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Rat Anti-Human IL-4-LE/AF (MP4-25D2)
Cat. No.:
10117-14
Low Endotoxin/Azide Free Anti-Human IL-4 antibody for use in flow cytometry, immunohistochemistry, ELISA, ELISpot, and neutralization assays.
$275.00
| Clone | MP4-25D2 |
|---|---|
| Isotype | Rat IgG1κ |
| Isotype Control | Rat IgG1-LE/AF (KLH/G1-2-2) |
| Specificity | Human/Rhesus/Cynomolgus/Baboon IL-4 |
| Alternative Names | Interleukin-4, T cell growth factor-2, TCGF-2 , Ia inducing factor, IaIF, B cell stimulating factor-1, BSF-1, Hodgkin's cell growth factor, HCGF, mast cell growth factor-2, MCGF-2, macrophage fusion factor, MFF, T cell growth factor-2, TCGF-2, Hodgkin's cell growth factor, HCGF |
| Immunogen | CHO-expressed human IL-4 |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications |
ELISA-Detection – Quality tested 1,2,4,5 ELISA-Capture – Reported in literature 3 ELISpot-Capture – Reported in literature 2,6 ELISpot-Detection – Reported in literature 7 Flow Cytometry – Reported in literature 8-10 Immunohistochemistry-Frozen Sections – Reported in literature 11-13 Neutralization – Reported in literature 1,14,15 Multiplex-Detection – Reported in literature 16 |
| RRID Number | AB_2794151 |
| Gene ID |
3565 (Human) 574281 (Rhesus) 107130068 (Cynomolgus) 100126747 (Baboon) |
| Gene ID Symbol |
IL4 (Human) IL4 (Rhesus) IL4 (Cynomolgus) IL4 (Baboon) |
| Gene ID Aliases | BCGF-1; BCGF1; BSF-1; BSF1; IL-4; EGK_16838 |
| UniProt ID |
P05112 (Human P51492 (Rhesus P79339 (Cynomolgus Q865Y0 (Baboon |
| UniProt Name |
IL4_HUMAN (Human) IL4_MACMU (Rhesus) IL4_MACFA (Cynomolgus) IL4_PAPAN (Baboon) |
Documentation
Certificate of Analysis Lookup
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- 1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
- 2. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Detection, ELISPOT-Capture)
- 3. Nyindo M, Kariuki TM, Mola PW, Farah IO, Elson L, Blanton RE, et al. Role of adult worm antigen-specific immunoglobulin E in acquired immunity to Schistosoma mansoni infection in baboons. Infect Immun. 1999;67:636-42. (ELISA-Capture, Baboon Reactivity)
- 4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
- 5. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Detection)
- 6. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
- 7. Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4+ T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21. (ELISPOT-Detection)
- 8. Koshiba M, Rosin DL, Hayashi N, Linden J, Sitkovsky MV. Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies. Mol Pharmacol. 1999;55:614-24. (FC)
- 9. Bandeira-Melo C, Sugiyama K, Woods LJ, Weller PF. Cutting edge: eotaxin elicits rapid vesicular transport-mediated release of preformed IL-4 from human eosinophils. J Immunol. 2001;166:4813-7. (FC)
- 10. Gansuvd B, Goodwin J, Asiedu CK, Jiang XL, Jargal U, Andrades P, et al. Invariant natural killer T cells from rhesus macaque spleen and peripheral blood are phenotypically and functionally distinct populations. J Med Primatol. 2008;37:1-11. (FC, Rhesus Reactivity)
- 11. Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
- 12. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
- 13. Sparrelid E, Emanuel D, Fehniger T, Andersson U, Andersson J. Interstitial pneumonitis in bone marrow transplant recipients is associated with local production of TH2-type cytokines and lack of T cell-mediated cytotoxicity. Transplantation. 1997;63:1782-9. (IHC-FS)
- 14. Punnonen J, Yssel H, de Vries JE. The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells. J Allergy Clin Immunol. 1997;100:792-801. (Neut)
- 15. Cousins DJ, Lee TH, Staynov DZ. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169:2498-506. (Neut)
- 16. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Detection, Cynomolgus Reactivity)
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