Mouse Anti-Rat IgG2a-PE (2A8F4)
Cat. No.:
3065-09
PE Anti-Rat IgG2a antibody for use in flow cytometry assays.
$237.00
![FLISA plate was coated with purified rat IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG<sub>2a</sub>-PE (SB Cat. No. 3065-09).](https://www.southernbiotech.com/media/catalog/product/cache/7149a3d30fe6da616557c86e375dcb18/e/7/e79e91d128063ce8475ae55a77386d815a9a67f61cd56b69bb6e498b40310136.jpeg)
![FLISA plate was coated with purified rat IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG<sub>2a</sub>-PE (SB Cat. No. 3065-09).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/e/7/e79e91d128063ce8475ae55a77386d815a9a67f61cd56b69bb6e498b40310136.jpeg)
![BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD5-UNLB (SB Cat. No. 1547-01) followed by Mouse Anti-Rat IgG<sub>2a</sub>-PE (SB Cat. No. 3065-09).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/2/3/2365b62755bb74f8654a0df8672bd13ac147c1f65188cc81a6f9f54d6631fb39.jpeg)
![BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD19-UNLB (SB Cat. No. 1575-01) and Rat Anti-Mouse CD3ε-FITC (SB Cat. No. 1535-02) followed by Mouse Anti-Rat IgG<sub>2a</sub>-PE (SB Cat. No. 3065-09).](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/f/3/f3e7450c68d07cfe8f76a23a2538af83af0f37d9f16999cdfd9ac4a5ad82d59e.jpeg)
Clone | 2A8F4 |
---|---|
Isotype | Mouse (BALB/c) IgG1κ |
Isotype Control | Mouse IgG1-PE (15H6) |
Specificity | Rat IgG2a |
Immunogen | Rat IgG2a hybridoma |
Conjugate | PE (R-phycoerythrin) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
Clonality | Monoclonal |
Concentration | 0.1 mg/mL |
Volume | 1.0 mL |
Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
Applications |
Flow Cytometry – Quality tested 1-5 ELISA – Quality tested 6 FLISA – Quality tested Immunohistochemistry-Frozen Sections – Reported in literature 7 ELISpot – Reported in literature 8 |
RRID Number | AB_2795875 |
Gene ID |
367586 (Rat) |
Gene ID Symbol |
IgG-2a (Rat) |
UniProt ID |
P20760 (Rat) |
UniProt Name |
IGG2A_RAT (Rat) |
Documentation
Certificate of Analysis Lookup
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- 1. Kim DD, Miwa T, Song W. Retrovirus-mediated over-expression of decay-accelerating factor rescues Crry-deficient erythrocytes from acute alternative pathway complement attack. J Immunol. 2006;117:5558-66. (FC)
- 2. Kim DD, Miwa T, Kimura Y, Schwendener RA, van Lookeren Campagne M, Song WC. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood. 2008;112:1109-19. (FC)
- 3. Troeger C, Perahud I, Moser S, Holzgreve W. Transplacental traffic after in utero mesenchymal stem cell transplantation. Stem Cells Dev. 2010;19:1385-92. (FC)
- 4. Herbst T, Esser J, Prati M, Kulagin M, Stettler R, Zaiss MM, et al. Antibodies and IL-3 support helminth-induced basophil expansion. Proc Natl Acad Sci USA. 2012;109:14954-9. (FC)
- 5. Naik E, Webster JD, DeVoss J, Liu J, Suriben R, Dixit VM. Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X. J Exp Med. 2014;211:1947-55. (FC)
- 6. Du F, Lü L, Fu Q, Dai M, Teng J, Fan W, et al. T-614, a novel immunomodulator, attenuates joint inflammation and articular damage in collagen-induced arthritis. Arthritis Res Ther. 2008;10:R136. (ELISA)
- 7. Birjandi SZ, Ippolito JA, Ramadorai AK, Witte PL. Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol. 2011;186:3441-51. (IHC-FS)
- 8. Sharkar MT, Jou M, Hossain MB, Lönnerdal B, Stephensen CB, Raqib R. Prenatal zinc supplementation of zinc-adequate rats adversely affects immunity in offspring. J Nutr. 2011;141:1559-64. (ELISPOT)
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