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  • Mouse Anti-Rabbit IgG(H+L), Mouse/Human/Goat/Sheep SP ads-UNLB

Mouse Anti-Rabbit IgG(H+L), Mouse/Human/Goat/Sheep SP ads-UNLB

Cat. No.:
4091-01
Purified Mouse Anti-Rabbit IgG(H+L) antibody with minimal reactivity to mouse, human, goat, and sheep serum proteins for use in ELISA assays.
$72.00
Size Price (USD) Quantity
1.0 mg $72.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Mouse IgG
Isotype Control Mouse IgG-UNLB
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Ascites from mice hyperimmunized with rabbit Ig
Cross Adsorption Mouse, human, goat, and sheep serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796006

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with serially diluted Mouse IgG-UNLB (SB Cat. No. 0107-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  2. Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-AF647
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  3. Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-UNLB
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  4. ELISA plate was coated with purified rabbit IgG Fab, IgG Fc, IgG, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit Light Chain-UNLB (SB Cat. No. 4060-01) followed by Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-HRP (SB Cat. No. 1090-05).
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  5. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
    Rabbit IgG-UNLB
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  6. ELISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-UNLB (SB Cat. No. 4020-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  7. Swine Anti-Rabbit IgG(H+L), Mouse/Rat/Human/Goat SP ads-UNLB
    Swine Anti-Rabbit IgG(H+L), Mouse/Rat/Human/Goat SP ads-UNLB
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  8. ELISA plate was coated with purified rabbit IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit Ig, Human ads-UNLB (SB Cat. No. 4010-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/ Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat Anti-Rabbit Ig, Human ads-UNLB
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  9. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  10. ELISA plate was coated with purified rabbit IgG Fc, IgG Fab, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fc-UNLB (SB Cat. No. 4041-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  12. ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 1038-05).
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  13. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-UNLB (SB Cat. No. 4030-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Rabbit IgG-UNLB
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  14. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with Swine Anti-Rabbit IgG(H+L)-UNLB (SB Cat. No. 6311-01) followed by Goat Anti-Porcine IgG(H+L)-HRP (SB Cat. No. 6050-05).
    Swine Anti-Rabbit IgG(H+L)-UNLB
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  15. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-AF488 (SB Cat. No. 6415-30) and DAPI.
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  17. ELISA plate was coated with purified rabbit IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG(H+L), Human ads-UNLB (SB Cat. No. 4051-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  18. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  19. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 1038-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT
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  20. ELISA plate was coated with purified rabbit IgG and IgM and mouse, rat, human, goat, hamster, bovine, horse, sheep, chicken, and guinea pig serum. Immunoglobulins and sera were detected with Swine Anti-Rabbit IgG(H+L), Multi-Species SP ads-UNLB (SB Cat. No. 6312-01; 1 mg/mL) followed by Goat Anti-Porcine IgG(H+L)-HRP (SB Cat. No. 6050-05).
    Swine Anti-Rabbit IgG(H+L), Multi-Species SP ads-UNLB
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  21. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG-UNLB (SB Cat. No. 4090-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
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  24. ELISA plate was coated with serially diluted Rabbit F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0112-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG F(ab')<sub>2</sub>-BIOT and Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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