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  • Rabbit F(ab')2 Anti-Mouse IgG(H+L), Human ads-BIOT

Rabbit F(ab')2 Anti-Mouse IgG(H+L), Human ads-BIOT

Cat. No.:
6125-08
Rabbit F(ab')2 Anti-Mouse IgG(H+L)-Biotin antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, and immunohistochemistry assays.
$149.00
Size Price (USD) Quantity
0.5 mg $149.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Rabbit F(ab')2 IgG
Isotype Control 0112-08
Specificity Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source Pepsin digest of Rabbit Anti-Mouse IgG(H+L) (SB Cat. No. 6175)
Cross Adsorption Pooled human sera and immunoglobulins; may react with immunoglobulins from other species
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

Other referenced applications for relevant formats include -
Flow Cytometry 1
Immunohistochemistry-Frozen Sections 3
Immunohistochemistry-Paraffin Sections 2
RRID Number AB_2796184

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Liebman MA, Williams BR, Daley KM, Sharon J. Generation and preliminary characterization of an antibody library with preferential reactivity to human colorectal cancer cells as compared to normal human blood cells. Immunol Lett. 2004;91:179-88. (FC)
  2. 2. Lee C, Das B, Lin TL, Grimes C, Zhang X, Lavezzi T, et al. A rare fraction of drug-resistant follicular lymphoma cancer stem cells interacts with follicular dendritic cells to maintain tumourigenic potential. Br J Haematol. 2012;158:79-90. (IHC-PS)
  3. 3. SouthernBiotech published data (IHC-FS)

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  2. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6175-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  5. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat F(ab')<sub>2</sub> Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1032-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
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  6. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6175-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  7. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).
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  8. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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