Mouse Anti-Rat IgG2b-FITC (2B10A8)
Cat. No.:
3070-02
FITC Anti-Rat IgG2b antibody for use in flow cytometry, immunohistochemistry, and western blot assays.
$253.00
![FLISA plate was coated with purified rat IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG<sub>2b</sub>-FITC (SB Cat. No. 3070-02).](https://www.southernbiotech.com/media/catalog/product/cache/7149a3d30fe6da616557c86e375dcb18/5/2/5248f88e668213018c8bc18b08d4861d6145731ce5ac1f51e0d3e40c8c22324c.jpeg)
![FLISA plate was coated with purified rat IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG<sub>2b</sub>-FITC (SB Cat. No. 3070-02).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/5/2/5248f88e668213018c8bc18b08d4861d6145731ce5ac1f51e0d3e40c8c22324c.jpeg)
![Frozen mouse spleen tissue was stained with Rat IgG<sub>2b</sub>-UNLB isotype control (SB Cat. No. 0118-01; left) and Rat Anti-Mouse Ly-6G/Ly-6C-UNLB (SB Cat. No. 1900-01; right) followed by Mouse Anti-Rat IgG<sub>2b</sub>-FITC (SB Cat. No. 3070-02) and DAPI.](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/9/f/9f27d1673be6abe765cfc24969507c631d1b952ae1bc0ad9c4792e98c3f8de56.jpeg)
![BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD3ε-UNLB (SB Cat. 1535-01) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09) followed by Mouse Anti-Rat IgG<sub>2b</sub>-FITC (SB Cat. No. 3070-02).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/c/7/c79087ae2e68e9ef1695c3b0f028081ca333fa4936d7379872b2d116079694e3.jpeg)
![BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Mouse Anti-Rat IgG<sub>2b</sub>-FITC (SB Cat. No. 3070-02).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/d/3/d301b4f63a84670d6dd0efc86f0d55046d84673056c67c6ddb0e1729579834d8.jpeg)
Clone | 2B10A8 |
---|---|
Isotype | Mouse (BALB/c) IgG2bκ |
Isotype Control | Mouse IgG2b-FITC (A-1) |
Specificity | Rat IgG2b |
Immunogen | Rat IgG2b hybridoma |
Conjugate | FITC (Fluorescein) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
Clonality | Monoclonal |
Concentration | 0.5 mg/mL |
Volume | 1.0 mL |
Recommended Storage | 2-8°C; Avoid exposure to light |
Applications |
Flow Cytometry – Quality tested 1-4 ELISA – Quality tested 5,6 FLISA – Quality tested Immunohistochemistry-Frozen Sections 7 Western Blot 8 |
RRID Number | AB_2795881 |
UniProt ID |
P20761 (Rat) |
UniProt Name |
IGG2B_RAT (Rat) |
Documentation
Certificate of Analysis Lookup
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- 1. Piaggio E, Mars LT, Cassan C, Cabarrocas J, Hofstätter M, Desbois S, et al. Multimerized T cell epitopes protect from experimental autoimmune diabetes by inducing dominant tolerance. Proc Natl Acad Sci USA. 2007;104:9393-8. (FC)
- 2. Vanden Bush TJ, Buchta CM, Claudio J, Bishop GA. Cutting Edge: Importance of IL-6 and cooperation between innate and adaptive immune receptors in cellular vaccination with B lymphocytes. J Immunol. 2009;183:4833-7. (FC)
- 3. Troeger C, Perahud I, Moser S, Holzgreve W. Transplacental traffic after in utero mesenchymal stem cell transplantation. Stem Cells Dev. 2010;19:1385-92. (FC)
- 4. Ge W, Jiang J, Liu W, Lian D, Saito A, Garcia B, et al. Regulatory T cells are critical to tolerance induction in presensitized mouse transplant recipients through targeting memory T cells. Am J Transplant. 2010;10:1760-73. (FC)
- 5. Du F, Lü L, Fu Q, Dai M, Teng J, Fan W, et al. T-614, a novel immunomodulator, attenuates joint inflammation and articular damage in collagen-induced arthritis. Arthritis Res Ther. 2008;10:R136. (ELISA)
- 6. Pastor M, Esquisabel A, MarquÃnez I, Talavera A, Pedraz JL. Cellulose acetate phthalate microparticles containing Vibrio cholerae: steps toward an oral cholera vaccine. J Drug Target. 2014;22:478-87. (ELISA)
- 7. SouthernBiotech published data
- 8. SouthernBiotech unpublished data
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