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  • Rabbit Anti-Rat IgG(H+L), Human ads-BIOT

Rabbit Anti-Rat IgG(H+L), Human ads-BIOT

Cat. No.:
6185-08
Rabbit Anti-Rat IgG(H+L)-Biotin antibody with minimal reactivity to human proteins for use in ELISA and immunohistochemistry assays.
$116.00
Size Price (USD) Quantity
1.0 mg $116.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Rabbit IgG
Isotype Control Rabbit IgG-BIOT
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pooled antisera from rabbits hyperimmunized with rat IgG
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species and the light chains of other rat immunoglobulins
Purification Method Affinity chromatography on rat IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1
FLISA

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 2,3
Immunohistochemistry-Paraffin Sections 4
RRID Number AB_2796263

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Novitzky N, Davison G, Abdulla R, Mowla S. Definition of the variables affecting efficacy of immunodepletion ex vivo of peripheral blood progenitor cell grafts by alemtuzumab (Campath in the bag). Biol Blood Marrow Transplant. 2013;19:1753-9. (ELISA)
  2. 2. Fang Y, Xu C, Fu Y, Holers VM, Molina H. Expression of complement receptors 1 and 2 on follicular dendritic cells is necessary for the generation of a strong antigen-specific IgG response. J Immunol. 1998;160:5273-9. (IHC-FS)
  3. 3. Mao D, Wu X, Deppong C, Friend LD, Dolecki G, Nelson DM, et al. Negligible role of antibodies and C5 in pregnancy loss associated exclusively with C3-dependent mechanisms through complement alternative pathway. Immunity. 2003;19:813-22. (IHC-FS)
  4. 4. SouthernBiotech published data (IHC-PS)
See All References

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  2. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6175-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  3. NIH/Swiss mouse fibroblast cell line 3T3 was stained with Rat Anti-β-Actin-UNLB (right) followed by Donkey Anti-Rat IgG(H+L), Mouse SP ads-BIOT (SB Cat. No. 6430-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  4. Frozen BALB/c mouse intestine tissue was stained with Rat IgG<sub>2a</sub>-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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