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Rat Anti-Human IL-3-BIOT (BVD3-1F9)
Cat. No.:
10116-08
Biotin Anti-Human IL-3 antibody for use in flow cytometry, immunohistochemistry, ELISA, western blot, and immunoprecipitation assays.
$303.00
| Clone | BVD3-1F9 |
|---|---|
| Isotype | Rat IgG1κ |
| Isotype Control | Rat IgG1-BIOT (KLH/G1-2-2) |
| Specificity | Human IL-3 |
| Alternative Names | Interleukin-3, burst promoting activity, BP/BPA, eosinophil colony stimulating factor, Eo-CSF, hematopoietic cell growth factor, HCGF, mast cell growth factor, MGF, MCGF, multi-colony stimulating factor, multi-CSF, P cell stimulating activity, PCSA |
| Immunogen | Yeast-expressed human IL-3 |
| Conjugate | BIOT (Biotin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications |
ELISA-Detection – Quality tested 1-4 Flow Cytometry – Reported in literature 5,6 Immunohistochemistry-Frozen Sections – Reported in literature 7,8 Western Blot – Reported in literature 1 Immunoprecipitation – Reported in literature 1,2 Note – May be paired with the purified clone BVD8-3G11 (SB Cat. No. 10115-01) in a sandwich ELISA |
| RRID Number | AB_2794148 |
| Gene ID |
3562 (Human) |
| Gene ID Symbol |
IL3 (Human) |
| Gene ID Aliases | IL-3; MCGF; MULTI-CSF |
| UniProt ID |
P08700 (Human |
| UniProt Name |
IL3_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Kaushansky K, Shoemaker SG, Broudy VC, Lin NL, Matous JV, Alderman EM, et al. Structure-function relationships of interleukin-3. An analysis based on the function and binding characteristics of a series of interspecies chimera of gibbon and murine interleukin-3. J Clin Invest. 1992;90:1879-88. (Immunogen, ELISA-Detection, WB, IP)
- 2. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, IP)
- 3. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Detection)
- 4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
- 5. Giuliani N, Morandi F, Tagliaferri S, Colla S, Bonomini S, Sammarelli G, et al. Interleukin-3 (IL-3) is overexpressed by T lymphocytes in multiple myeloma patients. Blood. 2006;107:841-2. (FC)
- 6. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (FC)
- 7. Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
- 8. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
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