My Cart
$0.00
0

Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

  • Home
  • Mouse Anti-Rat IgG2a-AF488 (2A8F4)

Mouse Anti-Rat IgG2a-AF488 (2A8F4)

Cat. No.:
3065-30
Alexa Fluor® 488 Anti-Rat IgG2a antibody for use in flow cytometry and immunohistochemistry assays.
$289.00
Size Price (USD) Quantity
0.1 mg $289.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone 2A8F4
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-AF488 (15H6)
Specificity Rat IgG2a
Immunogen Rat IgG2a hybridoma
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 1-5
ELISA – Quality tested 6
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 7
ELISpot – Reported in literature 8

RRID Number AB_2795877
Gene ID 367586 (Rat)
Gene ID Symbol IgG-2a (Rat)
UniProt ID P20760 (Rat
UniProt Name IGG2A_RAT (Rat)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

Citations

  1. 1. Kim DD, Miwa T, Song W. Retrovirus-mediated over-expression of decay-accelerating factor rescues Crry-deficient erythrocytes from acute alternative pathway complement attack. J Immunol. 2006;117:5558-66. (FC)
  2. 2. Kim DD, Miwa T, Kimura Y, Schwendener RA, van Lookeren Campagne M, Song WC. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood. 2008;112:1109-19. (FC)
  3. 3. Troeger C, Perahud I, Moser S, Holzgreve W. Transplacental traffic after in utero mesenchymal stem cell transplantation. Stem Cells Dev. 2010;19:1385-92. (FC)
  4. 4. Herbst T, Esser J, Prati M, Kulagin M, Stettler R, Zaiss MM, et al. Antibodies and IL-3 support helminth-induced basophil expansion. Proc Natl Acad Sci USA. 2012;109:14954-9. (FC)
  5. 5. Naik E, Webster JD, DeVoss J, Liu J, Suriben R, Dixit VM. Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X. J Exp Med. 2014;211:1947-55. (FC)
  6. 6. Du F, Lü L, Fu Q, Dai M, Teng J, Fan W, et al. T-614, a novel immunomodulator, attenuates joint inflammation and articular damage in collagen-induced arthritis. Arthritis Res Ther. 2008;10:R136. (ELISA)
  7. 7. Birjandi SZ, Ippolito JA, Ramadorai AK, Witte PL. Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol. 2011;186:3441-51. (IHC-FS)
  8. 8. Sharkar MT, Jou M, Hossain MB, Lönnerdal B, Stephensen CB, Raqib R. Prenatal zinc supplementation of zinc-adequate rats adversely affects immunity in offspring. J Nutr. 2011;141:1559-64. (ELISPOT)
See All References

Related Products

Related Products

Related Products
Check items to add to the cart or
  1. FLISA plate was coated with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-UNLB (SB Cat. No. 1070-01), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-UNLB (SB Cat. No. 1080-01), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-UNLB (SB Cat. No. 1090-01), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-UNLB (SB Cat. No. 1100-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG<sub>1</sub>-AF488 (SB Cat. No. 0102-30) was captured and fluorescence intensity quantified.
    Mouse IgG1-AF488 (15H6)
    0102-30
    $196.00
  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
    0100-01
    $45.00
  3. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD22-UNLB (SB Cat. No. 1580-01) followed by Mouse Anti-Rat IgG<sub>1</sub>-AF488 (SB Cat. No. 3061-30).
    Mouse Anti-Rat IgG1-AF488 (G11C5)
    3061-30
    $289.00
  4. FLISA plate was coated with purified rat IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG<sub>2b</sub>-AF488 (SB Cat. No. 3070-30).
    Mouse Anti-Rat IgG2b-AF488 (2B10A8)
    3070-30
    $289.00
  5. ELISA plate was coated with serially diluted Rat IgG<sub>2a</sub>-UNLB (SB Cat. No. 0117-01). Immunoglobulin was detected with Mouse Anti-Rat IgG<sub>1</sub>-BIOT (SB Cat. No. 3061-08), Mouse Anti-Rat IgG<sub>2a</sub>-BIOT (SB Cat. No. 3065-08), Mouse Anti-Rat IgG<sub>2b</sub>-BIMA (SB Cat. No. 3070-28), Mouse Anti-Rat IgG<sub>2c</sub>-BIOT (SB Cat. No. 3075-08), and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Rat IgG2a-UNLB (KLH/G2a-1-1)
    0117-01
    $130.00
  6. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD24-UNLB (SB Cat. No. 1815-01) followed by Mouse Anti-Rat IgG<sub>2c</sub>-FITC (SB Cat. No. 3075-02).
    Mouse Anti-Rat IgG2c-FITC (2C8F1)
    3075-02
    $253.00
  7. FLISA plate was coated with purified rat IgM and IgG. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgM-AF488 (SB Cat. No. 3080-30).
    Mouse Anti-Rat IgM-AF488 (M2A1)
    3080-30
    $289.00
  8. FLISA plate was coated with purified rat IgG<sub>1</sub>κ, IgG<sub>2a</sub>λ, IgG<sub>2a</sub>λ, IgG<sub>2b</sub>κ, IgG<sub>2b</sub>λ, IgG<sub>2c</sub>κ, IgMκ, and IgMλ. Immunoglobulins were detected with serially diluted Mouse Anti-Rat Kappa-FITC (SB Cat. No. 3090-02).
    Mouse Anti-Rat Kappa-FITC (K4F5)
    3090-02
    $363.00