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Mouse Anti-Rat IgG(H+L), Mouse SP ads-UNLB

Cat. No.:
3053-01
Purified Mouse Anti-Rat IgG(H+L) antibody with minimal reactivity to mouse serum proteins for use in ELISA assays.
$99.00
Size Price (USD) Quantity
1.0 mg $99.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Mouse IgG
Isotype Control Mouse IgG-UNLB
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Ascites from mice hyperimmunized with rat IgG
Cross Adsorption Mouse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rat IgG covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2795852

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  2. Donkey Anti-Rat IgG(H+L), Mouse SP ads-UNLB
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  3. Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF647
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  4. ELISA plate was coated with purified rat IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L), Human ads-UNLB (SB Cat. No. 6185-01) followed by Rat Anti-Rabbit Ig-UNLB (SB Cat. No. 4065-05).
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  5. ELISA plate was coated with purified rat IgG and IgM and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rat Ig, Mouse ads-UNLB (SB Cat. No. 3010-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat Anti-Rat Ig, Mouse ads-UNLB
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  6. Purified Human Type I Collagen (SB Cat. No. 1200-01S) was resolved by electrophoresis, transferred to PVDF membrane, probed with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), and visualized using Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05) secondary antibody and chemiluminescent detection.
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  7. ELISA plate was coated with purified rat IgG and IgM, mouse IgG, IgM, and IgA, and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG(H+L), Mouse/Human ads-UNLB (SB Cat. No. 3051-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  8. ELISA plate was coated with serially diluted Rat IgG-UNLB (SB Cat. No. 0108-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  9. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-PE (SB Cat. No. 6300-09).
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  10. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L)-UNLB (SB Cat. No. 6180-01) followed by Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05).
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  11. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-UNLB (SB Cat. No. 3030-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  12. ELISA plate was coated with serially diluted Rat IgM-UNLB (SB Cat. No. 0120-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Goat Anti-Rat IgM-BIOT (SB Cat. No. 3020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  13. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Mouse/Rat SP ads-AP (SB Cat. No. 6421-04).
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  15. ELISA plate was coated with purified rat IgG and IgM and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-UNLB (SB Cat. No. 3052-01) followed by Swine Anti-Goat IgG(H+L), Human/ Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  16. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  17. ELISA plate was coated with purified rat IgG and IgM and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG(H+L), Mouse ads-UNLB (SB Cat. No. 3050-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Rat IgG(H+L), Mouse ads-UNLB
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  18. ELISA plate was coated with purified rat IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Rabbit F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Human ads-UNLB (SB Cat. No. 6135-01) followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05).
    Rabbit F(ab')2 Anti-Rat IgG(H+L), Human ads-UNLB
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