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Mouse Anti-Rabbit IgG(H+L), Mouse/Human/Goat/Sheep SP ads-BIOT

Cat. No.:
4091-08
Mouse Anti-Rabbit IgG(H+L)-Biotin antibody with minimal reactivity to mouse, human, goat, and sheep serum proteins for use in ELISA assays.
$105.00
Size Price (USD) Quantity
1.0 mg $105.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Mouse IgG
Isotype Control Mouse IgG-BIOT
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Ascites from mice hyperimmunized with rabbit Ig
Cross Adsorption Mouse, human, goat, and sheep serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796007

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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  1. ELISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG-BIOT (SB Cat. No. 0107-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Mouse IgG-BIOT
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  2. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
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  3. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-BIOT (SB Cat. No. 4054-08) followd by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-BIOT
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  4. ELISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgM-BIOT
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    $132.00
  5. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
    Rabbit IgG-UNLB
    0111-01
    $102.00
  6. ELISA plate was coated with purified rabbit IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit Ig, Human ads-BIOT (SB Cat. No. 4010-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit Ig, Human ads-BIOT
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  7. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  8. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
    Streptavidin-AP
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  9. ELISA plate was coated with purified rabbit IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgG Fc-BIOT
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  10. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgG-BIOT
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  11. Rabbit peripheral blood lymphocytes were stained with Rat Anti-Rabbit Ig-BIOT (SB Cat. No. 4065-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
    Rat Anti-Rabbit Ig-BIOT (SB87a)
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  12. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
    Mouse Anti-Biotin-FITC (SB58c)
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    $165.00
  13. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Rabbit IgM-UNLB
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  14. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG-BIOT (SB Cat. No. 4090-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Rabbit IgG-BIOT (2A9)
    4090-08
    $182.00
  15. ELISA plate was coated with purified rabbit IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fab-BIOT (SB Cat. No. 4040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgG Fab-BIOT
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  16. ELISA plate was coated with serially diluted Rabbit F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0112-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG F(ab')<sub>2</sub>-BIOT and Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Rabbit F(ab')2 IgG-UNLB
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  17. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4052-08) followed Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT
    4052-08
    $94.00