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Rabbit F(ab')2 Anti-Rat IgG(H+L)-AP
Cat. No.:
6130-04
Rabbit F(ab')2 Anti-Rat IgG(H+L)-AP antibody for use in ELISA, immunohistochemistry / immunocytochemistry, and western blot assays.
$138.00
| Isotype | Rabbit F(ab')2 IgG |
|---|---|
| Isotype Control | 0112-04 |
| Specificity | Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM |
| Source | Pepsin digest of Rabbit Anti-Rat IgG(H+L) (SB Cat. No. 6180) |
| Cross Adsorption | None; may react with immunoglobulins from other species and the light chains of other rat immunoglobulins |
| Conjugate | AP (Alkaline Phosphatase) |
| Buffer Formulation | 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0 |
| Clonality | Polyclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications |
Quality tested applications for relevant formats include - ELISA FLISA Other referenced applications for relevant formats include - Immunohistochemistry-Frozen Sections 1 Immunohistochemistry-Paraffin Sections 2 Immunocytochemistry 3-6 Western Blot 7-9 |
| RRID Number | AB_2796188 |
Documentation
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- 2. Büller NV, Rosekrans SL, Metcalfe C, Heijmans J, van Dop WA, Fessler E, et al. Stromal Indian hedgehog signaling is required for intestinal adenoma formation in mice. Gastroenterology. 2015;148:170-80. (IHC-PS)
- 3. Poglitsch CL, Thompson NL. Interaction of antibodies with Fc receptors in substrate-supported planar membranes measured by total internal reflection fluorescence microscopy. Biochemistry. 1990;29:248-54. (ICC)
- 4. Sergejeva S, Johansson A, Malmhäll C, Lötvall J. Allergen exposure-induced differences in CD34+ cell phenotype: relationship to eosinophilopoietic responses in different compartments. Blood. 2004;103:1270-7. (ICC)
- 5. Johansson A, Sergejeva S, Sjöstrand M, Lee JJ, Lötvall J. Allergen-induced traffic of bone marrow eosinophils, neutrophils and lymphocytes to airways. Eur J Immunol. 2004;34:3135-45. (ICC)
- 6. Rådinger M, Bossios A, Alm A, Jeurink P, Lu Y, Malmhäll C, et al. Regulation of allergen-induced bone marrow eosinophilopoiesis: role of CD4+ and CD8+ T cells. Allergy. 2007;62:1410-8. (ICC)
- 7. Bumgarner GW, Zampell JC, Nagarajan S, Poloso NJ, Dorn AS, D'Souza MJ, et al. Modified cell ELISA to determine the solubilization of cell surface proteins: Applications in GPI-anchored protein purification. J Biochem Biophys Methods. 2005;64:99-109. (WB)
- 8. Ivanov S, Bozinovski S, Bossios A, Valadi H, Vlahos R, Malmhäll C, et al. Functional relevance of the IL-23-IL-17 axis in lungs in vivo. Am J Respir Cell Mol Biol. 2007;36:442-51. (WB)
- 9. Lässer C, Alikhani VS, Ekström K, Eldh M, Paredes PT, Bossios A, et al. Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages. J Transl Med. 2011;9:9. (WB)
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