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  • Mouse Anti-Rat IgG(H+L), Mouse SP ads-BIOT

Mouse Anti-Rat IgG(H+L), Mouse SP ads-BIOT

Cat. No.:
3053-08
Mouse Anti-Rat IgG(H+L)-Biotin antibody with minimal reactivity to mouse serum proteins for use in ELISA assays.
$127.00
Size Price (USD) Quantity
1.0 mg $127.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Mouse IgG
Isotype Control Mouse IgG-BIOT
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Ascites from mice hyperimmunized with rat IgG
Cross Adsorption Mouse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rat IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2795853

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG-BIOT (SB Cat. No. 0107-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  2. NIH/Swiss mouse fibroblast cell line 3T3 was stained with Rat Anti-β-Actin-UNLB (right) followed by Donkey Anti-Rat IgG(H+L), Mouse SP ads-BIOT (SB Cat. No. 6430-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Donkey Anti-Rat IgG(H+L), Mouse SP ads-BIOT
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  3. Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Rat Anti-Mouse IgA-UNLB (SB Cat. No. 1165-01) followed by Rabbit Anti-Rat IgG(H+L), Human ads-BIOT (SB Cat. No. 6185-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G<sup>&reg;</sup> Anti-Fade (SB Cat. No. 0100-35).
    Rabbit Anti-Rat IgG(H+L), Human ads-BIOT
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  4. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  5. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 3051-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rat IgG(H+L), Mouse/Human ads-BIOT
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  6. ELISA plate was coated with serially diluted Rat IgG-UNLB (SB Cat. No. 0108-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  7. Frozen rat gastrocnemic muscle section was stained with Goat Anti-Rat IgM-BIOT (SB Cat. No. 3020-08) followed by a CY3 secondary reagent and DAPI.<br/>Image from Duehrkop C, Banz Y, Spirig R, Miescher S, Nolte MW, Spycher M, et al. C1 esterase inhibitor reduces lower extremity ischemia/reperfusion injury and associated lung damage. PLoS One. 2013;8(8):e72059. Figure 2(h)<br/>Reproduced under the Creative Commons Attribution License
    Goat Anti-Rat IgM-BIOT
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  8. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  9. ELISA plate was coated with serially diluted Rat IgM-UNLB (SB Cat. No. 0120-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Goat Anti-Rat IgM-BIOT (SB Cat. No. 3020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  10. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
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  11. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3052-08) and Streptavidin-FITC (SB Cat. No. 7100-02).
    Goat F(ab')2 Anti-Rat IgG(H+L), Mouse ads-BIOT
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  12. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L)-BIOT (SB Cat. No. 6180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Rabbit Anti-Rat IgG(H+L)-BIOT
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  13. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
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  14. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Fab Anti-Rat Ig, Mouse ads-BIOT (SB Cat. No. 3000-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  15. Frozen BALB/c mouse intestine tissue was stained with Rat IgG<sub>2a</sub>-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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