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  • Rabbit F(ab')2 Anti-Human IgG(H+L)-UNLB

Rabbit F(ab')2 Anti-Human IgG(H+L)-UNLB

Cat. No.:
6000-01
Purified Rabbit F(ab')2 Anti-Human IgG(H+L) antibody for use in ELISA, immunocytochemistry, and western blot assays.
$61.00
Size Price (USD) Quantity
0.5 mg $61.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Rabbit F(ab')2 IgG
Isotype Control Rabbit F(ab')2 IgG-UNLB
Specificity Reacts with the heavy and light chains of human IgG and the light chains of human IgM and IgA
Source Pepsin digest of Rabbit Anti-Human IgG(H+L) (SB Cat. No. 6140)
Cross Adsorption None; may react with immunoglobulins from other species
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-3
FLISA

Other referenced applications for relevant formats include -
Immunocytochemistry 4
Western Blot 5
RRID Number AB_2796082

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Fernandéz-Shaw S, Kennedy SH, Hicks BR, Edmonds K, Starkey PM, Barlow DH. Anti-endometrial antibodies in women measured by an enzyme-linked immunosorbent assay. Hum Reprod. 1996;11:1180-4. (ELISA)
  2. 2. Sugahara KN, Teesalu T, Larmali PP, Kotamraju VR, Agemy L, Greenwald DR, et al. Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs. Science. 2010;328:1031-5. (ELISA)
  3. 3. Janko C, Franz S, Munoz LE, Siebig S, Winkler S, Schett G, et al. CRP/anti-CRP antibodies assembly on the surfaces of cell remnants switches their phagocytic clearance toward inflammation. Front Immunol. 2011;2:70. (ELISA)
  4. 4. Marchand LS, St-Hilaire S, Putman EA, Serve KM, Pfau JC. Mesothelial cell and anti-nuclear autoantibodies associated with pleural abnormalities in an asbestos exposed population of Libby MT. Toxicol Lett. 2012;208:168-73. (ICC)
  5. 5. Merlo A, Tenca C, Fais F, Battini L, Ciccone E, Grossi CE, et al. Inhibitory receptors CD85j, LAIR-1, and CD152 down-regulate immunoglobulin and cytokine production by human B lymphocytes. Clin Diagn Lab Immunol. 2005;12:705-12. (WB)
See All References

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  1. ELISA plate was coated with serially diluted Rabbit F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0112-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG F(ab')<sub>2</sub>-BIOT and Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  2. Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) and Rabbit IgG-UNLB (SB Cat. No. 0111-01; left) followed by Donkey F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF488 (SB Cat. No. 6442-30) and DAPI.
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6175-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  4. ELISA plate was coated with purified human IgG, IgM, and IgA and mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit F(ab')<sub>2</sub> Anti-Human IgG(H+L), Mouse ads-UNLB (SB Cat. No. 6005-01) followed by Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05).
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  5. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-HRP (SB Cat. No. 4054-05).
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  6. Rabbit Anti-Human IgG(H+L)-UNLB
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  7. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit Ig, Human ads-AF555 (SB Cat. No. 4010-32).
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  8. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  9. Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-FITC (SB Cat. No. 6440-02) and DAPI.
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  10. Paraffin embedded human gastric cancer tissue was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human IgG(H+L), Mouse ads-UNLB (SB Cat. No. 6145-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF555 (SB Cat. No. 6440-32) and DAPI.
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  11. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-AP (SB Cat. No. 4049-04).
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  12. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6444-05).
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  13. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-AF647 (SB Cat. No. 4052-31).
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  14. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human IgG-UNLB (SB Cat. No. 2042-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  15. ELISA plate was coated with purified rabbit IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fab, Mouse/Human SP ads-HRP (SB Cat. No. 4058-05).
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  16. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG(H+L), Mouse/Human/Goat/Sheep SP ads-BIOT (SB Cat. No. 4091-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  17. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
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