | COS-expressed human IL-5 |
| Conjugate | BIOT (Biotin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications | ELISA-Capture – Quality tested ELISA-Detection – Quality tested 1-10 ELISpot-Detection – Reported in literature 4,8,11-14 Flow Cytometry – Reported in literature 6 Neutralization – Reported in literature 1-3 |
| RRID Number | AB_2794153 |
| Gene ID | 3567 (Human) 710622 (Rhesus) 102125522 (Cynomolgus) 100137220 (Baboon) 105599744 (Sooty Mangabey) |
| Gene ID Symbol | IL5 (Human) IL5 (Rhesus) IL5 (Cynomolgus) IL5 (Baboon) IL5 (Sooty Mangabey) |
| Gene ID Aliases | EDF; IL-5; TRF |
| UniProt ID | P05113 (Human P48093 (Rhesus P46685 (Sooty Mangabey |
| UniProt Name | IL5_HUMAN (Human) IL5_MACMU (Rhesus) IL5_CERAT (Sooty Mangabey) |
Documentation
Certificate of Analysis Lookup
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- 1. Abrams JS, Silver JE, Van Dyke RE, Gleich GJ. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GM-CSF. In: Gleich GJ, Kay AB, editors. Eosinophils in allergy and inflammation. New York: Marcel Dekker; 1994. p. 133-57. (Immunogen, ELISA-Detection, Neut)
- 2. Bacchetta R, de Waal Malefijt R, Yssel H, Abrams J, de Vries JE, Spits H, et al. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-γ and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990;144:902-8. (ELISA-Detection, Neut)
- 3. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
- 4. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Detection, ELISPOT-Detection)
- 5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
- 6. Krouwels FH, Nocker RE, Snoek M, Lutter R, van der Zee JS, Weller FR, et al. Immunocytochemical and flow cytofluorimetric detection of intracellular IL-4, IL-5 and IFN-γ: applications using blood- and airway-derived cells. J Immunol Methods. 1997;203:89-101. (ELISA-Detection, FC)
- 7. Nyindo M, Kariuki TM, Mola PW, Farah IO, Elson L, Blanton RE, et al. Role of adult worm antigen-specific immunoglobulin E in acquired immunity to Schistosoma mansoni infection in baboons. Infect Immun. 1999;67:636-42. (ELISA-Detection, Baboon Reactivity)
- 8. Mäkitalo B, Andersson M, Areström I, Karlén K, Villinger F, Ansari A, et al. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods. 2002;270:85-97. (ELISA-Detection, ELISPOT-Detection, Rhesus & Cynomolgus Reactivity)
- 9. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Detection)
- 10. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (ELISA-Detection)
- 11. Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4+ T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21. (ELISPOT-Detection)
- 12. Wang Z, Metcalf B, Ribeiro RM, McClure H, Kaur A. Th-1-type cytotoxic CD8+ T-lymphocyte responses to simian immunodeficiency virus (SIV) are a consistent feature of natural SIV infection in sooty mangabeys. J Virol. 2006;80:2771-83. (ELISPOT-Detection, Sooty Mangabey Reactivity)
- 13. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Detection)
- 14. Pichyangkul S, Tongtawe P, Kum-Arb U, Yongvanitchit K, Gettayacamin M, Hollingdale MR, et al. Evaluation of the safety and immunogenicity of Plasmodium falciparum apical membrane antigen 1, merozoite surface protein 1 or RTS,S vaccines with adjuvant system AS02A administered alone or concurrently in rhesus monkeys. Vaccine. 2010;28:452-62. (ELISPOT-Detection, Rhesus Reactivity)
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