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Mouse IgG2a-LE/AF (HOPC-1)
Cat. No.:
0103-14
Low Endotoxin/Azide Free Mouse IgG1 for use as a control in flow cytometry, immunohistochemistry / immunocytochemistry, western blot, immunoprecipitation, and in vivo / in vitro assays and as a standard in ELISA.
$184.00
| Clone | HOPC-1 |
|---|---|
| Isotype | Mouse IgG2aλ |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications |
Flow Cytometry – Quality tested 6-12 ELISA – Quality tested 1-5 FLISA – Quality tested Immunohistochemistry-Frozen Sections – Reported in literature 13,14 Immunohistochemistry-Paraffin Sections 20 Immunocytochemistry – Reported in literature 15,21 Western Blot – Reported in literature 17 Immunoprecipitation – Reported in literature 15,16 In vitro Control – Reported in literature 7,8,17 In vivo Control – Reported in literature 17,18 |
| RRID Number | AB_2793872 |
Documentation
Certificate of Analysis Lookup
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- 1. Beger E, Deocharan B, Edelman M, Erblich B, Gu Y, Putterman C. A peptide DNA surrogate accelerates autoimmune manifestations and nephritis in lupus-prone mice. J Immunol. 2002;168:3617-26. (ELISA)
- 2. Wang J, Anders RA, Wu Q, Peng D, Cho JH, Sun Y, et al. Dysregulated LIGHT expression on T cells mediates intestinal inflammation and contributes to IgA nephropathy. J Clin Invest. 2004;113:826-35. (ELISA)
- 3. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Igα serine/threonines fine-tune Ig α tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA)
- 4. Kamphuis T, Meijerhof T, Stegmann T, Lederhofer J, Wilschut J, de Haan A. Immunogenicity and protective capacity of a virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice. PLoS One. 2012;7(5):e36812. (ELISA)
- 5. Faustino L, Fonseca DM, Florsheim EB, Resende RR, Lepique AP, Faquim-Mauro E, et al. Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation. Mucosal Immunol. 2014;7:1199-208. (ELISA)
- 6. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC)
- 7. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (FC, in vitro control)
- 8. Cosmi L, Liotta F, Lazzeri E, Francalanci M, Angeli R, Mazzinghi B, et al. Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood. 2003;102:4107-14. (FC, in vitro control)
- 9. Charerntantanakul W, Platt R, Roth JA. Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production. Viral Immunol. 2006;19:646-61. (FC)
- 10. Crescioli C, Cosmi L, Borgogni E, Santarlasci V, Gelmini S, Sottili M, et al. Methimazole inhibits CXC chemokine ligand 10 secretion in human thyrocytes. J Endocrinol. 2007;195:145-55. (FC)
- 11. Wen K, Bui T, Li G, Liu F, Li Y, Kocher J, et al. Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection. Comp Immunol Microbiol Infect Dis. 2012;35:289-301. (FC)
- 12. Kroesen M, Nierkens S, Ansems M, Wassink M, Orentas RJ, Boon L, et al. A transplantable TH-MYCN transgenic tumor model in C57Bl/6 mice for preclinical immunological studies in neuroblastoma. Int J Cancer. 2014;134:1335-45. (FC)
- 13. Chen M, Lam BK, Kanaoka Y, Nigrovic PA, Audoly LP, Austen KF, et al. Neutrophil-derived leukotriene B4 is required for inflammatory arthritis. J Exp Med. 2006;203:837-42. (IHC-FS)
- 14. Balogh A, Pozsgay J, Matkó J, Dong Z, Kim CJ, Várkonyi T, et al. Placental protein 13 (PP13/galectin-13) undergoes lipid raft-associated subcellular redistribution in the syncytiotrophoblast in preterm preeclampsia and HELLP syndrome. Am J Obstet Gynecol. 2011;205:156e1-14. (IHC-FS)
- 15. Guimarães AJ, Hamilton AJ, de M Guedes HL, Nosanchuk JD, Zancopé-Oliveira RM. Biological function and molecular mapping of M antigen in yeast phase of Histoplasma capsulatum. PLoS One. 2008;3(10):e3449. (ICC, IP)
- 16. Lindner JM, Kayo H, Hedlund S, Fukuda Y, Fukao T, Nielsen PJ. Cutting edge: The transcription factor Bob1 counteracts B cell activation and regulates miR-146a in B cells. J Immunol. 2014;192:4483-6. (IP)
- 17. Lighvani S, Baik N, Diggs JE, Khaldoyanidi S, Parmer RJ, Miles LA. Regulation of macrophage migration by a novel plasminogen receptor Plg-RKT. Blood. 2011;118:5622-30. (WB, in vitro control, in vivo control)
- 18. Smith MA, Weingarten RA, Russo LM, Ventura CL, O'Brien AD. Antibodies against hemolysin and cytotoxic necrotizing factor type 1 (CNF1) reduce bladder inflammation in a mouse model of urinary tract infection with toxigenic uropathogenic Escherichia coli. Infect Immun. 2015;83:1661-73. (In vivo control)
- 19. Xia Y, Janda A, Eryilmaz E, Casadevall A, Putterman C. The constant region affects antigen binding of antibodies to DNA by altering secondary structure. Mol Immunol. 2013;56:28-37. (Circular dichroism spectroscopy protein control)
- 20. SouthernBiotech published data (IHC-PS)
- 21. Pilling D, Tucker NM, Gomer RH. Aggregated IgG inhibits the differentiation of human fibrocytes. J Leukoc Biol. 2006;79:1242-51 (ICC)
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