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  • Rabbit Anti-Mouse IgG(H+L)-BIOT

Rabbit Anti-Mouse IgG(H+L)-BIOT

Cat. No.:
6170-08
Rabbit Anti-Mouse IgG(H+L)-Biotin antibody for use in ELISA, immunohistochemistry, western blot, immunoprecipitation, and ELISpot assays.
$77.00
Size Price (USD) Quantity
1.0 mg $77.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Rabbit IgG
Isotype Control Rabbit IgG-BIOT
Specificity Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source Pooled antisera from rabbits hyperimmunized with mouse IgG
Cross Adsorption None; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-4
FLISA

Other referenced applications for relevant formats include -
ELISpot 5
Immunohistochemistry-Frozen Sections 6,7
Immunoprecipitation 8
Western Blot 9-11
RRID Number AB_2796244

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Su W, Zhong W, Zhang Y, Xia Z. Synthesized OVA323-339MAP octamers mitigate OVA-induced airway inflammation by regulating Foxp3 T regulatory cells. BMC Immunol. 2012;13:34. (ELISA)
  2. 2. Fu Q, Wei Z, Liu X, Xiao P, Lu Z, Chen Y. Glyceraldehyde-3-phosphate dehydrogenase, an immunogenic Streptococcus equi ssp. zooepidemicus adhesion protein and protective antigen. J Microbiol Biotechnol. 2013;23:579-85. (ELISA)
  3. 3. Imai DM, Feng S, Hodzic E, Barthold SW. Dynamics of connective-tissue localization during chronic Borrelia burgdorferi infection. Lab Invest. 2013;93:900-10. (ELISA)
  4. 4. Li Y, Tu Z, Qian S, Fung JJ, Markowitz SD, Kusner LL, et al. Myeloid-derived suppressor cells as a potential therapy for experimental autoimmune myasthenia gravis. J Immunol. 2014;193:2127-34. (ELISA)
  5. 5. Marques CP, Kapil P, Hinton DR, Hindinger C, Nutt SL, Ransohoff RM, et al. CXCR3-dependent plasma blast migration to the central nervous system during viral encephalomyelitis. J Virol. 2011;85:6136-47. (ELISpot)
  6. 6. Turnberg D, Botto M, Warren J, Morgan BP, Walport MJ, Cook HT. CD59a deficiency exacerbates accelerated nephrotoxic nephritis in mice. J Am Soc Nephrol. 2003;14:2271-9. (IHC-FS)
  7. 7. Biswas PS, Gupta S, Stirzaker RA, Kumar V, Jessberger R, Lu TT, et al. Dual regulation of IRF4 function in T and B cells is required for the coordination of T-B cell interactions and the prevention of autoimmunity. J Exp Med. 2012;209:581-96. (IHC-FS)
  8. 8. Dosch DD, Ballmer-Hofer K. Transmembrane domain-mediated orientation of receptor monomers in active VEGFR-2 dimers. FASEB J. 2010;24:32-8. (IP)
  9. 9. Martin RM, Brady JL, Lew AM. The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice. J Immunol Methods. 1998;212:187-92. (WB)
  10. 10. Duan Y, Huang S, Yang J, Niu P, Gong Z, Liu X, et al. HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo[a]pyrene and interacts with casein kinase 2. Cell Stress Chaperones. 2014;19:271-9. (WB)
  11. 11. Hadzhieva M, Vassilev TL, Roumenina LT, Bayry J, Kaveri SV, Lacroix-Desmazes S, et al. Mechanism and functional implications of the heme-induced binding promiscuity of IgE. Biochemistry. 2015;54:2061-72. (WB)
See All References

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  2. Frozen newborn mouse cartilage sections were blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Mouse Anti-Collagen Type II-UNLB (right) followed by Rabbit F(ab')<sub>2</sub> Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6125-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G<sup>&reg;</sup> Anti-Fade (SB Cat. No. 0100-35).
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  7. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6175-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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